Sequence and expression analysis of DREB transcription factor gene AhDREB3 in peanut under abiotic stress

doi:10.19802/j.issn.1007-9084.2020183

Abstract

Abstract: DREB belongs to an important subfamily of AP2/ERF transcription factor families, and have important functions in regulation of plant abiotic stress resistance. To screen the potential function of stress-related gene AhDREB3 in peanut, full-length sequence of the drought-responsive element-binding protein 3 (AhDREB3) was focused. Evolution of its protein encoded by AhDREB3 was analyzed. Primers were designed to detect its expression under several abiotic stresses by fluorescence quantitative PCR. Results showed that AhDREB3 gene did not respond to low temperature in leaves and roots of peanut, but responded significantly to high salt (in leaves and roots) and drought (in roots) stress. In addition, the AhDREB3 gene did not respond significantly to exogenous ABA in peanut leaves and roots, suggesting that the gene might play a role in peanut through an ABA-independent pathway.

Key words: peanut, DREB transcription factor, expression analysis, abiotic stresses

CLC Number:

中图分类号：S565.2

CHEN Na, PAN Li-juan, CHEN Ming-na, WANG Tong, XU Jing, WANG Zi-qiang, YANG Zhen, XIE Hong-feng, ZOU Zong-feng, HUANG Xiang, HUANG Hui-wen, YU Shan-lin, CHI Xiao-yuan. Sequence and expression analysis of DREB transcription factor gene AhDREB3 in peanut under abiotic stress[J]. CHINESE JOURNAL OF OIL CROP SCIENCES, 2021, 43(5): 816-.

References

［1］ Agarwal P K， Shukla P S， Gupta K， et al. Bioengineering for salinity tolerance in plants： state of the art［J］. Mol Biotechnol， 2013， 54（1）： 102-123. DOI：10.1007/s12033-012-9538-3.
［2］ Nakano T， Suzuki K， Fujimura T， et al. Genome-wide analysis of the ERF gene family in Arabidopsis and rice［J］. Plant Physiol， 2006， 140（2）： 411-432. DOI：10.1104/pp.105.073783.
［3］ Sakuma Y， Liu Q， Dubouzet J G， et al. DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs， transcription factors involved in dehydration- and cold-inducible gene expression［J］. Biochem Biophys Res Commun， 2002， 290（3）： 998-1009. DOI：10.1006/bbrc.2001.6299.
［4］ Licausi F， Giorgi F M， Zenoni S， et al. Genomic and transcriptomic analysis of the AP2/ERF superfamily in Vitis vinifera［J］. BMC Genomics， 2010， 11： 719. DOI：10.1186/1471-2164-11-719.
［5］ Rashid M， He G， Yang G， et al. AP2/ERF transcription factor in rice： genome-wide canvas and syntenic relationships between monocots and eudicots［J］. Evol Bioinform Online， 2012， 8： 321-355. DOI：10.4137/ebo.s9369.
［6］ Xie Z L， Nolan T M， Jiang H， et al. AP2/ERF transcription factor regulatory networks in hormone and abiotic stress responses in Arabidopsis［J］. Front Plant Sci， 2019， 10： 228. DOI：10.3389/fpls.2019.00228.
［8］ Liu Q， Kasuga M， Sakuma Y， et al. Two transcription factors， DREB1 and DREB2， with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression， respectively， in Arabidopsis［J］. Plant Cell， 1998， 10（8）： 1391-1406. DOI：10.1105/tpc.10.8.1391.
［9］ Gilmour S J， Zarka D G， Stockinger E J， et al. Low temperature regulation of the Arabidopsis CBF family of AP2 transcriptional activators as an early step in cold-induced COR gene expression［J］. Plant J， 1998， 16（4）： 433-442. DOI：10.1046/j.1365-313x.1998.00310.x.
［10］ Shinwari Z K， Nakashima K， Miura S， et al. An Arabidopsis gene family encoding DRE/CRT binding proteins involved in low-temperature-responsive gene expression［J］. Biochem Biophys Res Commun， 1998， 250（1）： 161-170. DOI：10.1006/bbrc.1998.9267.
［11］ Dubouzet J G， Sakuma Y， Ito Y， et al. OsDREB genes in rice， Oryza sativa L.， encode transcription activators that function in drought-， high-salt- and cold-responsive gene expression［J］. Plant J， 2003， 33（4）： 751-763. DOI：10.1046/j.1365-313x.2003.01661.x.
［12］ Jaglo-Ottosen K R. Arabidopsis CBF1 overexpression induces COR genes and enhances freezing tolerance［J］. Science， 1998， 280 （5360）： 104-106. DOI：10.1126/science.280.5360.104.
［13］ Wang Q Y， Guan Y C， Wu Y R， et al. Overexpression of a rice OsDREB1F gene increases salt， drought， and low temperature tolerance in both Arabidopsis and rice［J］. Plant Mol Biol， 2008， 67（6）： 589-602. DOI：10.1007/s11103-008-9340-6.
［14］ Zhang Y， Chen C， Jin X F， et al. Expression of a rice DREB1 gene， OsDREB1D， enhances cold and high-salt tolerance in transgenic Arabidopsis［J］. BMB Rep， 2009， 42（8）： 486-492. DOI：10.5483/bmbrep.2009.42.8.486.
［15］ Shen Y G， Zhang W K， He S J， et al. An EREBP/AP2-type protein in Triticum aestivum was a DRE-binding transcription factor induced by cold， dehydration and ABA stress［J］. Theor Appl Genet， 2003， 106（5）： 923-930. DOI：10.1007/s00122-002-1131-x.
［16］ Yamasaki Y， Randall S K. Functionality of soybean CBF/DREB1 transcription factors［J］. Plant Sci， 2016， 246： 80-90. DOI：10.1016/j.plantsci.2016.02.007.
［17］ Matsukura S， Mizoi J， Yoshida T， et al. Comprehensive analysis of rice DREB2-type genes that encode transcription factors involved in the expression of abiotic stress-responsive genes［J］. Mol Genet Genom， 2010， 283（2）： 185-196. DOI：10.1007/s00438-009-0506-y.
［18］ Egawa C， Kobayashi F， Ishibashi M， et al. Differential regulation of transcript accumulation and alternative splicing of a DREB2 homolog under abiotic stress conditions in common wheat［J］. Genes Genet Syst， 2006， 81（2）： 77-91. DOI：10.1266/ggs.81.77.
［19］ Sakuma Y， Maruyama K， Qin F， et al. Dual function of an Arabidopsis transcription factor DREB2A in water-stress-responsive and heat-stress-responsive gene expression［J］. PNAS， 2006， 103（49）： 18822-18827. DOI：10.1073/pnas.0605639103.
［20］ Nakashima K， Shinwari Z K， Sakuma Y， et al. Organization and expression of two Arabidopsis DREB2 genes encoding DRE-binding proteins involved in dehydration- and high-salinity-responsive gene expression［J］. Plant Mol Biol， 2000， 42（4）： 657-665. DOI：10.1023/a： 1006321900483.
［21］ Wang C T， Yang Q， Wang C T. Isolation and functional characterization of ZmDBP2 encoding a dehydration-responsive element-binding protein in Zea mays［J］. Plant Mol Biol Report， 2011， 29（1）： 60-68. DOI：10.1007/s11105-010-0210-4.
［22］ Abogadallah G M， Nada R M， Malinowski R， et al. Overexpression of HARDY， an AP2/ERF gene from Arabidopsis， improves drought and salt tolerance by reducing transpiration and sodium uptake in transgenic Trifolium alexandrinum L.［J］. Planta， 2011， 233（6）： 1265-1276. DOI：10.1007/s00425-011-1382-3.
［23］ Liu N， Zhong N Q， Wang G L， et al. Cloning and functional characterization of PpDBF1 gene encoding a DRE-binding transcription factor from Physcomitrella patens［J］. Planta， 2007， 226（4）： 827-838. DOI：10.1007/s00425-007-0529-8.
［24］ Ren M Y， Wang Z L， Xue M， et al. Constitutive expression of an A-5 subgroup member in the DREB transcription factor subfamily from Ammopiptanthus mongolicus enhanced abiotic stress tolerance and anthocyanin accumulation in transgenic Arabidopsis［J］. PLoS One， 2019， 14（10）： e0224296. DOI：10.1371/journal.pone.0224296.
［25］ Lin R C， Park H J， Wang H Y. Role of Arabidopsis RAP2.4 in regulating light- and ethylene-mediated developmental processes and drought stress tolerance［J］. Mol Plant， 2008， 1（1）： 42-57. DOI：10.1093/mp/ssm004.
［26］ Rae L， Lao N T， Kavanagh T A. Regulation of multiple aquaporin genes in Arabidopsis by a pair of recently duplicated DREB transcription factors［J］. Planta， 2011， 234（3）： 429-444. DOI：10.1007/s00425-011-1414-z.
［27］ Wan X R， Mo A Q， Liu S， et al. Constitutive expression of a peanut ubiquitin-conjugating enzyme gene in Arabidopsis confers improved water-stress tolerance through regulation of stress-responsive gene expression［J］. J Biosci Bioeng， 2011， 111（4）： 478-484. DOI：10.1016/j.jbiosc.2010.11.021.
［28］ Wang T， Chen X， Zhu F， et al. Characterization of peanut germin-like proteins， AhGLPs in plant development and defense［J］. PLoS One， 2013， 8（4）： e61722. DOI：10.1371/journal.pone.0061722.
［29］ Pruthvi V， Narasimhan R， Nataraja K N. Simultaneous expression of abiotic stress responsive transcription factors， AtDREB2A， AtHB7 and AtABF3 improves salinity and drought tolerance in peanut （Arachis hypogaea L.）［J］. PLoS One， 2014， 9（12）： e111152. DOI：10.1371/journal.pone.0111152.
［30］ Sarkar T， Thankappan R， Kumar A， et al. Heterologous expression of the AtDREB1A gene in transgenic peanut-conferred tolerance to drought and salinity stresses［J］. PLoS One， 2014， 9（12）： e110507. DOI：10.1371/journal.pone.0110507.
［31］ Wan L Y， Wu Y S， Huang J Q， et al. Identification of ERF genes in peanuts and functional analysis of AhERF008 and AhERF019 in abiotic stress response［J］. Funct Integr Genom， 2014， 14（3）： 467-477. DOI：10.1007/s10142-014-0381-4.
［32］ Zhang J C， Chen N， Zhang Z M， et al. Peanut ethylene-responsive element binding factor （AhERF6） improves cold and salt tolerance in Arabidopsis［J］. Acta Physiol Plant， 2016， 38（7）： 1-10. DOI：10.1007/s11738-016-2201-z.
［33］万书波. 中国花生栽培学［M］. 上海：上海科学技术出版社， 2003.
［34］胡晓辉，孙令强，苗华荣，等. 不同盐浓度对花生品种耐盐性鉴定指标的影响［J］. 山东农业科学， 2011， 43（11）： 35-37. DOI：10.14083/j.issn.1001-4942.2011.11.022.
［35］ Zhuang W， Chen H， Yang M， et al. The genome of cultivated peanut provides insight into legume karyotypes， polyploid evolution and crop domestication［J］. Nat Genet， 2019， 51（5）： 865-876. DOI：10.1038/s41588-019-0402-2.
［36］ Riechmann J L， Heard J， Martin G， et al. Arabidopsis transcription factors： genome-wide comparative analysis among eukaryotes［J］. Science， 2000， 290（5499）： 2105-2110. DOI：10.1126/science.290.5499.2105.
［37］汤珣，官亚琳，陈玲玲，等. 香鳞毛蕨DfDREB基因克隆与表达模式分析［J］. 西北植物学报， 2020， 40（7）： 1105-1113. DOI：10.7606/j.issn.1000-4025.2020.07.1105.
［38］陈红，化烨，王正旭，等. 北方旱地红小豆DREB2A基因克隆及表达分析［J］. 分子植物育种， 2020， 18（12）： 3825-3831. DOI：10.13271/j.mpb.018.003825.
［39］ Mizoi J， Shinozaki K， Yamaguchi-Shinozaki K. AP2/ERF family transcription factors in plant abiotic stress responses［J］. Biochim Biophys Acta， 2012， 1819（2）： 86-96. DOI：10.1016/j.bbagrm.2011.08.004.
［40］ Phukan U J， Jeena G S， Tripathi V， et al. Regulation of Apetala2/ethylene response factors in plants［J］. Front Plant Sci， 2017， 8： 150. DOI：10.3389/fpls.2017.00150.
［41］ Gu C， Guo Z H， Hao P P， et al. Multiple regulatory roles of AP2/ERF transcription factor in angiosperm［J］. Bot Stud， 2017， 58（1）： 6. DOI：10.1186/s40529-016-0159-1.
［42］ Zhu X， Qi L， Liu X， et al. The wheat ethylene response factor transcription factor pathogen-induced ERF1 mediates host responses to both the necrotrophic pathogen Rhizoctonia cerealis and freezing stresses［J］. Plant Physiol， 2014， 164（3）： 1499-1514. DOI：10.1104/pp.113.229575.
［43］ Zhao C， Zhang Z， Xie S， et al. Mutational evidence for the critical role of CBF transcription factors in cold acclimation in Arabidopsis［J］. Plant Physiol， 2016， 171（4）： 2744-2759. DOI：10.1104/pp.16.00533.
［44］ Liu J， Shi Y， Yang S. Insights into the regulation of C-repeat binding factors in plant cold signaling［J］. J Integr Plant Biol， 2018， 60（9）： 780-795. DOI：10.1111/jipb.12657.
［45］ Kasuga M， Liu Q， Miura S， et al. Improving plant drought， salt， and freezing tolerance by gene transfer of a single stress-inducible transcription factor［J］. Nat Biotechnol， 1999， 17（3）： 287-291. DOI：10.1038/7036.
［46］ Zhao C， Zhu J K. The broad roles of CBF genes： From development to abiotic stress［J］. Plant Signal Behav， 2016， 11（8）： e1215794. DOI：10.1080/15592324.2016.1215794.
［47］ Karaba A， Dixit S， Greco R， et al. Improvement of water use efficiency in rice by expression of HARDY， an Arabidopsis drought and salt tolerance gene［J］. PNAS， 2007， 104（39）： 15270-15275. DOI：10.1073/pnas.0707294104.
［48］ Cheng M C， Hsieh E J， Chen J H， et al. Arabidopsis RGLG2， functioning as a RING E3 ligase， interacts with AtERF53 and negatively regulates the plant drought stress response［J］. Plant Physiol， 2012， 158（1）： 363-375. DOI：10.1104/pp.111.189738.
［49］ Zhu D， Wu Z， Cao G， et al. TRANSLUCENT GREEN， an ERF family transcription factor， controls water balance in Arabidopsis by activating the expression of aquaporin genes［J］. Mol Plant， 2014， 7（4）： 601-615. DOI：10.1093/mp/sst152.
［50］ Xu Z Y， Kim D H， Hwang I. ABA homeostasis and signaling involving multiple subcellular compartments and multiple receptors［J］. Plant Cell Rep， 2013， 32（6）： 807-813. DOI：10.1007/s00299-013-1396-3.
［51］ Gilmour S J， Fowler S G， Thomashow M F. Arabidopsis transcriptional activators CBF1， CBF2， and CBF3 have matching functional activities［J］. Plant Mol Biol， 2004， 54（5）： 767-781. DOI：10.1023/B： PLAN.0000040902.06881.d4.
［52］ Lata C R， Prasad M. Role of DREBs in regulation of abiotic stress responses in plants［J］. J Exp Bot， 2011， 62（14）： 4731-4748. DOI：10.1093/jxb/err210.
［53］ Hsieh E J， Cheng M C， Lin T P. Functional characterization of an abiotic stress-inducible transcription factor AtERF53 in Arabidopsis thaliana［J］. Plant Mol Biol， 2013， 82（3）： 223-237. DOI：10.1007/s11103-013-0054-z.
［54］ Papdi C， Pérez-Salamó I， Joseph M P， et al. The low oxygen， oxidative and osmotic stress responses synergistically act through the ethylene response factor VII genes RAP2.12， RAP2.2 and RAP2.3［J］. Plant J， 2015， 82（5）： 772-784. DOI：10.1111/tpj.12848.
［55］ Yao Y， Chen X， Wu A M. ERF-VII members exhibit synergistic and separate roles in Arabidopsis［J］. Plant Signal Behav， 2017， 12（6）： e1329073. DOI：10.1080/15592324.2017.1329073.
［56］ Liu P， Sun F， Gao R， et al. RAP2.6L overexpression delays waterlogging induced premature senescence by increasing stomatal closure more than antioxidant enzyme activity［J］. Plant Mol Biol， 2012， 79（6）： 609-622. DOI：10.1007/s11103-012-9936-8.

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