为了提高菜籽浓缩蛋白的生产效率以及蛋白含量，以预浸压榨菜籽粕为原料，研究单仓高效平筛制备菜籽浓缩蛋白的工艺。通过对原料的粒径分布以及不同粒径原料的成分分析得到原料粒径分布的R-R分布函数；对原料蛋白质与植酸的含量进行了相关性分析，结果显示两者具有显著的相关性（r=0.994 1）。利用单仓高效平筛制备菜籽浓缩蛋白，以筛下物得率和筛下物蛋白含量为指标研究进料流量和平筛旋转速度对筛分效果的影响，确定制备菜籽浓缩蛋白的最佳工艺为：进机流量为2kg/min，平筛回转速度为260r/min，在最优工艺条件下得到的菜籽浓缩蛋白的蛋白含量为56.99%(干基)，得率为26.66%，经醇洗脱毒后得到的菜籽浓缩蛋白的蛋白含量可达60.155%。

 In maize-soybean intercropping system, soybean was shaded by the taller crop maize, and consequently, soybean seeds were under shade stress conditions during whole developmental stages. Subsequently, whether and how the shade stress influence the abiotic stresses tolerance of soybean seeds during germination? It is worthy of further study. In this study, soybean cultivar “Hedou19” and maize cultivar “Jundan26” were employed as the experimental materials. The maize-soybean intercropping (IC) system was two rows of maize and soybean crossed planting (1:1), and the soybean monocropping (MC) was as control. After soybean was harvested, the quality traits including 100-seed weight, protein content and fat content were measured and the ability of resisting adversity (high temperature, mannitol, glucose, PEG, NaCl and abscisic acid) during germination was analyzed. Finally, the expression of genes related to signal transduction of ABA was further analyzed by qRT-PCR assay. Results showed that there was no significant difference in 100-seed weight, fat content and protein content between monocropping and intercropping soybean seeds. Further, intercropping soybean seeds germinated faster than monocropping soybean seeds under abiotic stresses conditions (high temperature, mannitol, glucose, PEG, NaCl and ABA) and showed strong resistance to abiotic stresses. Further qRT-PCR demonstrated that the transcription levels of GmABI4 and GmABI5, positive regulators in abscisic acid (ABA) signaling pathway, in the intercropping soybean seeds were lower than that in monocropping soybean seeds. Therefore soybean quality did not change in maize-soybean intercropping system, it could enhances the abiotic stresses tolerance during germination processes, maybe primarily through weaken ABA signaling strengthen.

以引进、自育的68份大豆品种资源为材料，在新疆石河子地区对18个农艺性状进行变异系数、相关分析、主成分分析及聚类分析。结果表明：在参试品种中，抗倒伏级数变异系数最大（40.53%），蛋白含量（3.43%）最小；产量与单株粒重、百粒重及脂肪含量呈极显著正相关（P<0.01），与生育期、结荚高度呈极显著负相关（P<0.01）；在主成分分析中，总共提取6个主成分，累计贡献率达到82.77%；聚类分析结果显示，在遗传距离为10处，可将参试的68份品种分为7个类群。类群Ⅰ，适应性较广，可在新疆大部分地区种植；类群Ⅲ，结荚量较大，单株粒数较多，蛋白含量较高；类群Ⅳ，具有一定的有效分枝数，单株粒数较多，蛋白含量较高；类群Ⅴ生育期较短，且产量较高，适合作为复播品种；类群Ⅵ，节数多，结荚量大，单株粒数、蛋白含量高，可作为本地区豆制品专用品种或芽用品种；类群Ⅶ，荚数较多，生物产量大，百粒重、脂肪含量较高，其生物产量较大，可作为麦后绿肥养地品种。试验结果可为新疆大豆种质资源引进、研究和利用提供参考。