CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2021, Vol. 43 ›› Issue (3): 418-.doi: 10.19802/j.issn.1007-9084.2020132

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Breeding for thermo-sensitive pol cytoplasmic male sterile line and its restorer with high oleic acid through molecular marker-assisted selection in Brassica napus


  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China
  • Online:2021-06-28 Published:2021-07-01


      High oleic rapeseed (Brassica napus L.) oil has high nutrition with good quality for cooking, processing and storage. For high oleic acid breeding and heterosis utilization, male sterile line and restoring line were bred  by using line J-3111 with high oleic acid as donor parent. Its high oleic allele (fad2 gene) was succefully transferred into a thermo-sensitive pol cytoplasmic male sterile (pol TCMS) line 616A and a restoring line L-135R by backcross and molecular marker-assisted selection. Results showed that oleic content of the improved strains was up to 75.18%. Meanwhile, yield traits of the improved lines showed no significant difference comparing with recipient parents in silique length, seeds number per silique and thousand-seeds weight. Based on whole-genome resequencing data, InDel markers were developed closely-linked with fad2 gene. Thus we obtained improved pol TCMS line and restoring line with high oleic contents, which laid foundation for breeding of high-yield and high-oleic cotent.

Key words:  , Brassica napus L.;backcross breeding;molecular marker-assisted selection;pol TCMS;high oleic acid

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