Abstract: In order to identify stable and universal QTLs for the number of four-seed-pod trait on soybean in different years and groups, based on the phenotypic data in 2013-2016 year, a RILs population and its SLAF-seq high density genetic map were used to identify the QTLs. Phenotypic validation had been done with introgression lines carrying the target interval. The results showed that there were 8 QTLs in two chromosome identified. Four QTLs in Gm06 chromosome had close position and positive additive effect. The target interval size was 0.62Mb. Four QTLs in Gm16 chromosome had same negative and positive additive effect. The target interval size was 1.04Mb. These QTLs were stable in different years. 5 introgression lines carrying the target interval of Gm06 chro⁃ mosome had significantly higher phenotypic value than recurrent parent in different years. 7 introgression lines car⁃ rying the target interval of Gm16 chromosome had significantly lower phenotypic value than recurrent parent in dif⁃ ferent years. The results showed that the target interval introgression led to synergistic or deleterious effects of the phenotypic of the number of four-seed-pod trait. The accuracy and commonality of QTLs were verified in genomewide introgression lines. The results of this research provided theoretical foundation for candidate gene mining and molecular assisted breeding of the number of four-seed-pod trait on soybean. 

Key words: color:#000000, font-family:", sans serif", ,tahoma,verdana,helvetica, font-size:12px, font-style:normal, font-weight:normal, line-height:1.5, text-decoration:none, "> , soybean；the number of four-seed-pod；QTL identification；interactive group validation ,