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CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2020, Vol. 42 ›› Issue (1): 17-.doi: 10.19802/j.issn.1007-9084.2020023

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 Construction of TtsI Mutant of Rhizobia and Identification of Nodulation Phenotype

  

  1. Laboratory of Soybean Genetic Improvement, Northeast Agricultural University, Harbin, Heilongjiang 150030, China
  • Online:2020-02-28 Published:2020-03-03

Abstract: In this study, TtsI, a regulator of HH103 type III secretion system in Sinorhizobium was detected. The mutant HH103ΩTtsI was obtained by three-parental hybridization and identified by PCR. Type III effector ex⁃ pression analysis showed that TtsI mutation could inhibit the transcription and expression of effector genes such as NopAA, NopD, NopL, and NopP in the presence of genistein. When TtsI mutated, it could significantly inhibit the synthesis of rhizobium HH103 type III effector. TtsI mutants and wild-type rhizobium HH103 were used to identify nodules in 10 soybean germplasm of different ecological regions. These results showed that the TtsI mutation could significantly reduce the average number of nodule and the dry weight of nodule. The nodule phenotype identification results of the introduced line population showed that the TtsI mutant significantly reduced the average nodule num⁃ ber of the introduced line. This showed that TtsI played an important role in the establishment of a soybean-rhizobia symbiotic system depending on other type III effectors.

Key words: color:#000000, font-family:", sans serif", ,tahoma,verdana,helvetica, font-size:12px, font-style:normal, font-weight:normal, line-height:1.5, text-decoration:none, ">soybean;Sinorhizobium fredii HH103;type III effector;TtsI

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