Abstract: Nitrogen is one of the many elements necessary for plant growth. Symbiosis of soybean rhizobium can provide sufficient nitrogen for soybean. It is important to study the mechanism of soybean rhizobium symbiosis and excavate candidate genes for controlling symbiosis. Given that the QTL for regulating soybean symbiosis is large at this stage, it cannot be directly applied to practice. In this experiment, 68 QTLs for soybean nodulation were col⁃ lated, and they were optimized by Overview and colinearity analysis. A high-confidence QTLs interval was obtained on the chromosome Gm06. The gene was annotated and 43 genes were obtained, including one coding system C2 Calcium / lipid binding and GRAM domain-containing proteins, a side root-forming protein and an E3 ubiquitin li⁃ gase-related protein, and it’s a so possible to search for strains with inserts at this location in the CSSLs population for identification of nodulation traits. The results showed that 15-15.5 Mb on the C2 linkage group was crucial for the symbiotic nodulation of soybean rhizobium.

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