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CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2020, Vol. 42 ›› Issue (6): 1078-.doi: 10.19802/j.issn.1007-9084.2020193

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Response patterns of malonyl-CoA:ACP malonyltransacylase (MCMT) gene family to different stresses in peanut

  


  1. 1. Bio-Tech Research Center, Shandong Academy of Agricultural Science / Shandong Provincial Key Laboratory of
    Genetic Improvement, Ecology and Physiology of Crops, Jinan 250100, China; 2. Shandong Peanut Research Institute, Qingdao 266100, China; 3. Shandong Academy of Agricultural Science, Jinan 250100, China; 4. Scientific Observation and Experiment Station of Crop Cultivation in East China, Ministry of Agriculture
    and Rural Affairs, Dongying 257000, China
  • Online:2020-12-28 Published:2020-11-19

Abstract:  Malonyl-CoA:ACP malonyltransacylase (MCMT) catalyzed the formation from malonyl-CoA to mal⁃
onyl monoacyl ACP. It is not only a basic component of the synthesis of biological fatty acids, but also an important
component of cell membrane, lipid storage and lipid signaling molecules. But so far there has been little study on
this gene family. Peanut (Arachis hypogaea L.) is a major oil and cash crop in China, it is very important to delve in⁃
to the mechanism of oil synthesis of peanut. In this paper, members of peanut MCMT gene family were systematical⁃
ly analyzed. In cultivated peanut genome, 5 AhMCMTs genes were distributed on 5 different chromosomes. All of
them contained more than one intron, with the number of 9-12. The length of the proteins varied from 277 to 472
amino acids without transmembrane structure, and were located in chloroplast. Three AhMCMTs were alternatively
spliced, among which arahy. FJ253B and arahy. R7F6LC each had 6 splicing variants, and arahy. T9C115 had 2
splicing variants. The main types of alternative splicing are intron retention, exon jumping, and variable splicing at
5’-UTR. Through the analysis of evolutionary tree, it was found that there were more MCMT genes in the genomes
of cultivated peanut, Brassica rapa, Camellia sinensis, Lupinus angustifolius, which may be caused by gene family
expansion. From peanut transcriptome data, results showed that their tissue-specific expression patterns were signif⁃
icantly different. Arahy.FJ253B had the highest expression level and was expressed in almost all tissues and organs,
which was speculated to be the main gene responsible to growth and development. Using peanut root and leaf tran⁃
scriptome data from different stress treatments of peanut young seedlings, these 5 AhMCMTs response varied under
different stresses, and had different expression patterns in roots and leaves. It indicated that 5 AhMCMTs had differ⁃
ent function division to stress.

Key words: Arachis hypogaea L., malonyl-CoA:ACP malonyltransacylase, alternative splicing, tissue-specific expression pattern, stress treatment


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