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CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2020, Vol. 42 ›› Issue (4): 603-.doi: 10.19802/j.issn.1007-9084.2020159

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Genes differentially expression in rapeseed hybrid Ningza 21 and its parents at seedling stage under salt tolerance

  

  1. 1. Oil Crops Research Institute of Chinese Academy of Agricultural Sciences / Key Laboratory of Biology and Genetic
    Improvement of Oil Crops; Ministry of Agriculture and Rural Affairs, Wuhan 430062, China; 2. Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences; Nanjing Sub-center, National Center of Oil Crops Improvement; Key Laboratory of Cotton and Rapeseed (Nanjing), Ministry of Agriculture and Rural Affairs, Nanjing 210014, China; 3.Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 4. Institute of Life Sciences, Jiangsu University, Zhenjiang 212023, China
  • Online:2020-08-28 Published:2020-09-04

Abstract: To understand rapeseed(Brassica napus L.)heterosis on salt tolerance, hybrid cultivar Ningza 21
was used to investigate genes differentially expression compared with its parental lines. Through the next-genera⁃
tion high-throughput transcriptome sequencing technology with no salt treatments as negative control, 1078 differ⁃
entially expressed genes (DEGs) were obtained, which were recognized to be closely related to heterosis of salt toler⁃
ance in oilseed rape at early seedling stage. These DEGs were annotated by released salt stress related QTLs, and
expression of some DEGs were verified by qPCR, indicating the reliability of these DEGs. Additionally, the hybrid
had more up-regulation candidate DEGs in shoot than those in root, and the expression pattern of DEGs was much
closer to that of the maternal line than the paternal line in hybrid Ningza 21. More DEGs in hybrid similar to female
similar to male parent were much related to redox and other regulatory functions. It was predicted that SNP loci were
specific for heterosis of salt tolerance. All the results were expected to be helpful for salt tolerance improvement of
hybrid rapeseed.

Key words:  , Brassica napus L.;salt tolerance;heterosis;transcriptome;genetic mechanism

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