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CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2021, Vol. 43 ›› Issue (5): 752-.doi: 10.19802/j.issn.1007-9084.2020109

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BnTLP1 on resistance to Sclerotinia sclerotiorum in Brassica napus

  

  1. 1. Oil Crops Research Institute of Chinese Academy of Agriculture Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China; 2. Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University,Wuhan 430062, China
  • Online:2021-11-01 Published:2021-11-01

Abstract: BnTLP1 gene was previously found highly expressed in tolerant Brassica napus cv. Zhongyou 821. To deep understand the tolerance to Sclerotinia sclerotiorum, BnTLP1 gene was identified using overexpressed transgenic Arabidopsis lines. Leaf inoculation was carried out using BnTLP1 overexpression transgenic Arabidopsis lines, wild-type and tlp1-1 mutant by S. sclerotiorum mycelial agar plugs. Compared to WT, BnTLP1 overexpression lines exhibited the smallest lesion, while tlp1-1 showed the largest one, suggesting that BnTLP1 could positively regulated Sclerotinia disease resistance. Subcellular localization indicated that BnTLP1 was located in cell membrane and nucleus. Expression analysis of marker genes in different defense signaling showed that jasmonic acid/ethylene might play roles in the early stage of BnTLP1-mediated diseased resistance.

Key words: Brassica napus, BnTLP1 gene, Sclerotinia sclerotiorum, transgenic Arabidopsis, subcellular localization, asmonic acid/ethylene pathway

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