Breeding of a high oil-content restorer line 4061R for dominant genic male sterile in rapeseed

doi:10.19802/j.issn.1007-9084.2020138

Abstract

Abstract: To breed high oil and double-low （low erucic acid and low glucosinolates） restorer with morphological marker for dominant genic male sterile in rapeseed, breeding materials with lobed-leaf, high oil content or restoring gene were utilized as parents. The restorer line 4061R with lobed-leaf, high oil-content and precocious feature was obtained through composite-crossing combined with directional selection. The field test and quality analysis showed that 4061R had lobed leaves with total growth period of 210 d, plant height of 156.2 cm, seed number per siliqua of 20.1, silique numbers of per plant of 300.2, 1000-seed weight of ～4.3 g. In Shanghai, yield of 2219.7 kg/hm2 was obtained under 18.5×104 plants/hm2. The restorer seed oil-content was 50.6%, with erucic acid 0.7%, and glucosinolate 19.6 μmol/g detected by near-infrared reflectance spectroscopy. Thus 4061R was an ideal restorer line with better comprehensive properties.

Key words: rapeseed, dominant genic male sterile restorer, lobed leaf, oil-content

CLC Number:

中图分类号：S565.4

ZHU Ji-feng, ZHANG Jun-ying, JIANG Mei-yan, JIANG Jian-xia, YANG Li-yong, LI Yan-li, WANG Wei-rong, ZHOU Xi-rong. Breeding of a high oil-content restorer line 4061R for dominant genic male sterile in rapeseed[J]. CHINESE JOURNAL OF OIL CROP SCIENCES, 2021, 43(5): 778-.

References

［1］李树林，周志疆，周熙荣. 油菜显性核不育三系法制种［J］. 上海农业学报， 1995， 11（1）： 21-26.
［2］宋来强，傅廷栋，杨光圣，等. 甘蓝型油菜显性核不育基因及与恢复基因的等位性分析［J］. 中国农业科学， 2006， 39（3）： 456-462. DOI：10.3321/j.issn： 0578-1752.2006.03.004.
［3］ Xin Q， Shen Y， Li X， et al. MS5 mediates early meiotic progression and its natural variants may have applications for hybrid production in Brassica napus［J］. Plant Cell， 2016， 28（6）： 1263-1278. DOI：10.1105/tpc.15.01018.
［4］ Sernyk J L， Stefansson B R. Heterosis IN summer rape （Brassica napus l.）［J］. Can J Plant Sci， 1983， 63（2）： 407-413. DOI：10.4141/cjps83-046.
［5］谭祖猛，李云昌，胡琼，等. 通过分子标记估算遗传距离预测甘蓝型油菜的杂种优势［J］. 中国油料作物学报， 2007， 29（2）： 126-132. DOI：10.3321/j.issn： 1007-9084.2007.02.004.
［6］ Ni X Y， Huang J X， Ali B， et al. Genetic analysis and fine mapping of the LOBED-LEAF 1 （BnLL1） gene in rapeseed （Brassica napus L.）［J］. Euphytica， 2015， 204（1）： 29-38. DOI：10.1007/s10681-014-1319-6.
［7］ Hu L M， Zhang H， Yang Q Y， et al. Promoter variations in a homeobox gene， BnA10.LMI1， determine lobed leaves in rapeseed （Brassica napus L.）［J］. Theor Appl Genet， 2018， 131（12）： 2699-2708. DOI：10.1007/s00122-018-3184-5.
［8］郎丽娜，赵娜，马兵，等. 甘蓝型油菜花叶性状的遗传及基因定位［J］. 华北农学报， 2016， 31（4）： 39-43. DOI：10.7668/hbnxb.2016.04.007.
［9］浦惠明，傅寿仲，戚存扣，等. 花叶性状的遗传及其在杂交油菜育种中的应用［J］. 中国油料作物学报， 2001， 23（3）： 60-62. DOI：10.3321/j.issn： 1007-9084.2001.03.018.
［10］ Kaur B， Sangha M K， Kaur G. Development of near-infrared reflectance spectroscopy （NIRS） calibration model for estimation of oil content in Brassica juncea and Brassica napus［J］. Food Anal Methods， 2017， 10（1）： 227-233. DOI：10.1007/s12161-016-0572-9.
［11］张俊英，蒋美艳，李延莉，等. 甘蓝型显性核不育双低油菜新品种‘核杂17号’的选育［J］. 上海农业学报， 2017， 33（5）： 13-16. DOI：10.15955/j.issn1000-3924.2017.05.03.
［12］周熙荣，庄静，孙超才，等. 甘蓝型油菜显性核不育双低杂交种核杂7号的选育［J］. 上海农业学报， 2006， 22（4）： 6-9. DOI：10.3969/j.issn.1000-3924.2006.04.002.
［13］蒋美艳，孙超才，杨立勇，等. 甘蓝型油菜显性核不育恢复系“HF06”的选育［J］. 上海交通大学学报（农业科学版）， 2013， 31（5）： 26-28， 60. DOI：10.3969/J.ISSN.1671-9964.2013.05.006.
［14］李殿荣，陈文杰，于修烛，等. 双低菜籽油的保健作用与高含油量优质油菜育种及高效益思考［J］. 中国油料作物学报， 2016， 38：850-854. DOI：10.7505/j.issn.1007-9084.2016.06.021.
［15］朱吉风，张俊英，蒋美艳，等. 中国冬油菜育成品种产量和主要性状变化趋势［J］. 上海农业学报， 2020， 36（2）： 13-18. DOI：10.15955/j.issn1000-3924.2020.02.03.

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