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CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2021, Vol. 43 ›› Issue (5): 816-.doi: 10.19802/j.issn.1007-9084.2020183

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Sequence and expression analysis of DREB transcription factor gene AhDREB3 in peanut under abiotic stress

  

  1. 1. Shandong Peanut Research Institute, Qingdao 266100, China; 2. Binzhou Academy of Agricultural Sciences, Binzhou 256600, China; 3. Yantai Agricultural Technology Extension Center, Yantai 264001, China; 4. Shandong Wolong Seed Industry Co., Yinan 276301, China; 5. Yinan Agricultural And Rural Bureau of Yinan County, Yinan 276300, China
  • Online:2021-11-01 Published:2021-11-01

Abstract: DREB belongs to an important subfamily of AP2/ERF transcription factor families, and have important functions in regulation of plant abiotic stress resistance. To screen the potential function of stress-related gene AhDREB3 in peanut, full-length sequence of the drought-responsive element-binding protein 3 (AhDREB3) was focused. Evolution of its protein encoded by AhDREB3 was analyzed. Primers were designed to detect its expression under several abiotic stresses by fluorescence quantitative PCR. Results showed that AhDREB3 gene did not respond to low temperature in leaves and roots of peanut, but responded significantly to high salt (in leaves and roots) and drought (in roots) stress. In addition, the AhDREB3 gene did not respond significantly to exogenous ABA in peanut leaves and roots, suggesting that the gene might play a role in peanut through an ABA-independent pathway.

Key words: peanut, DREB transcription factor, expression analysis, abiotic stresses

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