Verifying high variation regions based on sect. Arachis chloroplast genome and revealing the interspecies genetic relationship

doi:10.19802/j.issn.1007-9084.2020120

Abstract

Abstract:

Cultivated peanut (Arachis hypogaea L.) is one of the most important oilseed crops and cash cropsworldwide. However, the genetic improvement of peanut cultivar is restricted by its narrow genetic base. The highly variable wild peanut species may serve as a rich genetic source of disease and insect resistant genetic resources. In order to take advantage of these wild peanut resources, the phylogenetic relationship of the Arachis species need firstly to be understood. The whole chloroplast (cp) genomes with uniparental inheritance and low order classification will help to reveal the sect. Arachis genetic relationship, but more time and procedures would be reqrired. Here,the total of 9 candidate high variation regions was targeted based on the available 15 sect. Arachis cp complete genomes and 1 sect. Erectoides cp complete genome. By PCR verification, the region psbE-petL was found to be the highest variation region, which was used to detect the genetic relationship rapidly for the unknown sect. Arachis species. By comparing the topological structure of the phylogenetic tree, our study provided important reference for new species delimitation and interspecies exploration.

Key words: sect. Arachis；chloroplast genomes, candidate high variation regions；genetic relationship

CLC Number:

中图分类号：S565.2

WANG Juan, LI Chun-juan, SHI Da-chuan, LIU Yu, TANG Rong-hua, HE Liang-qiong, ZHAO Xiao-bo, YUAN Cui-ling, SUN Quan-xi, YAN Cai-xia, SHAN Shi-hua. Verifying high variation regions based on sect. Arachis chloroplast genome and revealing the interspecies genetic relationship[J]. CHINESE JOURNAL OF OIL CROP SCIENCES, 2021, 43(3): 495-.

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