The objective of this study is to compare the photosynthetic characteristics of Glycine soja and variet⁃ies (strains) from Glycine soja × Glycine max cross under field conditions, to provide a theoretical basis for evaluat⁃ing breeding potential, and to provide high-efficiency parents for photosynthetic physiological breeding. All testedmaterials were planted in Chajintai Ranch in Tongliao City in 2017. Photosynthetic parameters of 15 tested materi⁃als were determined under natural conditions, and one-way ANOVA, correlation analysis, principal componentanalysis, cluster analysis and discriminant analysis were performed. The results showed that the photosynthetic pa⁃rameters of Glycine soja and varieties (strains) from Glycine soja × Glycine max cross showed significant differences.Pn was significantly positively correlated with CE, WUE and Tr, and significantly negatively correlated with Ci and SPAD. Five parameters for dividing photosynthetic capacity were selected, including Pn, CE, Tr, Ci and WUE. Clus⁃
ter analysis of Glycine soja and varieties (strains) from Glycine soja × Glycine max cross with 5 photosynthetic param⁃ eters were divided into 3 categories, and 3 discriminant models with high discriminative ability were established. By evaluated the photosynthetic characteristics, 10 varieties (strains) with high net photosynthetic rate and low intercel⁃ lular CO2 concentration, higher transpiration rate, high carboxylation efficiency were selected, including 600, 9010,9004, 0004, 9006, 0005, 9002, 9008, F-wild soybean hybrid S002 and 9014. These varieties (strains) also per⁃
formed well in yield and biomass.