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CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2020, Vol. 42 ›› Issue (3): 356-.doi: 10.19802/j.issn.1007-9084.2020017

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Quantification of major triglycerides in vegetable oils by liquid chromatography-tandem mass spectrometry

  

  1. 1. Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China; 2. Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China; 3. Laboratory of Quality and Safety Risk Assessment for Oilseed Products (Wuhan), Ministry of Agriculture and Rural Affairs, Wuhan 430062, China; 4. Quality Inspection and Test Center for Oilseed Products, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China; 5. Henan University of Technology, Zhengzhou 450001, China
  • Online:2020-06-28 Published:2020-06-09
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Abstract:

There are many types of triglycerides in vegetable oils, and their structures are complex, which is difficult to quantify with certainty. This paper used liquid chromatography-tandem mass spectrometry, selective re⁃ action monitoring (SRM) mode, two pairs of ion pairs for qualitative, external standard method to accurately quantify seven major triglycerides in vegetable oils, including: glyceryl trilinolein (LLL), 1-oleic acid-2,3-linoleic acid glyc⁃ eride (OLL), 1-palmitic acid-2,3-linoleic acid glyceride (PLL), 1-palmitic acid-2-oleic acid-3-linoleic acid glyc⁃eride (POL), glyceryl trioleate (OOO), 1,2-oleate-3-glycerate glyceride(OOP), 1-palmitic acid-2-oleic acid-3-stearic acid glyceride(POS). The triglycerides were separated by reversed-phase chromatography, and the isopropylalcohol / acetonitrile mixed solution and acetonitrile / water mixed solution were used as mobile phase gradient elu⁃tion. The triglyceride could be separated and detected within 15 minutes. This method has a good linear relation⁃ship, all above 0.996, with a detection limit of 0.3 μg/kg, a limit of quantification of 1.0 μg/kg, and a precision(RSD) of 1.2% to 4.9%. The method is simple and fast to operate, and it can be used to detect the seven triglycer⁃ides in vegetable oils such as rapeseed oil, corn oil, and peanut oil.

Key words:  , vegetable oils;triglycerides;liquid chromatography-tandem mass spectrometry;accurate quanti?fication

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