CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2020, Vol. 42 ›› Issue (6): 1090-.doi: 10.19802/j.issn.1007-9084.2020204

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Structure and expression analysis of mitochondrial malate dehydrogenase gene AhMMDH1 in peanut


  1. 1. College of Life Science, Shandong Normal University, Jinan 250014,China; 2. Biotechnology Research Center,Shandong Academy of Agricultural Sciences / Shandong Provincial Key Laboratory of Crop Genetic Improvement Ecology and Physiology, Jinan 250100,China
  • Online:2020-12-28 Published:2020-11-19

Abstract: Malate dehydrogenase (MDH) is a class of crucial enzyme in organisms, which involved in the regu⁃
lation of some important biological processes such as plant growth, pollen and fruit development. However, the func⁃
tion of malate dehydrogenase on after-ripening and germination of seeds has been hardly studied in plant. In this
study, the cDNA of AhMMDH1 gene in peanut was cloned, which has an ORF of 1038 bp in size, and encodes the
enzyme consisting of 345 amino acids. AhMMDH1 is a hydrophobic protein located on mitochondrion. Three-di⁃
mensional structure analysis found that AhMMDH1 existed as a heterodimer of malate dehydrogenase (M chain) and
glyoxylate precursor (G chain), and its M chain had a NAD+ binding site at 41-47. The qRT-PCR analysis found
that AhMMDH1 gene was highly expressed in flowers, followed by dry seeds, and expressed at trace level in stems
and leaves. The expression levels in seeds at different developmental stages from 10 d to 70 d (when freshly harvest⁃
ed) after pegging were significantly lower than that in dry seed. The expression of AhMMDH1 increased significantly
at imbibition for 16 hours, and reached the highest level when the radicle broke through the seed coat (at imbibition
for 36 hours), and then the expression decreased significantly. It is speculated that AhMMDH1 gene might play an
important role in after-ripening and germination. This study provided a foundation for further studying the biologi⁃
cal functions of AhMMDH1.

Key words:  , malate dehydrogenase;bioinformatics analysis;expression pattern analysis

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