Soybean [Glycine max (L.) Merill] is important source of plant protein and oil. Salt stress causes
yield loss which is a major abiotic stress to soybean. Identification of salt tolerant genes is important for breeding
salt tolerant soybean varieties. This paper summarized salt-tolerance related quantitative trait loci and genes ob⁃
tained through forward genetics, including GmCDF1 (Glyma. 08G102000, a major gene identified at germination
stage), 2 QTLs identified at emergence stage (located on chromosome 6 and chromosome 14 respectively), major
gene GmSALT3 (Glyma03g32900) and the QTL in G-linked group at seedling stage. Thus it was believed that no di⁃
rect correlation between salt tolerances to soybean at different growth stages. The paper also summarized research
progress of improving salt tolerance of soybean by reverse genetics, such as ion transport, transcriptional regulation
and the development of improving salt-tolerance by transferring exogenous genes. It is expected to provide referenc⁃
es for analyzing molecular mechanism of salt tolerance and for soybean breeding.

本文概述了紫苏在开发利用、种质资源收集评价、栽培与生理、活性物质提取、组织培养、转基因及基因克隆表达分析等生物技术研究方面的国内外研究进展情况，以期为紫苏的深入研究和进一步开发应用提供参考，有效促进紫苏产业的发展。

Glycerolipids are the main constituents of biological membranes, which participate in a variety of biological processes including energy and signal transduction and protein transport, thereby playing many important roles during plant growth and development. Glycerol-3-phosphate acyltransferase （GPAT） catalyzes the initial committed step in de novo biosynthesis of phosphatidic acid （PA）. PA not only serves as a general precursor for the biosynthesis of membrane glycerolipids and the neutral lipid triacylglycerols, but also acts as an important signal molecule. So far, however, it has remained unclear how many genes encoding GPATs are present in plants, mainly due to the lack of convenient and effective methods for identification of this enzyme. In this review, we analyze and summarize the recent advance in studies on glycerolipid biosynthesis as well as on cloning and characterization of GPAT genes, then introduce the methods used for characterizing GPATs, especially for the development and application of yeast genetic complementation, and finally present our prospects for future studies on the initial step of the glycerolipid biosynthetic pathway.

AGO(Argonaute) protein is a core component of RNA induced silencing complex (RISC) and plays
an important role in plant growth and development. The completion of peanut genome sequencing provides the con⁃
dition for analyzing the AGO gene family on the whole genome. The conserved domain of AGO protein was used to
obtain the members of the peanut AGO gene family which was based on the Peanut base database and NCBI. The
phylogenetic trees, physicochemical properties, chromosomal distribution, genes structure，domain structure, and
gene expression patterns in 22 tissues and under stresses were analyzed. The results showed that 51 members of the
peanut AGO genes including 12 of the A.duranensis genomes, 12 of the A.ipaensis genomes and 27 of the peanut cul⁃
tivars were identified,. Chromosomal location analysis showed that these members were unevenly distributed on the
peanut chromosomes, and 10 pairs of AGO members from the A.duranensis and A.ipaensis genomes had a significant
homology relationship. The expression pattern analysis shown that AdAGO2, AiAGO4, AdAGO3, AiAGO7, AdAGO8,
and AiAGO8 had high-level expression in 22 tissues of peanut; and the AGO gene family shown higher expression in

peanut shoot tip and stamens than other tissues. This study provided some theoretical evidence for further

revealing the function of AGO protein, and exploring targeted gene resources related to stress-resistant breeding in peanut.

Oil is the main energy storage substance and an important membrane component of plant. Oil is also involved in the signal transduction, stomatal opening and closing, pollination and fertilization, seed germination, and stress response. WRINKLED 1 (WRI1), a member of the AP2 transcription factor family, is master regulator in transcriptional control of plant oil biosynthesis. This paper reviewed recent advances in regulatory mechanism of WRI1 on plant oil biosynthesis, including (1) the discovery, origin, and evolutionary characteristics of WRI1; (2) gene expression characteristics, gene structure, protein structure, and promoter cis-acting elements of WRI1; (3) regulation mechanism of transcription level and translation level, and downstream target genes of WRI1; (4) the future research direction and application prospects. This review is expected to provide a reference for further understanding the molecular mechanism of WRI1 regulating plant oil synthesis, and also provide a theoretical basis for improving oil crops with WRI1.

种子大小是影响农作物产量的主要因素之一，提高种子产量对改善人类生活水平具有重要作用。研究控制种子大小的重要基因，并寻找高产相关的功能基因，对作物高产育种具有重要的理论意义和应用价值。目前，通过基因失活或超量表达分析，已经发现了一些控制种子大小的功能基因。这些功能基因参与胚、胚乳、珠被的发育过程，通过调控细胞的增殖和伸长程度控制种子的大小。本文主要综述现已鉴定的控制种子大小的一些重要基因。

Virgin coconut oil is a valuable source of medium chain fatty acids, with lauric acid comprising 44%-54% of the total fatty acids. With the deepening of research, virgin coconut oil has attracted growing attention in the field of food and medicine. Besides, its market scale is also expanding year by year. In this article, the nutrient components and physicochemical properties of VCO were introduced, and the health effects of VCO on antioxidant, antiviral, antibacterial and cardiovascular disease prevention and their potential mechanisms were reviewed. It was aimed to provide theoretical reference for the further development and popularization of virgin coconut oil.

本文在分析了我国食用植物油供给形势、油菜产业在食用植物油供给中的地位的基础上，对我国油菜产业的发展历史进行了回顾，提出在新的历史时期必须推动以“三高”（“高油、高产、高效”）为标志的我国油菜产业发展的第四次飞跃来应对新的挑战。

 Luteolin is a kind of natural flavonoids extracted from plants. It has multiple biological activities such as antioxidant, antiinflammation, anticancer, antipathogen and other physiological effects. Peanut shell is an ideal resource of luteolin with high content of flavonoid. As a renewable resource, peanut shell has wide application prospects in luteolin, if this compound could be extracted and purified efficiently. This paper reviewed physiological properties, bio-activities, biosynthesis and chemical synthesis of luteolin from peanut and other plants for further research of luteolin in peanut and other plants.

In order to explore the promoter function of AhGPAT9 gene involved in TAG de novo synthesis, a promoter sequence was cloned from the upstream of AhGPAT9 gene in peanut （Arachis hypogaea L.） cv Tifrunner genome, and bioinformatics analysis of it was carried out. After that, recombinant expression vectors of full-length promoter as well as several 5’-terminal deletion promoters fused with GUS reporter gene were constructed, respectively, and then transfected by Agrobacteriumrhizogenes mediated plant transgenic systems to analyze the promoter activity and expression pattern of AhGPAT9. It was found that the full-length of AhGPAT9 promoter sequence was 1750 bp, and its core region was located in -257 bp to -128 bp. In addition to the core elements essential for eukaryotic promoters, such as CAAT-box and TATA-box, the AhGPAT9 promoter also contained multiple cis-elements that were responsive to hormone regulation, stress induction, light response, endosperm specific expression and growth regulation. Furthermore, AhGPAT9 was mainly expressed on seedlings, stems, cauline leaves, flowers, 9-12DAP siliques and corresponding mature embryos of transgenic Arabidopsis by GUS histochemical staining test. Our findings provide a new theory for further revealing the biological functions of AhGPAT9 involved in the lipids biosynthetic pathway of peanut.

Soybean is one of the most important legume crop and the main source of plant protein and edible oil for human beings. Soybean mosaic virus(SMV) is one of the major pathogens widely distributing in the soybean-producing areas, which seriously affects yield and quality of soybean all over the world. At present, there is no effective chemical agent for controlling SMV disease. Breeding virus-resistant varieties is the most economical, safe, and effective approach, however, conventional breeding for disease resistance is time-consuming, and the resistance spectrum of the improved varieties is narrow and easily lost due to the viral mutations. Transgenic technology can broaden the genetic basis through utilizing the cross-species gene resources or creating novel genes via genome editing technology, and these advantages make it become a powerful method for antiviral researches in soybean, which could provide the crucial theoretical basis for soybean anti-disease breeding. Here, we have summarized the domestic and foreign research progresses of transgenic resistance to SMV disease including positive resistance, negative resistance, and pathogen-derived resistance, as a reference for related research.

植物油脂既具有重要生理功能，又有巨大食用和工业价值。油脂代谢是植物最重要的代谢途径之一，然
而这一代谢途径非常复杂，涉及到一系列生化反应及催化反应的酶。本文介绍了植物种子中油脂代谢的基本途
径，并从工业用和食用两方面对近年来植物油脂代谢的遗传调控与基因工程研究进展进行了较为详细的总结，同
时对植物油脂调控基因工程的发展作了展望。

从两个酸性土壤生态实验点（江西鹰潭、浙江金华）种植的不同大豆品种根瘤中，分离纯化出29个分离物，并对其进行了分子鉴定和生物学特性分析。从其中16个分离物中扩增出nifH基因，测序分析结果表明，分离纯化出的菌株主要为慢生根瘤(Bradyrhizobium)属的菌株。10个分离自浙江金华的菌株，在大豆品种之间的种属差异并不大。在6个分离自江西鹰潭的菌株中，耐铝和敏感的大豆品种根瘤菌株存在明显的基因型差异。通过基于nifH 基因的系统发育分析选取代表性菌株，并进行16S rDNA序列测定和菌株回接结瘤实验，进一步明确和验证了其种属特性。同时，对经过初筛能结瘤的4株根瘤菌进行了生长曲线和生理生化特性的测定。本文结果表明在酸性土壤中，优势根瘤菌的地域差异性。

Tiger nut （Cyperus esculentus L., TN）, is a perennial herb of Cyperus Linn. It has the advantages of wide adaptability, short growth period, large biomass and high oil content. Stems and leaves of TN can be used as silage, and tubers of TN can be eaten raw or cooked. Noteworthily, TN can be used for oil production, and the cake can be further applied for feed. Therefore, as a new crop, TN integrates food, oil and fodder as a whole and has high economic value and development potential. In order to deeply understand TN and promote its high-value utilization, the research progress of the main nutrients such as oil, protein and polysaccharide in TN were summarizd. The physiological functions of TN in reducing blood lipid, lowering blood glucose, antioxidantion and protecting liver were expounded in detail. Moreover, the market scale and scientific research prospect were forecasted.

Intercropping patterns of soybean is an important model in southern China, the shading stress of high stalk crops is an important factor that affects the yield and quality of intercropped soybean. Therefore, it is particularly important to analyze the shade tolerance of soybean. In this study, a recombinant inbred lines （RILs） established from the shade-tolerant （Aijiaozao） and shade-sensitive （Chippewa）, 30 shade-tolerant and shade-sensitive accessions were selected to construct two DNA hybrid pools, respectively. Progeny hybrid and parent pools were covered for an average depth of 60× and 30×. By calculating the SNP-index of two progeny pools, the differences of SNP-index between shade-tolerant and shade-sensitive chromosomal segments were compared. And the candidate genes of shade tolerance were predicted based on their functional annotations. The results showed that 11 963 077 single nucleotide polymorphisms （SNPs） were obtained from four samples. There were 408 genes were found that they were mainly located on chromosome1 （51 942-505 708 bp）, chr. 4 （50 972 768-51 854 631 bp）, chr. 9 （48 778 767-50 178 743 bp） and chr. 18 （40 858 027-43 909 456 bp）. According to gene annotation and functional analysis, five candidate genes might be related to shade tolerance of soybean, including 1-aminocyclopropane-1-carboxylate oxidase 5-like, auxin-induced protein 5NG4-like, phytochrome-associated serine/threonine protein phosphatase-like, MYBJ6 and MYB128 were identified. They might play an important role in the process of soybean shade tolerance. The results will lay an important foundation for the molecular mechanism of soybean shade tolerance, and laid solid theoretical foundation for map-based cloning of shade-tolerant genes in soybean.

To better understand the resistance of Brassica napus to soil cadmium (Cd) stress, ABCC family genes and their transcriptional response to Cd stress in B. napus were studied. 38 ABCC homologs were identified, and were systematically analyzed on their phylogenetic evolution, chromosome location, gene structure and transcriptional response to Cd stress. Results revealed that all BnaABCC transporters fall into their corresponding clades of Arabidopsis thaliana, but ABCC9 was not found in B. napus. Most of the BnaABCCs were stable and hydrophobic, and Ka/Ks ratio was less than 1, suggested the results of strongly purification and selection. Gene structures of BnaABCCs were varied, which were disrupted by 6-34 introns. Vast of cis-acting elements could bind to their promoters, Dof might be the richest one. 38 BnaABCCs were mapped on chromosome 7 A and chromosome 8 C, and collinearity analysis showed abundant homologs in B. napus and Arabidopsis, B. rapa and B. oleracea. Transcriptome analysis showed that BnaABCCs were induced by Cd stress. Among them, BnaC1.ABCC3-1 and BnaA6.ABCC4-3 were identified as core genes in shoot and root respectively.

As rapeseed is the largest oil crop in China, rapeseed industry and its development are very impor? tant to secure Chinese oil supply. Although the industry had great achievements in recent years, comparative bene? fits declining and rapeseed import still led to seriously strokes. Rapeseed industry in China is facing a challenge as the major edible oil supplier for domestic market. Based on an extensive research on market and production of oil crop experts in 2018, we have been systematically figured the development status of China′s rapeseed industry by collecting first-hand data from both domestic and international situations. We also discussed the problems and sug? gested on promoting the industry to increase the edible oil security in China.

Sclerotinia stem rot of oilseed rape (Brassica napus) caused by fungal pathogen Sclerotinia sclerotiorum is one of the most devastating diseases worldwide. It is one of the most important biotic stressors affecting oilseed rape production. Breeding and cultivation of resistant varieties is the most efficient, economic and environmental friendly approach to control the disease. In this paper, research progress was reviewed in aspects of S. sclerotiorum pathogenesis, rapeseed resistance heredity, gene expression profiles responding to the disease infection and application of resistance genes in oilseed rape.