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CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2020, Vol. 42 ›› Issue (6): 1035-.doi: 10.19802/j.issn.1007-9084.2019225

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Mechanism of 5-aminolevulinic acid on salt tolerance in peanut

  

  1. 1. Biotechnology Research Center of Shandong Academy of Agricultural Sciences, Jinan 250100, China;
    2. Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan 250100, China;
    3. Shandong Academy of Agricultural Sciences, Jinan 250100, China
  • Online:2020-12-28 Published:2020-11-18

Abstract:  Peanut (Arachis hypogaea L.) is an important oil and commercial crop worldwide. On the premise of
not competing for land with grain, utilization of saline-alkali land by peanut should be expanded, and the adverse ef⁃
fects of salt stress on peanut should be reduced. In this study, salt resistance mechanism of 5-aminolevulinic acid
(ALA) was determined by exogenous spraying under salt treatment. In the experiment, 200 mmol·L-1 NaCl was used
as salt stress treatment, and 6 concentration gradients of ALA as 0, 1, 10, 25, 50, 100 mg·L-1 were set. The results
showed that under salt stress, the activities of active oxygen scavenging enzymes in peanut leaves were significantly
increased after pretreatment with 10 mg·L-1 ALA, which could effectively remove active oxygen species in plants
and thus alleviate the damage of reactive oxygen species to peanut. In addition, the chlorophyll content in peanut
leaves increased, while malondialdehyde and proline content decreased. The expression of chlorophyll synthesis re⁃
lated genes CHLH, HEMA1 and CHLD was up-regulated by fluorescence quantitative PCR, which was consistent
with chlorophyll content. Under salt stress, exogenous ALA pretreatment could promote the expression of genes en⁃
coding important synthetase in chlorophyll synthesis pathway, thus increasing chlorophyll content and photosynthet⁃
ic capacity. It also could scavenge excessive active oxygen in cells, and activate Ca2+/CaM signal pathway, further to
improve the antioxidant capacity of peanut plants and alleviate the damage of salt stress on peanut.

Key words: peanut, 5-aminolevulinic acid(ALA), salt stress, chlorophyll synthesis

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