Cell division and signal transfer mechanism of Brassica napus mediated by reactive oxygen species

doi:10.19802/j.issn.1007-9084.2020275

Abstract

Abstract:

To understand the role of reactive oxygen (ROS) in cold resistance mechanism of Brassica napus,studies were carried on investigation of generation, distribution and signal transduction of ROS in B. napus cells. B.napus variety 16NTS309 was used as material for O2- sub-cellular localization by histochemical staining and organizational structure analysis. It was found that O2- signal was detected in meristem of root tip, meristem of shoot tip,leaf primordia, leaf margin and callus, which had strong ability of cell division under normal conditions. However,the plant underwent an oxidation explosion after cold stress. The sub-cellular distribution of O2- was found in root,petiole and leaves by morphoanatomical analysis. Tissue structure analysis showed that starch grains in petiole and mesophyll cells were homogeneous, and location of O2- in roots and calli was mainly in plasma membrane and cell wall. Diffusion of O2- was even found in surrounding cells. It suggested that O2- might be actively involved in regulating cell division. Vascular tissue system of B. napus might be capable to complete redox messenger synthesis, signal amplification and system transport. And cold response could be realized by ROS signaling between different tissues and over long distances organs in B. napus.

Key words: Brassica napus L., reactive oxygen species；cell division；vascular tissue；signal transduction；tissue section

CLC Number:

中图分类号：S565.4

QI Wei-liang, MA Li, WANG Ping, WANG Fei, SUN Bai-lin, WANG Wan-peng, WU Jun-yan, SUN Wan-cang. Cell division and signal transfer mechanism of Brassica napus mediated by reactive oxygen species[J]. CHINESE JOURNAL OF OIL CROP SCIENCES, 2021, 43(3): 470-.

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