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  • 2016 Volume 38 Issue 4
    Published: 28 August 2016
      

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  • CAI Dong-fang, ZHANG Shu-fen, XIAO Ying-jie, WU Jiang-sheng, LIU Ke-de*
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    Association mapping is a powerful approach to detect the genetic basis of complex quantitative traits with high resolution. To unravel the genetic basis of oleic acid, linoleic acid and linolenic acid in oilseed rape, and search the potential candidate locus underlying these fatty acid component traits, a panel of 192 inbred lines of Brassica napus was genotyped using 1 109 single-locus microsatellite markers and amplified fragment length polymorphism (AFLP) markers. Using the optimal Q+K model, association analyses were carried out for oleic acid, linoleic acid and linolenic acid in 3 years. A total of 93 associated loci were detected. The effects of phenotypic variance explained by associated markers were between 3.04% - 38.34%. Among them, 49 markers were repeatedly detected at least in 2 years. 15 common markers were repeatedly detected with correlated traits in the association mapping. The combination of favorable alleles of multiple associated markers could enhance trait performance of rapeseed variety for fatty acid components traits.

  • YANG Yong-xue,LI Zhen-bo, TONG Chao-bo, HUAN Jun-yan, YU Jing-yin, DONG Cai-hua*, LIU Sheng-yi
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    To better understand the function of Brassica napus gene BnCP51, specific primers were designed according to genomic information, BnCP51 gene and its promoter were cloned from Brassica napus L. cv Zhongshuang 9. The complete open reading frame of BnCP51 (KC898325 in GenBank) is 1 032bp in length. The promoter of BnCP51 is 1 951bp in length. BnCP51 was shown to be specifically expressed in Zhongshuang 9 buds by qRT-PCR. To investigate the expression pattern of BnCP51 promoter, a recombinant vector was designated as pBnCP51::GUS and transformed into Arabidopsis. GUS assay results showed that the promoter was specifically observed in buds and anthers at middle stage. No GUS expression was observed in roots, stem, leaves or siliques. It suggested that BnCP51 played important role in bud and anther development in B. napus.

  • LI Xin-ping, XU Zhi-jun, CAI Yan, GUO Jian-bin, HUANG Li, REN Xiao-ping, LI Zhen-dong, CHEN Wei-gang, LUO Huai-yong, ZHOU Xiao-jing, CHEN Yu-ning,WU Ming-yu, JIANG Hui-fang,*
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     Peanut is one of the important commercial and oil crops in the world, and it is one of the key sources of edible vegetable oils. High oil content and reasonable fatty acid composition are the main objects of genetic improvement of peanut quality. In this study, 188 recombinant inbred lines (RIL), derived from a cross between two Spanish type peanut cultivars (Yuanza 9102 × Xuzhou 68-4), and their parents were planted in Wuhan with two replications in three consecutive years. The content of oil, oleic acid, and linoleic acid were measured. The RIL population showed abundant phenotypic variation. Compared with their parents, one line had higher oil content and 23 lines had higher oleic acid content. Genetic analysis showed that the broad sense heritability of oil content, oleic acid, linoleic acid and O/L were 0.849, 0.761, 0.874 and 0.887, respectively, which indicated that genetic factors were predominant in determining the phenotypes of these traits. 82 QTLs, distributed on 11 linkage groups, were identified for the four traits, including 15 QTLs for oil content, 21 QTLs for Oleic acid, 21 QTLs for Linoleic acid and 25 QTLs for O/L. Each QTL explained 3.71%-28.14% of the phenotypic variation. There were 23 major QTLs (R2>10%), 8 QTLs identified in two years repeatedly, and 4 QTLs were identified in three years repeatedly. Among them, 7 major QTLs and 5 repeatable QTLs were first reported, especially, the marker interval GM2839-GNB159 on LG02, were identified for the three traits, including Oleic acid and O/L in three years repeatedly, Linoleic acid in two years repeatedly, each QTL explained 5.80~28.14% of the phenotypic variation, which would have great significance for marker-assisted breeding of high quality peanut.

  • HUANG Tao-cui, LI Jia-na*, TANG Shi-yi
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    To understand the stability of agronomic traits and their relations to yield stability and adaptability, genotype × environment interaction was analyzed by using candidate canola varieties tested in regions along Midway of Yangtze River in China during 2014-2015. Varieties in 4 tested groups (12 in each group) were planted in randomized complete blocks in 3 replications at 10 sites. Genotype × environment interaction (G×E) was analyzed by using AMMI (additive main effects and multiplicative interaction) and regression models. AMMI analysis of variance showed highly significant genotype and environment mean squares, suggesting that canola yield of genotypes were under the major environmental effects of G×E. Results revealed that the yield and agronomic traits of high-yield type and medium-yield type were more stable. Low-yield type had moderate stable or unstable agronomic traits, and was not stable in yield. Stability of agronomic traits and yield were significantly correlated. The joint linear stability effect of agronomic traits could explain ~70% of yield stability. Significant correlations were found between stabilities of primary branch height and relative height of primary branch (the ratio of branch height to plant height), and also between stabilities of primary branch number and silique number per plant. Close relationship was found between the stabilities of yield and of 1 000-seed weight, primary branch number, silique density on the main inflorescence, relative branch height, seed per silique, growth duration and Sclerotinia disease index.

  • Gong Shen Zhang Pan-pan, Feng Nai-jie, Zheng Dian-feng*, Li Dong, Zhang Hong-peng, Liu Wen-bing, Liu Yang
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    In order to find the key point in time and spatial location in soybean root growth, root spatial and temporal distribution characteristics of soybean cultivars with different growth habits were studied. Hefeng 50 (semideterminate) and Hefeng 55 (indeterminate) were planted in two kinds of cultivation device, their root length, root surface area, root volume and root dry weight in different layers of vertical and horizontal direction were analyzed at V6, R2, R4, R5, R6, R7 stages. Results showed that the total root length, total root surface area and total root volume showed unimodal growth trend peak with the advance of growth stages, the peak appeared at R4 to R6 stage. The root dry weight density, length density, surface area density, and volume density decreased gradually with soil depth increase, while these indicators first decreased and then increased with the increase of distance from the main stem, and they reached a maximum 0~20cm in vertical and 0~5cm in horizontal respectively. The root length density, surface area density, and volume density in the vertical 0~20cm soil depth at different growth stages accounted for 49.05%~66.09%, 52.07%~66.66%, 56.74%~69.73% and 79.14%~84.82% of their respective total, and in the horizontal 0~5cm of distance from the main stem accounted for 28.79%~41.68%, 41.12%~59.24%, 58.44%~76.64% and 88.85%~94.02% of their respective total. Hefeng 50 had more developed root system, root volume, greater dry matter accumulation than Hefeng 55. Therefore, root indexes from R4 to R6 stage could be used as reference in the high-yield soybean breeding.

  • WU Yu-shan, GONG Wan-zhuo, LIU Xin, WU Xiao-ling, Yang Feng, WANG Xiao-chun, LIU Wei-guo, YONG Tai-wen, YANG Wen-yu
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    To explore the methods on evaluating and analyzing compensation growth ability, and to compare difference among soybean cultivars in light recovery period in relay intercropping, 136 soybean cultivars (lines) were planted in a field experiment under natural light condition and shade+light recovery condition. 14 morphology traits were investigated on 30 days after light recovery, the yield of soybean per plant was investigated at the mature period. Principal component analysis, membership function method, cluster analysis and stepwise regression analysis were used to comprehensively evaluate the compensation growth ability based on compensation coefficient of all indexes calculated from both treatments. Results showed that 4 independent comprehensive components were extracted from 14 single indexes by principal component analysis. 136 soybean cultivars (lines) were divided into 5 groups, the compensation growth ability of the Ⅵ group (10 cultivars) showed the strongest compensation growth ability and the highest yield. The mathematical evaluation model for soybean compensation growth ability was established as D=-1.314+1.110 X4+0.623 X7+0.831 X1+0.642 X9, (R2=0.990). Based on the model, aboveground dry weight, leaf area, SPAD, and middle section diameter could be used for identification index of soybean varieties to compensation growth ability.

  • YU Xiao-bo, LIANG Jian-qiu, ZHANG Ming-rong, WU Hai-ying, FENG Jun, MING Chong, TANG Qiong-ying, LI Xiao-qing
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     Leaf morphological characteristics and photosynthetic traits were studied to investigate the leaf response to changed environment in relay strip intercropping system. Three varieties with different shade-tolerance, ND12, GX1 and HX5 were used in the field experiment to investigate leaf angle, petiole length, leaf index, photosynthetic characteristics in vivo and specific leaf weight, leaf area per plant. Compared with monoculture, the results showed that leaf angle increased significantly in relay strip intercropping system, and the maximum increase was in upper layer at seedling and flowering stage, in lower layer at podding stage. Leaf index also increased significantly at seedling and flowering stage, and the response degree of soybean leaf layers were different at different stages. As with the leaf index, leaf petiole length of seedling and flowering stage increased significantly, and petiole length rendered as upper>middle>lower. Photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), specific leaf weight (SLW) and leaf area per plant (LAP) all decreased significantly, but Ci increased. Among these 3 varieties, ND12 had the least increase in leaf angle, but the most increase in petiole length and the most decrease in SLW, LAP and photosynthetic rate. HX5 had the most increase in petiole length and the least increase in leaf angle, and the decrease of photosynthetic rate and LAP is the most. ND12, shade-tolerant variety, adjusted leaf morphology and position to increase light capture and light utilization. Accordingly, ND12 adapted well to relay strip intercropping system.

  • WANG Lei, TAN Mei-lian, Ye chun-lei, Luo Jun-jie, YAN Xin-chu*
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     Different planting patterns were designed to investigate linseed (oil flax, Linum usitatissimum L.) yield and water use efficiency in arid and semi-arid rainfed area in 2014 (normal rainfall) and 2015 (drought). These patterns included whole field plastic mulching with soil covering the top of mulch and bunch-seeding (T1), bunch-seeding directly on the double ridges and furrows mulched with plastic film system used in maize last season (T2), ridges mulched with plastic and row-seeding in furrow (T3), and two control, uncovered bunch-seeding (CK1) and uncovered row-seeding (CK2). In this study, earlier seedling emergency and shorter growth of duration(0-7d)were observed under mulching cultivation than uncovered plantings in two years. More shortened growth stages (16-20d) but with weaker effects on ripeness were displayed under all the planting models in drought year (2015) than in normal year (2014), and lower yield was obtained in 2015. It was suggested that the yields of linseed were significantly higher and WUE was also remarkably increased (P<0.05) in T1 and T2 patterns than in other 3 patterns. Moreover, income analysis showed that the best and the better profit were gained with T1 and T2 patterns respectively in both years. It was concluded that among the 5 patterns, T1 and T2 were optimal in arid and semi-arid regions with 350 mm rainfall.

  • LIU Kai-lou, HU Hui-wen, HU Zhi-hua, LI Da-ming, ZHOU Li-jun, HUANG Qing-hai*, YU Xi-chu, YE Hui-cai, XU Xiao-lin, WANG Sai-lian
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     To better forecast sesame yield, field experiment was carried out using different dicyandiamide (DCD) rates. DCD amounts were marked as different percentages of DCD to urea. They were D0 (0), D3 (3%), D5 (5%), D7 (7%), D10 (10%), D15 (15%) and D20 (20%). Trends of normalized difference vegetation index (NDVI) at 4 key growth stages [seedling, and 3 flowering stages (initial, full and late)] were investigated. Relationship between yield and NDVI at each stages was established and validated. Result showed that with DCD increasing, NDVI values were improved. But when DCD was more than 3%-10%, NDVI began to reduce. NDVI values at D3, D5 and D7 were higher in seedling and full flowering stages, and they were 6.2%-24.8% more than D0. During late flowering, the NDVI values of D5, D7 and D10 were the highest, and they were increased than D0 by 47.2%-103.1%. In the condition of equivalent N fertilizers, the fitting equations between NDVI value and yield were not similar at different stages. However, it was found that the fitting equations at full and late flowering stages [y = ln (0.258 7 + 6.968 7x) and y = ln (0.894 1 + 3.998 8x)] were significantly different (p < 0.01), which indicated that both equations could be used to predict sesame yield. In validation experiment, the error between the predicted and measure values in late flowering was small (R2 = 0.611 5, RMSE < 10% and RRMSE < 25%). Therefore, equation of late flowering was feasible to estimate sesame yield of different amount of DCD based on NDVI.

  • LIU Jia, ZAHNG Ji, QIN Wen-jing, YANG Cheng -chun, XIE Jie, WANG Fang-dong, XIA Wen-jian, XIANG Xing-jia, LIU Guang-rong, XU Chang- xu*
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     In order to study the effects of nitrogen (N) application and rhizobia inoculation on peanut yield, N uptake and utilization and economic benefit in red soil upland, peanut cultivar Yueyou 256 was used as the test material, a field randomized block experiment was carried out with 9 treatments, which were no N fertilizer (N0), 50% N (N50%), 75% N (N75%), 100% N (N100%, local traditional N application amount was 135 kg·hm-2), 125% N (N125%), 150% N (N150%), 50% N with rhizobia inoculation (N50%+RI), 75% N with rhizobia inoculation (N75%+RI), and 100% N with rhizobia inoculation (N100%+RI). Results showed that appropriate N application promoted growth of peanut, while excessive N would be adverse. Peanut yield increased 9.8%~13.5% under single N application, and reached maximum of 4 651.0 kg·hm-2 under N75% treatment. N application with rhizobia inoculation increased the yield by 0.7%-11.7% compared with corresponding single N application treatments. When N supply was in shortage (N50%), the effect of rhizobia inoculation was not obvious, and the yield slightly increased by 0.7%. The yield increased 11.4% and 11.7% respectively, under N application of (N75% and N100%) with rhizobia inoculation. Under the condition of this experiment, the effect of N application 101.25 kg·hm-2 with rhizobia inoculation was the best, its yield reached 5169.1 kg·hm-2, and it increased by 26.1% compared with N0 treatment. In addition, N content and uptake of peanut organ, N absorption efficiency of peanut, and the economic benefit were also improved significantly. In conclusion, appropriate N application with rhizobia inoculation was effective in increasing peanut yield and economic benefit. This research could provide theoretical support for high yield and high efficiency cultivation, scientific and rational fertilization of peanut in red soil upland in South China.

  • ZHENG Yong-mei,FENG Hao,WU Zheng-feng,JIANG Rong-feng,LI An-dong,WANG Xu-fang,WANG Cai-bin,SHI Cheng-ren1,SUN Xiu-shan*
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    To determinate the role of nitrogen fertilizer regulation on soil nitrogen supplying characteristics and nitrogen utilization efficiency of peanut, three nitrogen fertilizer regulations including based fertilizer (T2),based and topdressing fertilizer (T3),combining available and slow-release fertilizer (T4) were conducted with no nitrogen application as control (T1) in field. Results confirmed that, soil nitrate nitrogen content in 0-60cm soil layer significantly increased with application of nitrogen fertilizer. In N base fertilizer treatment, the content of nitrate in 0-60cm soil layer was higher at early growth stage and lower at maturity stage of peanut than those of other treatments respectively. While soil nitrate content was low at early growth stage, but maintained a high level at maturity in T2 and T3 treatments. Especially in T3 treatment, the proportion of nitrogen to leaf decreased significantly and proportion of nitrogen to pod increased at maturity stage,accordingly, the pod yield,nitrogen agronomy efficiency and partial factor productivity for applied N(PPF)were higher than those of T1 treatment. Therefore,application of combining available and slow-release nitrogen helped to maintain enough soil nitrogen supply at the late growth stage of peanut,and to improve nitrogen efficiency. 

  • LU Yan, LI Cheng, CHEN Zhi-de, YANG Yi-Hong, TANG Can-ming*
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    To determine effect of Bacillus amyloliquefaciens 41B-1 in suppression of stem rot disease of groundnut plants caused by Sclerotium rolfsii, the inhibitory efficiency of 41B-1 on hyphal growth of Sclerotium rolfsii on PDA were detected by coculture method. Under greenhouse conditions, we observed the resistant ability of groundnut plants treated with 41B-1 after a challenging inoculation with Sclerotium rolfsii. Results showed that strain 41B-1 significantly inhibited hyphal growth, destroyed hyphal structure and reduced the number of sclerotia. The results also showed that 41B-1 strain had good colonization ability in the roots of plants. In addition, the colonization of Sclerotium rolfsii in the roots of plants was reduced and the disease index was significantly lower than that in the control. 41B-1 strain exhibited greater than 90% biocontrol efficacy against Sclerotium rolfsii in groundnut plants under greenhouse conditions. 41B-1 strain provided a promising strategy to control stem rot disease of groundnut caused by the fungal pathogen Sclerotium rolfsii. 

  • LIN Xiao-min, TAN Xiao-rong, WANG Lian-zheng, LI Bin*, SUN Jun-ming*
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    To explore the role of isoflavones in resistance of soybean to soybean cyst nematode (Heterodera glycines, SCN), 2 resistant cultivars ( Huipizhiheidou and Wuzaiheidou ) and 2 susceptive cultivars (Zhonghuang 13 and Zhonghuang 35) were inoculated with cyst nematode race 4 ( SCN4). Their isoflavones in roots at 38d after inoculation were measured by HPLC. Results showed that isoflavone contents increased after SCN infection in all soybean varieties, but the induction patterns varied, especially in two resistant soybean varieties. It indicated that the mechanism of resistance to soybean cyst nematode might have multiple models, and isoflavone could be involved in some of these resistance mechanisms. 

  • FEI Wei-xin, WANG Shu-fen, LI Qiang-sheng, WU Xiao-yun, CHEN Feng-xiang, HOU Shu-min, RONG Song-bai, HAO Zhong-ping, GAO Zhi-mou*, HU Bao-cheng*
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    To better control the clubroot disease caused by Plasmodiophora brassicae, different sowing date of winter rapeseed was tested. Clubroot control and yield loss of 9 sowing dates were investigated under pots and field conditions during 2013 to 2015. Disease incidence and disease index of the plants at late sowing date from D6 (Oct. 19) to D9 (Nov. 3) were significantly lower than those of early dates from D1 (Sep. 24) to D5 (Oct. 14). Among these 9 sowing date treatments, rapeseed yield of D9 was the lowest, and D3 (Oct. 4) was the highest. Seed yield from D1 to D8 (Oct. 29) was not significant different on disease incidence. Thus the late sowing dates resulted low disease incidence. The results indicated that using late-tolerant cultivar at late-sowing date (about Oct. 20) could be beneficial in controlling clubroot of winter rapeseed, reducing total quantity of field resting spores and increasing the yield.

  • GAO Yu, HOU Xiang-jie, XU Wei, XIONG Jin-feng, CUI Juan, SHI Shu-sen*
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    19-spot ladybug, Harmonia axyridis ab. Ovemdecimpunctata (Coleoptera: Coccinellidae), is an important predatory natural enemy in soybean field in Changchun area. The predation responses of 19-spot ladybug to thrips Thrips nigropilosus (Thysanoptera: Thripidae) was investigated under laboratory condition. The results showed that the function responses of 19-spot ladybug to T. nigropilosus accorded with Holling Ⅱ Disk Equation, of which the regression equation was Na=0.9346N/(1+0.0339N). The predatory function of 19-spot ladybug on T. nigropilosus was restricted by the density of their population. The average consumption of the thrips decreased with the increase of density of 19-spot ladybug with the regression equation of A=13.534P-0.7029. Furthermore, there was intraspecies interference among 19-spot ladybug and the interference regression equation was E=57.218P-1.246. The 19-spot ladybug showed a preferable predatory potential on controlling T. nigropilosus. These results could provide a guideline for field releasing of 19-spot ladybug.

  • LI Lin, CAO Ao-cheng*, YU Da-zhao*,ZHU Wen-da, YAN Dong-dong, OUYANG Can-bin
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    To better control the invasive species Crofton weed (Eupatorium adenophorum Spreng), field experiments were conducted in Xichang, Sichuan Province to determine the possibility of replacement control of E. adenophorum Spreng with rapeseed (Brassica napus L.). The control effect and economic benefits were evaluated in this study. Results showed that the plantation of rapeseed in the ploughed fallow provided good control of E. adenophorum, which significantly reduced the consumption of water and fertilizer by E. adenophorum. Rapeseed yield increased with the planting density, ranged from 2 830.75 to 5 332.28 kg/hm2. Economic benefits reached 14 153.75 to 26 661.38 yuan/hm2. Appropriate planting density could bring good economic benefits for farmers. Therefore, rapeseed planting played an important role in the replacement control of E. adenophorum and increased farmer incomes. 

  • CUI Chao,WANG Jing*,WANG Hai-wei,ZHENG Xi-qing,DI Na,SU Zhi-fang,LI Ya-zhen
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     Broomrape occurrence on sunflower becomes a serious threat. To better understand the damage of broomrape parasitism in whole growth period of sunflower, root and shoot physiological characteristics, dry matter accumulation, yield and yield components in 3 different parasitism severity of sunflower broomrape were investigated in 2 sites. Cultivar SH363 was used as plant material. Results showed that broomrape parasitism significantly reduced sunflower yield. In light parasitism (L-H) treatment broomrape, yields were 3.66 t·hm-2 and 3.23 t·hm-2 respectively in 2 sites, declined 24.85% and 16.32% comparing with control yield. In heavily harm (H-H) parasitism treatment of broomrape, yields were 2.70 t·hm-2 and 1.98 t·hm-2 respectively in 2 sites, declined 44.56% and 48.70% comparing with control yield. Broomrape parasitism significantly decreased the number of harvest, full grain number per disk, full grain weight per disk, hundred-grain weight. Dry matter accumulation during anthesis and maturity was significantly decreased. Root biomass especially at 20-40 cm-soil layer quickly decreased during late growth period. Thus, root growth was limited, leaf area index and photosynthesis accumulation declined as the sunflower broomrape parasitizm damage increased, resulting in a lower grain yield.

  • SHANG Chang-hua,,WANG Zhong-ming,YUAN Zhen,WAN Xia *
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    To modifying oleaginous Dunaliella parva for biodiesel, transcriptome of Dunaliella parva was sequenced under nitrogen limitation and nitrogen sufficient condition by Illumina Hiseq 2000 platform. 1 529 differentially expressed genes were obtained. Analalysis resluts indicated that many differentially expressed genes were involved in endocytosis, glycerophospholipid metabolism, ether lipid metabolism, the biosynthesis, transport and process of genetic materials, biosynthesis of secondary metabolites and nitrogen metabolism. 

  • WANG Xue-fang, LI Pei-wu*, WANG Xiu-pin*, SUN Xiao-man,ZHANG Liang-xiao, MA Fei
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     For high precision of herbicide detection in oilseed rape, high performance liquid chromatography-triple quadrupole mass spectrometry was applied for determination of tribenuron-methyl (TBM) and metsulfuron-methyl (MSM) in rapeseeds. Ultrasonic extraction of TBM and MSM were carried out at 15℃ with an extraction time of 3 min. The separation was performed in isocratic mode on a C18 column with a mobile phase containing acetonitrile-aqueous solution (0.1% formic acid). Sensitive detection of TBM and MSM by electrospray ionization mass spectrometry (ESI-MS) was carried out on the basis of selected reaction monitoring (SRM) mode. Effect of standard addition method on the matrix effects in LC-MS of TBM and MSM was discussed. Recoveries of TBM and MSM were in the range of 89.0%-106.5%. The intra-day and inter-day precision were less than 4.3% and 5.3% respectively. 

  • WU Xin-jie, CHEN Feng-xiang, HU Bao-cheng, LI Qiang-sheng, JIANG Ying-fen, RONG Song-bai, HUANG Xiao-rong
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    To better understand the stability of recessive epistatic genic male sterile lines in Brassica napus L., effects of heat and sowing dates were investigated on sterile stability of 9012A (homozygous genotype) and 12-204A (heterozygous genotype) of B. napus. Results showed that fertility reversion was observed under certain high temperature stress, and the heat sensitivity was obviously different between the 2 lines. For 9012A, the critical temperature of fertility reversion was between 30 and 35℃. Fertility reversion occurred under continuous treatments of 16-32 h, or intermittent treatments of 3h/d×6d, 6h/d×5d, 9h/d×4d, 12h/d×3d and 15h/d×2-4d at 40℃, or intermittent treatments of 15h/d×2-4d at 35℃. Among the intermittent treatments, condition of 15 h per day at 35℃ with consecutive 3d caused the most obvious reversion. Under natural weather condition, 9012A was completely stable at 6 sowing dates (from Oct 16, 2014 to Feb 9, 2015). For 12-204A, the critical temperature of fertility reversion was 29℃. Continuous treatment of 15h at 35℃ led to the most obvious fertility reversion. Under natural weather condition, 12-204A fertility obvious reversed at all 6 sowing dates above. Weak selfing seedset of both 9012A and 12-204A were achieved under heat treatment, but with low yield.

  • CHEN Si-wei,ZHANG Ting-ting,XIAO Li,WU Xiao-ling*,YANG Wen-yu*
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    Phytophthora root rot of soybean seriously damaged the soybean production in Renshou and Yaan of Sichuan Province. Four isolates of Phytophthora sojae(RS1, RS2, YA1, YA2)were isolated by using baiting method from soil and tissue isolated from diseased plants. Their virulence determination was carried out on 11 differentials host containing known resistant genes. Results showed that YA1 and YA2 had the same virulence formula of 1a,1b,1d,2,3c, indicating their same pathotype. The virulence formula of RS1 and RS2 were 1a,1d,1k,3a,3c,4,5 and 1a,1b,1c,1d,1k,2,3c,5, respectively. 157 soybean germplasm were inoculated with 3 pathotypes of P.sojae. Results showed that local varieties had more resistance than cultivated variety, however, the amount of resistant germplasms accounted for only 23.57%. The research could provide useful information for resistant breeding.