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CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2021, Vol. 43 ›› Issue (5): 933-.doi: 10.19802/j.issn.1007-9084.2020194

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Extraction and purification of flavonoids from peanut shell

  

  1. Shandong Peanut Reasearch Institute, Qingdao 266100, China
  • Online:2021-11-01 Published:2021-11-02

Abstract: In this study, the yield of total flavonoids from peanut shell was furthest increased by enzymatic pretreatment combined with ultrasonic assisted extraction, and the purity of active components was also improved by optimizing the purification process of macroporous resin. The optimal extraction process of flavonoids is as follows: the peanut shell powder was mixed with water, the dosage of hemicellulase combined with xylanase at 1:1 (m/m) was 0.25‰, the mixture was hydrolysed for 30 min at 50℃. After that, ethanol was added at the solid-liquid ratio of 1:20 (m/V) to a final concentration of 60%, then ultrasonic assisted extraction was performed at 1000 W and 55℃ for 60 min. The extraction ratio of total flavonoids under this extraction condition was about 2.5%. D101 macroporous resin was selected. The loading buffer was 60% ethanol solution with pH 5.0, and the eluent was 70% ethanol solution with pH 10.0. The flow rate of loading and elution was 0.75 BV/h and 1.5 BV/h. The purity of total flavone and luteolin from peanut shell were 10.54% and 5.85%, respectively, which were increased by 90% and 120%.

Key words: flavonoids from peanut shells, extraction, purification, luteolin

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