Genetic diversity analysis ofdifferent cultivars of Camellia oleifera based on CDDP and ITS molecular markers

doi:10.19802/j.issn.1007-9084.2020344

Abstract

Abstract:

CDDP （conserved DNA-derived polymorphism） and ITS （internal transcribed spacer） molecular markers were used to evaluate the genetic diversity among 17 cultivars of Camellia oleifera in Jiangxi Province, so as to provide a theoretical basis for the identification and utilization of local Camellia oleifera varieties. Our results indicated that 170 polymorphic bands were amplified by 17 CDDP primers among 204 bands, and the ratio of polymorphic band was 83.33%. The 17 cultivars of Camellia oleifera could be identified by primers ERF2, ERF3 and WRKYR1 respectively. The SM （simple matching） similarity coefficients among varieties ranged from 0.5506 to 0.8544 with an average of 0.7438. And according to the UPGMA cluster analysis, 17 cultivars of Camellia oleifera could be divided into 5 categories，and tend to cluster according to economic traits. ITS sequence analysis showed that, the overall average genetic distance of 17 cultivars of Camellia oleifera was 0.0702. NJ phylogenetic tree analysis indicated that the genetic relationship among 17 Camellia oleifera varieties could be obviously reflected in phenotypic traits. This study shows that CDDP molecular markers combined with ITS sequence analysis technology could be effectively used for genetic diversity analysis of Camellia oleifera.

Key words: Camellia oleifera, ITS sequence analysis, CDDP molecular marker, genetic diversity

CLC Number:

S794.4

Zhi-hong WANG, Cheng-wen YONG, Gang CHEN, Yong-dian DING, Zhi-gang ZHAO, Ai-qing LYU, Cheng-yun HAN. Genetic diversity analysis ofdifferent cultivars of Camellia oleifera based on CDDP and ITS molecular markers[J]. CHINESE JOURNAL OF OIL CROP SCIENCES, 2022, 44(1): 94-102.

Figures/Tables 8

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编号 Number	品种名称 Variety	简称 Abbreviation	来源 Sources
1	长林4号 Changlin 4	CL4	宜春袁州区 Yuanzhou District， Yichun City
2	长林166号 Changlin 166	CL166	宜春袁州区Yuanzhou District， Yichun City
3	长林18号 Changlin 18	CL18	宜春袁州区Yuanzhou District， Yichun City
4	长林优株 Changlin youzhu	CLYZ	宜春袁州区Yuanzhou District， Yichun City
5	华兴1号 Huaxing 1	HX1	宜春袁州区Yuanzhou District， Yichun City
6	赣永5号 Hanyong 5	GY5	宜春袁州区Yuanzhou District， Yichun City
7	赣无夏讲 Ganwuxiajiang	GWXJ	宜春袁州区Yuanzhou District， Yichun City
8	赣石848号 Ganshi 848	GS848	宜春袁州区Yuanzhou District， Yichun City
9	湘林210号 Xianglin 210	XL210	宜春袁州区Yuanzhou District， Yichun City
10	赣兴48号 Ganxing 48	GX48	宜春袁州区Yuanzhou District， Yichun City
11	华硕9号 Huashuo 9	HS9	宜春袁州区Yuanzhou District， Yichun City
12	赣无二号 Ganwu 2	GW2	丰城白土 Baitu Town， Fengcheng City
13	长林40号 Changlin 40	CL40	宜春袁州区Yuanzhou District， Yichun City
14	长林53号 Changlin 53	CL53	宜春袁州区Yuanzhou District， Yichun City
15	长林27号Changlin 27	CL27	樟树观上Guanshang Town， Zhangshu City
16	长林3号 Changlin 3	CL3	宜春袁州区Yuanzhou District， Yichun City
17	赣无一号 Ganwu 1	GW1	丰城白土 Baitu Town， Fengcheng City

引物名称 Primer	序列5'-3' Sequences 5'-3'	退火温度/ ℃ Annealing temperature
WRKY-F1	TGGCGSAAGTACGGCCAG	47.0
WRKY-R1	GTGGTTGTGCTTGCC	48.3
WRKY-R3	GCASGTGTGCTCGCC	50.4
WRKY-R2B	TGSTGSATGCTCCCG	50.4
WRKY-R3B	CCGCTCGTGTGSACG	48.9
MYB1	GGCAAGGGCTGCCGC	52.1
MYB2	GGCAAGGGCTGCCGG	52.1
ERF2	GCSGAGATCCGSGACCC	53.2
ERF3	TGGCTSGGCACSTTCGA	49.8
KNOX-1	AAGGGSAAGCTSCCSAAG	49.8
KNOX-2	CACTGGTGGGAGCTSCAC	50.4
KNOX-3	AAGCGSCACTGGAAGCC	50.4
MADS-1	ATGGGCCGSGGCAAGGTGC	53.2
MADS-2	ATGGGCCGSGGCAAGGTGG	53.2
MADS-4	CTSTGCGACCGSGAGGTG	52.1
ABP1-1	ACSCCSATCCACCGC	50.4
ABP1-3	CACGAGGACCTSCAGG	50.4

引物名称 Primer	扩增条带数 Number of amplified bands	多态性条带数 Number of polymorphic bands	多态性比率/% Proportion of polymorphic bands /%	特异性条带数 Number of specific bands	特异性比率/% Proportion of specific bands /%
ABP1-1	16	15	93.75	3	18.75
ABP1-3	18	17	94.44	1	5.56
ERF2	12	12	100.00	1	8.33
ERF3	16	14	87.50	2	12.50
KNOX-1	16	12	75.00	2	12.50
KNOX-2	15	15	100.00	0	0.00
KNOX-3	9	5	55.56	1	11.11
MADS-1	10	10	100.00	0	0.00
MADS-2	12	12	100.00	2	16.67
MADS-4	11	9	81.82	2	18.18
MYB1	7	4	57.14	2	28.57
MYB2	6	4	66.67	1	16.67
WRKYF1	5	3	60.00	1	20.00
WRKYR1	14	12	85.71	0	0.00
WRKYR2B	14	8	57.14	1	7.14
WRKYR3	11	7	63.64	1	9.09
WRKYR3B	12	11	91.67	1	8.33
总计 Total	204	170		21
平均值 Mean	12.00	10.00	83.33	1.24	10.29

简称 Abbreviation	T（U）	C	A	G	GC%	序列长度 Sequence length
CL3	15.9	33.3	17.9	32.9	66.2	447.0
CL4	15.3	33.6	18.0	33.1	66.7	444.0
CL18	16.3	33.3	17.9	32.6	65.8	448.0
CL27	15.1	33.2	18.5	33.2	66.4	449.0
CL40	23.3	27.8	18.8	30.0	57.8	446.0
CL53	15.7	33.2	17.9	33.2	66.4	452.0
CL166	15.8	33.3	18.1	32.8	66.1	448.0
CLYZ	15.6	33.1	18.7	32.7	65.8	450.0
GS848	18.4	32.1	16.6	33.0	65.0	446.0
GW1	15.7	33.3	17.9	33.1	66.4	447.0
GW2	15.9	33.3	17.7	33.1	66.4	447.0
GWXJ	16.0	33.1	18.5	32.4	65.5	444.0
GX48	15.6	33.2	18.3	33.0	66.1	449.0
GY5	15.3	33.5	18.0	33.3	66.7	445.0
HS9	15.7	33.0	18.4	32.8	65.9	451.0
HX1	15.4	33.2	18.3	33.2	66.4	449.0
XL210	15.6	32.8	19.0	32.6	65.4	448.0

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