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CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2021, Vol. 43 ›› Issue (5): 743-.doi: 10.19802/j.issn.1007-9084.2020090

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Thaumatin-like protein kinase BnTLK1 from Brassica napus related to fungal disease resistance

  

  1. Key Laboratory of Biology and Genetic Improvement of Oil Crops,the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China
  • Online:2021-11-01 Published:2021-11-01

Abstract: As the fifth family of pathogenesis-related proteins, TLP (thaumatin-like protein) plays an important defensive role in the interaction between plant and pathogen. In some species, the C-terminal of TLP fused kinase domain, but the fusion gene function required to be discussed. In Brassica napus, we identified and cloned a gene named BnTLK1, which contained a TLP domain at N termini and serine/ threonine-rich receptor-like kinase domain at C termini. Sequence characterization showed that BnTLK1 had typical transmembrane structure and formed two independent units at 3D structure level. The expression of BnTLK1 was low in different tissues, high expressed in the cultivar 'ZY821' of high resistance to Sclerotinia sclerotiorum, and induced by S. sclerotiorum based on public transcriptome data analysis. To investigate the effect of BnTLK1 on S. sclerotiorum and other fungus, BnTLK1 were expressed in prokaryotic system and get the soluble protein BnTLK1 after overnight induction at 0.4 mmol/L IPTG and 17℃. Bacteriostatic experiment further confirmed BnTLK1 could indeed inhibit the mycelium growth of Sclerotinia sclerotiorum and Botrytis cinerea.

Key words: Brassica napus, thaumatin-like protein, kinase, Sclerotinia sclerotiorum, prokaryotic expression, antibacterial activity

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