Genetic model analysis of peanut pod traits in nested-crossing population

doi:10.19802/j.issn.1007-9084.2020247

Abstract

Abstract:

The genetic pattern of pod traits were analysed using the major gene plus polygene model in five combinations of F2 families of a nested crossing population in peanut to dissect the genetic variation in nested crossing population. Results revealed rich variations in the five pod traits in the nested combination. The ranges of pod length, pod width, and hundred-pod weight were（14.30-22.09）mm -（38.36-45.12）mm,（7.06-10.47）mm -（17.13-22.74）mm, and（62.41-94.38）g -（266.75-364.00）g, respectively. There was a strong positive correlation between pod length and pod width, pod surface area, pod surface perimeter and hundred-pod weight, but little correlation with ratio of pod length to pod width; pod width was positively correlated with pod surface area, pod surface perimeter and hundred-pod weight, but negatively correlated with pod length to width ratio. The genetic models of different pod traits in different combinations were different, and the best genetic models were two major gene of additive - dominant model and two major gene of additive - dominant - epistatic model. The heritability of major genes was 22.79% - 91.62%, and the genetic effects of the major genes in different families differed to each other, which implied the effects of multiple alleles or non-alleles as well as the genetic background on the pod traits. The results provide material and genetic basis for the further QTL mapping of pod traits and peanut breeding of specific pod shapes.

Key words: peanut (Arachis hypogaea l.), nested-crossing population, pod traits, major gene plus polygene model

CLC Number:

中图分类号：S565.2

ZHANG Mao-ning, ZHANG Xin-you, SUN Zi-qi, HUANG Bing-yan, LIU Hua, XU Jing, ZHANG Zhong-xin, QI Fei-yan, DONG Wen-zhao. Genetic model analysis of peanut pod traits in nested-crossing population[J]. CHINESE JOURNAL OF OIL CROP SCIENCES, 2021, 43(4): 573-.

References

［1］ Bertioli D J，Cannon S B，Froenicke L，et al. The genome sequences of Arachis duranensis and Arachis ipaensis，the diploid ancestors of cultivated peanut［J］.Nat Genet，2016，48（4）：438-446. DOI：10.1038/ng.3517.

［2］董文召，张新友，韩锁义，等. 中国与美国花生生产成本及收益比较分析［J］. 农业科技管理，2017（05）：60-64. DOI：10.16849/j.cnki.issn1001-8611.2017.05.017

［3］ Zhuang W，Chen H，Yang M，et al. The genome of cultivated peanut provides insight into legume karyotypes，polyploid evolution and crop domestication［J］. Nat Genet，2019，51（5）：865-876. DOI：10.1038/s41588-019-0402-2.

［4］李淞淋，曹永跃. 世界花生和花生油生产、贸易发展动态及结构特征［J］. 世界农业，2018（11）：113-119+253. DOI：10.13856/j.cn11-1097/s.2018.11.020

［5］ Chen Y，Ren X，Zheng Y，et al. Genetic mapping of yield traits using RIL population derived from Fuchuan Dahuasheng and ICG6375 of peanut（Arachis hypogaea L.）［J］. Molecular Breeding，2017，37（2）：17. DOI：10.1007/s11032-016-0587-3.

［6］李兰周，刘风珍，万勇善，等. 花生荚果和籽仁相关性状的主基因+多基因混合遗传模型分析［J］. 华北农学报，2013，28（5）：116-123. DOI：10.7668/hbnxb.

［7］刘佳琪，李英杰，杨会，等. 栽培种花生RIL群体荚果及籽仁性状遗传变异分析［J］. 山东农业科学，2017，49 （11）： 13-19. DOI： 10.14083/j. issn. 1001-4942.2017.11.002

［8］ Dwivedi N，Maji S，Waseem M，et al. The Mediator subunit OsMED15a is a transcriptional co-regulator of seed size/weight-modulating genes in rice［J］. Biochim Biophys Acta Gene Regul Mech， 2019， 1862（10）：194432. DOI：10.1016/j.bbagrm.2019.194432.

［9］ Su Q，Zhang X，Zhang W，et al. QTL detection for kernel size and weight in bread wheat（Triticum aestivum L.） using a high-density SNP and SSR-based linkage map［J］. Front Plant Sci，2018，9：1484. DOI：10.3389/fpls.2018.01484.

［10］ Xu Y，Li H N，Li G J，et al. Mapping quantitative trait loci for seed size traits in soybean （Glycine max L.Merr.）［J］. Theor Appl Genet，2011，122（3）：581-594. DOI：10.1007/s00122-010-1471-x.

［11］ Fonceka D，Tossim HA，Rivallan R，et al. Fostered and left behind alleles in peanut：interspecific QTL mapping reveals footprints of domestication and useful natural variation for breeding［J］. BMC Plant Biol，2012，12：26. doi：10.1186/1471-2229-12-26

［12］ Huang L，He H，Chen W，et al. Quantitative trait locus analysis of agronomic and quality-related traits in cultivated peanut （Arachis hypogaea L.）［J］. Theor Appl Genet， 2015，128（6）：1103-1115. doi：10.1007/s00122-015-2493-1

［13］李振动，李新平，黄莉，等. 栽培种花生荚果大小相关性状QTL 定位［J］. 作物学报，2015，41（09）：1313-1323. DOI：10.3724/SP.J.1006.2015.01313

［14］ Chen X，Lu Q，Liu H，et al. Sequencing of cultivated Peanut，Arachis hypogaea，yields insights into genome evolution and oil improvement［J］. Mol Plant，2019，12（7）：920-934. DOI：10.1016/j.molp.2019.03.005.

［15］ Luo H，Guo J，Ren X，et al. Chromosomes A07 and A05 associated with stable and major QTLs for pod weight and size in cultivated peanut（Arachis hypogaea L.）［J］. Theor Appl Genet. 2018；131（2）：267-282.DOI：10.1007/s00122-017-3000-7.

［16］周小静，雷永，夏友霖，等. 花生荚果大小和重量相关性状的QTL定位分析［J］. 中国油料作物学报，2019，41（06）： 869-877. DOI： 10.19802/j. issn. 1007-9084.2019125

［17］ Li H，Bradbury P，Ersoz E，Buckler ES，Wang J. Joint QTL linkage mapping for multiple-cross mating design sharing one common parent［J］. PLoS ONE. 2011，6（3）：e17573. DOI：10.1371/journal.pone.0017573.

［18］ Wang J，Yan C，Li Y，et al. GWAS discovery of candidate genes for yield-related traits in peanut and support from earlier QTL mapping studies［J］. Genes（Basel），2019，10（10）：803. DOI：10.3390/genes10100803.

［19］ Pandey M K，Upadhyaya H D，Rathore A，et al. Genomewide association studies for 50 agronomic traits in peanut using the 'reference set' comprising 300 genotypes from 48 countries of the semi-arid tropics of the world［J］. PLoS ONE，2014，9（8）：e105228. DOI：10.1371/journal.pone.0105228.

［20］ Cardon L R，Palmer L J. Population stratification and spurious allelic association［J］. Lancet， 2003， 361（9357）：598-604. DOI： 10.1016/S0140-6736（03）12520-2.

［21］王建康，盖钧镒. 利用杂种F2世代鉴定数量性状主基因-多基因混合遗传模型并估计其遗传效应［J］. 遗传学报， 1997， 24（5）： 432-440. DOI： 10.1007/BF02951625.

［22］盖钧镒，章元明，王建康. 植物数量性状遗传体系［M］. 北京：科学出版社，2003：96-101.

［23］姜慧芳，段乃雄. 花生种质资源描述规范和数据标准［M］. 北京：中国农业出版社，2006.

［24］房元瑾，孙子淇，苗利娟，等. 花生籽仁外观和营养品质特征及食用型花生育种利用分析［J］.植物遗传资源学报，2018，19（05）：875-886. DOI：10.13430/j.cnki.jpgr.20180110001

［25］王强. 花生加工品质学［M］. 北京：中国农业出版社，2013.54-55.

［26］ Song J M，Guan Z，Hu J，et al. Eight high-quality genomes reveal pan-genome architecture and ecotype differentiation of Brassica napus［J］. Nat Plants，2020，6（1）：34‐45. DOI：10.1038/s41477-019-0577-7.

［27］ Gangurde S S，Wang H，Yaduru S，et al. Nested-association mapping（NAM）-based genetic dissection uncovers candidate genes for seed and pod weights in peanut（Arachis hypogaea）［J］. Plant Biotechnol J，2020，18（6）：1457‐1471. DOI：10.1111/pbi.13311.

［28］殷冬梅，尚明照，崔党群. 花生主要农艺性状的遗传模型分析［J］.中国农学通报，2006（07）：261-265. DOI：10.3969/j.issn.1000-6850.2006.07.068

［29］刘华. 栽培花生产量和品质相关性状遗传分析与QTL定位研究［D］. 郑州：河南农业大学，2011. DOI：10.7666/d.y1999420

[1]	HUANG Bing-yan, HU Jing-zhi§, ZHANG Xin-you, MIAO Li-juan, SHI Lei, LV Deng-yu, CHAI Peng-pei, FENG Su-ping, LIU Hua, HAN Suo-yi, WANG Xiao, QI Fei-yan, SUN Zi-qi, QIN Li, DONG Wen-zhao. Genetic analysis of direct and maternal effects of fat content in peanut seed [J]. CHINESE JOURNAL OF OIL CROP SCIENCES, 2021, 43(4): 582-.
[2]	XU Man-lin, ZHANG Xia§, WU Ju-xiang, YU Jing, GUO Zhi-qing, LI Ying, XIE Hong-feng, TAN De-yun, ZHOU Ru-jun, CHI Yu-cheng. Evaluation of peanut accessions resistance against Phoma arachidicola and relationship between disease-resistance and yield loss [J]. CHINESE JOURNAL OF OIL CROP SCIENCES, 2021, 43(4): 731-.
[3]	XU Yang, ZHANG Guan-chu, DING Hong, ZHANG Zhi-meng, CI Dun-wei, GUO Feng, DAI Liang-xiang. Response of rhizosphere bacterial community structure associated with peanut (Arachis hypogaea L.) to high salinity and drought stress [J]. CHINESE JOURNAL OF OIL CROP SCIENCES, 2020, 42(6): 985-.
[4]	XU Ping-li, TANG Gui-ying, LI Guo-wei, FU Chun, LU Cheng-kai, JIANG Yan-sheng, SONG Xiao-feng, SHAN Lei, WAN Shu-bo. Application of MITE molecular marker of AhFAD2B gene in breeding high oleate peanut [J]. CHINESE JOURNAL OF OIL CROP SCIENCES, 2020, 42(6): 1069-.
[5]	CUI Wei-pei, TANG Gui-ying, XU Ping-li, LI Peng-xiang, ZHU Jie-qiong, SHAN Lei. Content changes of endogenous hormones in peanut seeds during germination [J]. CHINESE JOURNAL OF OIL CROP SCIENCES, 2020, 42(5): 869-.
[6]	XU Man-lin，WU Ju-xiang§，ZHANG Xia§，YAN Hong-hai，LYU Bin，YU Jian-lei，LIU Tong-jin，YU Jing，GUO Zhi-qing，CHI Yu-cheng，WAN Shu-bo . Identification of pathogenicity of Phoma arachidicola from different geographical location and the influence of environmental conditions on pathogenicity [J]. 中国油料作物学报, 2019, 41(2): 250-.
[7]	SHI Lei, HUANG Bing-yan, QI Fei-yan, MIAO Li-juan, LIU Hua, ZHANG Zong-xin, GAO Wei, DONG Wen-zhao, TANG Feng-shou, ZHANG Xin-you *. Cloning and functional analysis of AhFAE1 and its promoter from Arachis hypogaea L. [J]. 中国油料作物学报, 2019, 41(2): 176-.
[8]	SHI Lei, QI Fei-yan, MIAO Li-juan, HUANG Bing-yan, LIU Hua, ZHANG Zong-xin, GAO Wei, DONG Wen-zhao, TANG Feng-shou, ZHANG Xin-you . Cloning and functional analysis of a novel early embryo-specific expressed AhDGAT3 promoter from Arachis hypogaea L. [J]. 中国油料作物学报, 2018, 40(1): 25-.
[9]	LU Qing, LI Shao-xiong, CHEN Xiao-ping, ZHOU Gui-yuan, HONG Yan-bin, LI Hai-fen, LIANG Xuan-qiang* . Current situation, problems and suggestions of peanut breeding in southern China [J]. 中国油料作物学报, 2017, 39(4): 556-.
[10]	REN Xiao-ping§, ZHENG Yan-li§, HUANG Li, CHEN Yu-ning, ZHOU Xiao-jing, CHEN Wei-gang, LEI yong, YAN Li-ying,WAN Li-yun, LIAO Bo-shou, JIANGHui-fang* . Selection of core SSR markers and identification of fingerprint on peanut cultivars [J]. 中国油料作物学报, 2016, 38(5): 563-.
[11]	XIA You-lin, JING Yi-lin, MAO Jin-xiong, REN Xiao-ping, QI Yan, LIAO Jun-hua, ZENG Yan, REN Zheng-long, LIAO Bo-shou . Segregation analysis of resistance to late leaf spot in peanut [J]. 中国油料作物学报, 2015, 37(2): 134-.
[12]	CHENG Liang-qiang, HUANG Li, REN Xiao-ping, CHEN Yu-ning, ZHOU Xiao-jing, JIANG Hui-fang, LEI yong, HUANG Jia-quan, YAN Li-ying, LIAO Bo-shou*. SSR markers related to pod characteristics in new peanut cultivar Zhonghua 16 with high yield and oil content and its direct and indirect parents [J]. 中国油料作物学报, 2014, 36(6): 735-.