Welcome to CHINESE JOURNAL OF OIL CROP SCIENCES,

CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2021, Vol. 43 ›› Issue (5): 762-.doi: 10.19802/j.issn.1007-9084.2020339

Previous Articles     Next Articles

Construction and phenotypic analysis of EMS-treated mutant library in spring Brassica napus L.

  

  1. Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China
  • Online:2021-11-01 Published:2021-11-01

Abstract: Creation of spring rapeseed mutant library is expected to be used in variety improvement and functional genomic research. Spring Brassica napus does not need a long vernalization process for flowering and has a short growth period, which makes it very promising for the creation of mutant banks in rapeseed. In this study, ethyl methanesulfonate (EMS) was used to mutate the seeds of spring rapeseed 862 to construct an EMS mutant library. By comparing the effects of 8 different concentrations of EMS (0-1.4%) on seeds germination rate, 0.8% EMS, which resulted in 37.2% germination rate, was used as the application concentration. Through field screening and phenotypic statistics, the morphological characteristics of 1823 individual plants in M2 generation were identified. The total mutation frequency was 3.91%. The mutant phenotypes mainly include leaf color, leaf shape, plant height, plant type, silique length, seed size, seed coat color, and seed oil content. The construction of this mutant library provided a variety of phenotypic germplasm resources for functional genomics research and molecular breeding in rapeseed.

Key words: spring Brassica napus L., EMS (ethyl methylsulfonate), mutation, trait screen

CLC Number: