Abstract:

Soybean cyst nematode (SCN) is one of the most destructive pathogens in soybean production world? wide. Planting resistant cultivars is an environment friendly method to control SCN damage. Wild soybean, the wild relative of domestic soybean, is an invaluable gene pool to broaden the genetic background of soybean cultivars due to its higher genetic diversity and superior stress resistance. To date, lots of studies identified SCN resistance QTL using domestic soybeans, while only a few reports explored QTLs from wild soybeans. In this paper, SCN resistance QTL mapping was performed using specific-locus amplified fragment sequencing (SLAF-seq) with F2 and F2:3 popu? lations derived from a cross between Suinong 14 and ZYD03685. A high-density genetic linkage map comprising 7783 SLAF markers was constructed, with the total length of 2664.2 cM. Two QTLs of qSCN-1 and qSCN-2 were detected on Chromosome 18 (Chr18) 4.25-4.31 Mb, and Chr18 13.50-13.81 Mb, explaining 22.96% and 10.96% of resistance variation (female index) respectively. None of reported SCNresistance QTLs located at qSCN-2, indicat? ing that qSCN-2 was a novel QTL. In the region of qSCN-1 and qSCN-2, there were 6 and 14 genes included re? spectively. The QTLs and genes included in the QTL regions could not only shed light on the SCN resistance mecha? nisms, but also facilitate cultivar development employing wild soybean germplasm.

Key words:  , wild soybean；soybean cyst nematode；QTL；SLAF-seq