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CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2020, Vol. 42 ›› Issue (4): 623-.doi: 10.19802/j.issn.1007-9084.2019296

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Screening and differential expression of drought related genes in carbohydrate metabolism pathway basen transcriptome sequencing in wild soybean

  

  1. 1. College of Agronomy and Biotechnology, Hebei Normal University of Science and Technology, Changli 066600,
    China; 2. Department of Physics, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China
  • Online:2020-08-28 Published:2020-09-04

Abstract: Compared with soybean, wild soybean had wider genetic diversity, it was important to screen the
drought-resistant genes by analyzing the gene expression patterns at the meta-omics level. High-throughput se⁃
quencing technique was applied to RNA sequences at different time treated with PEG6000 in wild soybean grown to
30d, and 1796 carbohydrate metabolism pathway related sequences were obtained. Starch and sucrose metabolic
pathways had the most differentially expressed genes, followed by galactose metabolic pathway. The differentially
expressed genes were most expressed at 12 h of drought stress. A protein interaction network was constructed for the
above 109 differentially expressed genes. Eight key genes were obtained by taking >10 node degree in 109 DEGs. It
provides a theoretical basis for further research on carbohydrate metabolism related genes in wild soybean under
drought stress.

Key words:  , wild soybean;RNA-seq;drought stress;glycometabolism

CLC Number: