QTL mapping and candidate genes analysis for flowering time in rapeseed (Brassica napus L.)

WEI Ya-qi,WEI Wen-liang,LIU Dao-min,ZHANG Jiang-jiang,ZHAN Jie-peng,SHI Jia-qin*, WANG Xin-fa,LIU Gui-hua

CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2019, Vol. 41 ›› Issue (5) : 679.

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CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2019, Vol. 41 ›› Issue (5) : 679. DOI: 10.19802/j.issn.1007-9084.2019051

QTL mapping and candidate genes analysis for flowering time in rapeseed (Brassica napus L.)

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Abstract

For molecular marker-assisted breeding on early-mature rapeseed, BnaZNF2 population containing 184 individuals was constructed using the sequenced rapeseed cultivar ZS11 and re-sequencing rapeseed line No. 73290 as parents. Flowering time QTLs were detected and integrated using both genotype and phenotype data of F2:3 family in Wuhan for 3 years (2010-2012). 14 QTLs were mapped for flowering time distributed on 11 linkage groups. 5 QTLs were detected repeatedly in 3 years, including qDtF.A2-1, qDtF.A6-2, qDtF.C2-1, qDtF.C2-2 and qDtF.C3-1. Their contributions were between 7.1% and 21.1%.Through literature reviewing and searching on web? sites on Arabidopsis and rice, 442 genes related to plant flowering time were collected. Based on the physical map of rapeseed genome, 54 putative candidate genes were envolved in the QTL intervals by bioinformatics analysis. 2 to
8 candidate genes were mapped respectively on 5 major QTL intervals, 15 of which had sequence differences be? tween parents. The 15 putative candidate genes for flowering time could be developed into functional markers for molecular marker-assisted selection and plant breeding.

Key words

Brassica napus L / early-mature rapeseed / flowering time / QTL / candidate genes

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WEI Ya-qi,WEI Wen-liang,LIU Dao-min,ZHANG Jiang-jiang,ZHAN Jie-peng,SHI Jia-qin*, WANG Xin-fa,LIU Gui-hua . QTL mapping and candidate genes analysis for flowering time in rapeseed (Brassica napus L.)[J]. CHINESE JOURNAL OF OIL CROP SCIENCES, 2019, 41(5): 679 https://doi.org/10.19802/j.issn.1007-9084.2019051

References

[1] Hu Q,Hua W,Yin Y,et al. Rapeseed research and pro?duction in China[J]. Crop J,2017,5(2):127-135.
[2] 王汉中,殷艳. 我国油料产业形势分析与发展对策建议[J]. 中国油料作物学报,2014,36(3):414-421.
[3] 沈金雄,傅廷栋. 我国油菜生产、改良与食用油供给安全[J]. 中国农业科技导报,2011,13(1):1-8.
[4] Li L,Long Y,Zhang L B,et al. Genome wide analysisof flowering time trait in multiple environments via highthroughput genotyping technique in Brassica napus L[J]. PLoS ONE,2015,10(3):e0119425.
[5] Campbell D C,Kondra Z P. A genetic study of growth characters and yield characters of oilseed rape[J]. Eu? phytica,1978,27(1):177-183.
[6] 高永同.甘蓝型早熟油菜的花期选择[J].湖北农业科学,1979,18(11):20-25.
[7] 蔡长春,傅廷栋,陈宝元,等. 甘蓝型油菜遗传图谱的构建及开花期的QTL分析[J]. 中国油料作物学报, 2007,29(1):1-8.
[8] Long Y,Shi J,Qiu D,et al. Flowering time quantitative trait loci analysis of oilseed Brassica in multiple environ?ments and genomewide alignment with Arabidopsis[J]. Genetics,2007,177(4):2433-2444.
[9] Raman H,Raman R,Eckermann P,et al. Genetic and physical mapping of flowering time loci in canola(Brassi? ca napus L.)[J]. Theor Appl Genet,2013,126(1): 119-132.
[10] Wei D Y,Mei J Q,Fu Y,et al. Quantitative trait loci analyses for resistance to Sclerotinia sclerotiorum and flowering time in Brassica napus[J]. Mol Breeding, 2014,34(4):1797-1804.
[11] Nelson M N,Rajasekaran R,Smith A,et al. Quantita? tive trait loci for thermal time to flowering and photoperi? od responsiveness discovered in summer annual-type Brassica napus L[J]. PLoS ONE, 2014, 9(7): e102611. DOI:10.1371/journal.pone.0102611.
[12] 李保军,王灏,王晓东,等. 甘蓝型油菜开花性状QTL分析[C]//中国作物学会——2015年学术年会论文集. 黑龙江哈尔滨,2015:69.
[13] Liu H D,Du D Z,Guo S M,et al. QTL analysis and the development of closely linked markers for days to flower? ing in spring oilseed rape(Brassica napus L.)[J]. Mol Breeding,2016,36(5):52.
[14] Li B J,Zhao W G,Li D R,et al. Genetic dissection of the mechanism of flowering time based on an environ? mentally stable and specific QTL in Brassica napus[J]. Plant Sci,2018,277:296-310.
[15] Rahman H,Bennett R A,Kebede B. Molecular map? ping of QTL alleles of Brassica oleracea affecting days to flowering and photosensitivity in spring Brassica napus [J]. Plos One,2018,13(1):e0189723. DOI:10.1371/ journal.pone.0189723.
[16] Shen Y S,Xiang Y,Xu E S,et al. Major Co-localized QTL for plant height,branch initiation height,stem di? ameter,and flowering time in an alien introgression de? rived Brassica napus DH population[J]. Front Plant Sci, 2018,9:390.
[17] Mei D S,Wang H Z,Hu Q,et al. QTL analysis on plant height and flowering time in Brassica napus[J]. Plant Breeding,2009,128(5):458-465.
[18] Zhang F Q,Huang J Y,Tang M Q,et al. Syntenic quan? titative trait loci and genomic divergence for Sclerotinia resistance and flowering time in Brassica napus[J]. J In? tegr Plant Biol,2019,61(1):75-88.
[19] Jian H J,Zhang A X,Ma J Q,et al. Joint QTL mapping and transcriptome sequencing analysis reveal candidate flowering time genes in Brassica napus L[J]. Bmc Ge? nom,2019,20:21.
[20] Wang N,Chen B Y,Xu K,et al. Association mapping of flowering time QTLs and insight into their contribu? tions to rapeseed growth habits[J]. Front Plant Sci, 2016,7:338.
[21] Xu L P,Hu K N,Zhang Z Q,et al. Genome-wide asso? ciation study reveals the genetic architecture of flowering time in rapeseed(Brassica napus L.)[J]. DNA Res, 2015:dsv035.
[22] 魏大勇. 甘蓝型油菜遗传结构分析和选择驯化研究 [D]. 重庆:西南大学,2015.
[23] Raman H,Raman R,Coombes N,et al. Genome-wide association analyses reveal complex genetic architecture underlying natural variation for flowering time in canola [J]. Plant Cell Environ,2016,39(6):1228-1239.
[24] Zhou Q H,Han D P,Mason A S,et al. Earliness traits in rapeseed(Brassica napus):SNP loci and candidate genes identified by genome-wide association analysis [J]. Dna Res,2018,25(3):229-244.
[25] Putterill J,Laurie R,MacKnight R. It's time to flower: the genetic control of flowering time[J]. BioEssays, 2004,26(4):363-373.
[26] Fornara F,de Montaigu A,Coupland G. SnapShot:con? trol of flowering in Arabidopsis[J]. Cell,2010,141(3): 550-550.e2.
[27] Srikanth A,Schmid M. Regulation of flowering time:all roads lead to Rome[J]. Cell Mol Life Sci,2011,68 (12):2013-2037.
[28] Robert L S,Robson F,Sharpe A,et al. Conserved struc? ture and function of the Arabidopsis flowering time gene CONSTANS in Brassica napus[J]. Plant Mol Biol, .
[29] 邹潇潇.甘蓝型油菜开花关键调控因子BnFLC基因家族的克隆和分析[D].武汉:华中农业大学.2012.
[30] Wang J,Long Y,Wu B D,et al. The evolution of Brassi? ca napus FLOWERING LOCUST paralogues in the con? text of inverted chromosomal duplication blocks[J]. BMC Evol Biol,2009,9(1):271.
[31] Wang N,Qian W,Suppanz I,et al. Flowering time vari? ation in oilseed rape(Brassica napus L.)is associated with allelic variation in the FRIGIDA homologue BnaA. FRI.a[J]. J Exp Bot,2011,62(15):5641-5658.
[32] 周晓晨,韩洪强,陈火英,等. 甘蓝型油菜生物节律钟输出基因BnGI的克隆和表达分析[J]. 上海交通大学学报:农业科学版,2014,32(5):5-11.
[33] 侯锦娜. 甘蓝型油菜开花QTL-qFT10-4-的图位克隆
及候选基因BnFLC.A10 的表达调控研究[D]. 武汉: 华中农业大学,2013.
[34] 陈磊. 甘蓝型油菜开花基因BnFLC.A2的图位克隆和功能分析[D]. 武汉:华中农业大学,2017.
[35] Sun F M,Fan G Y,Hu Q,et al. The high-quality ge? nome of Brassica napus cultivar‘ZS11’reveals the intro? gression history in semi-winter morphotype[J]. Plant J, 2017,92(3):452-468.
[36] Huang S M,Deng L B,Guan M,et al. Identification of genome-wide single nucleotide polymorphisms in allo? polyploid crop Brassica napus[J]. BMC Genom,2013, 14(1):717.
[37] Shi J Q,Zhan J P,Yang Y H,et al. Linkage and region? al association analysis reveal two new tightly-linked ma? jor-QTLs for pod number and seed number per pod in rapeseed(Brassica napus L.)[J]. Sci Rep,2015,5: 14481.
[38] Zeng Z B. Theoretical basis for separation of multiple linked gene effects in mapping quantitative trait loci[J]. Proc Natl Acad Sci,1993,90(23):10972-10976.
[39] Zeng Z B . Precision mapping of quantitative trait loci [J]. Genetics,1994,136(4):1457-1468.
[40] 易斌,陈伟,马朝芝,等. 甘蓝型油菜产量及相关性状的QTL分析[J]. 作物学报,2006,32(5):676-682.
[41] McCouch S R ,Cho Y G ,Yano M ,et al. Report on QTL nomenclature[J]. Rice Genet Newsl,1997,14 (11):11-13.
[42] Goffinet B,Gerber S. Quantitative trait loci:a metaanalysis [J]. Genetics,2000,155(1):463-473.
[43] 方宣钧,吴为人,唐纪良. 作物D方宣钧,吴为人,唐纪良. 作物DNA 标记辅助育种 [M]. 北京:科学出版社,2001

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