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CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2021, Vol. 43 ›› Issue (5): 771-.doi: 10.19802/j.issn.1007-9084.2020057

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Constructing and comparing of SSR map on introgressed segment linkaged with Rfo gene for Ogu CMS restorer line 16C

  

  • Online:2021-11-01 Published:2021-11-01

Abstract: To push the application of Ogu CMS restorer line 16C, SSR map of introgressed segment linkaged with Rfo gene of the Ogu CMS restorer line 16C was constructed. And comparison of 16C with R2000 from Europe was carried out by SSR molecular makers. Sterile line 81A and the restorer line 16C were used as parents to develop an F2 population. 38 SSR primers originated from radish reference genome were designed to screen SSR markers linked with Rfo gene in F2. Results showed 12 of the primers linking with Rfo gene. Among them, 2 markers, R9SSR2416 and R9SSR3326, were at the two end sides of introgressed fragment separately. 11 of the 38 primers were found to be different between 16C and R2000 by PCR. 2 of the 11 markers were specific for 16C and the other 9 were specific for R2000. The introgressed segment in 16C was about 3.30 Mb (Chromosome_R9:8480586-11779992). Thus it was different from R2000 on both size and source.

Key words: Brassica napus, Ogu CMS restorer, SSR marker, map of molecular marker

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