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CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2020, Vol. 42 ›› Issue (6): 1058-.doi: 10.19802/j.issn.1007-9084.2020222

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Cloning and functional study of peanut plasma membrane H+-ATPase gene AhHA1

  

  1. 1. College of Life Sciences, Shandong Normal University, Jinan 250014,China; 2. Biotechnology Research Center,Shandong Academy of Agricultural Sciences/ Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan 250100,China
  • Online:2020-12-28 Published:2020-11-19

Abstract:  Plasma membrane H+-ATPase plays a crucial role in the transmembrane transport system. As the
only proton pump which mediates ATP-dependent H+ extrusion to the extracellular space, thus generates electro⁃
chemical proton gradients across the plant plasma membrane. It provides driving force for a large set of secondary
transporters. In this study, a salt-induced plasma membrane H+-ATPase gene AhHA1 was identified. Homologous
gene analysis, phylogenetic tree analysis, subcellular localization analysis and tissue specific analysis were carried
brane H+-ATPase family, and provided candidate gene for improving the salt-tolerance ability of peanut.
out. Blast analysis showed that the cultivar peanut genome encoded 24 AhHA1 homologs. The phylogenetic tree anal⁃
ysis showed that the 24 AhHA1 homologs were divided into 5 subfamilies, and the highest homology was between
peanut AhHA1 and Arabidopsis thaliana AHA4 and AHA11. Subcellular localization analysis showed that AhHA1 lo⁃
cated on the plasma membrane (PM). Tissue specific expression pattern analysis showed that AhHA1 was highly ex⁃
pressed in roots. To further understand the function of AhHA1 in salt resistance, AhHA1 gene was transformed into
Arabidopsis thaliana, and its expression increased the salt tolerance of transgenic Arabidopsis lines both in germina⁃
tion stage and post-germination stage. The transgenic Arabidopsis lines also showed greater biomass than wild-type
plants under salt stress condition. The results showed that AhHA1 could increase salt-tolerance ability of transgenic
plants. This study increased our knowledge about the peanut salt-tolerance mechanism and peanut plasma mem⁃

Key words: Arachis hypogaea, plasma membrane (PM) H+-ATPase, AhHA1, salt tolerance

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