CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2020, Vol. 42 ›› Issue (6): 1100-.doi: 10.19802/j.issn.1007-9084.2020213

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Genome-wide identification and expression analysis of auxin response factor gene family in Arachis hypogaea L.


  1. 1. Biotechnology Research Center, Shandong Academy of Agricultural Sciences/Shandong Provincial Key Laboratory
    of Crop Genetic Improvement, Ecology and Physiology, Jinan 250100, China; 2. College of Life Sciences, Shandong Normal University, Jinan 250014, China
  • Online:2020-12-28 Published:2020-11-19

Abstract:  Auxin response factor (ARF) is an essential transcription factor (TF) in auxin signal transduction
pathway. It plays key roles in many processes of plant growth and development, such as seeds gemination, organs
formation, fruits maturation and embryogenesis. To understand the characteristics of AhARF gene family in peanut,
62 AhARF genes were identified by bioinformatics methods. These genes were located on 18 chromosomes except
Chr1 and Chr11, and the amount of genes distributed on the corresponding chromosome of sub-genome A and B is
approximate. According to the phylogenic relationship of AtARF and AhARF genes, 62 AhARFs and partial AtARFs
were classified into the other four clades except for ClassIII (Arabidopsis special branch). 33 segmentally duplicated
gene pairs have been detected in AhARF gene family, and their ratios of Ka/Ks were all less than one, suggesting
that the purifying selection might participate in expansion of ARF genes during peanut evolution. In addition, their
physical and chemical properties, and the conserved domains of their proteins corresponding to the genes were pre⁃
dicted and analyzed by bioinformatics in this study. Furthermore, based on the transcriptome data of 22 different tis⁃
sues, the gene expression patterns of AhARF genes were displayed by heatmap. To explore the biological function of
AhARF10, AhARF20,AhARF23 and AhARF46, their spatio-temporal expression profiles were analyzed using realtime
PCR. The results of this study laid a foundation for further research on the function of ARF gene family in Ara⁃
chis hypogaea L.

Key words: Arachis hypogaea L.;auxin response factor gene family;phylogenic relationship, conserved domain of proteins, expression pattern analysis

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