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CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2021, Vol. 43 ›› Issue (4): 627-.doi: 10.19802/j.issn.1007-9084.2020079

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Exploration of EMS mutagenesis system and mutants screening of peanut

  

  1. Shandong Peanut Research Institute, Qingdao 266100,China
  • Online:2021-08-28 Published:2021-08-30

Abstract:

The creation of mutants is of great significance to genetic improvement and functional genomics of peanut. In order to obtain new peanut mutant germplasm, this study explored EMS mutagenesis conditions of peanut varieties with different genotypes. It was found that the appropriate mutagenic concentrations of Huayu 22, Huayu 71, Huayu 9301, Shitouqi and Fuhuasheng were 0.4%, 0.5%, 0.3%, 0.5% and 0.3%, respectively. The analysis of bud length, plant height, root length and root number in seedling stage under the optimum concentration showed that all the 5 cultivars were able to form seedlings normally, but all were seriously damaged. On the basis of the preliminary experiment, 5000 Huayu 22 seed swas treated by 0.4% EMS solution. Both M1 and M2 generation mutants showed diversified phenotypes. For example, plant height, plant type, pod, seed kernel, branching number and leaf shape were changed. The phenotypic variation rate was 12.9%. Our work provides abundant germplasm for peanut genetics research and breeding.

Key words: peanuts, EMS mutagenesis, mutagenesis condition, mutants

CLC Number: