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CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2021, Vol. 43 ›› Issue (6): 1016-1024.doi: 10.19802/j.issn.1007-9084.2020353

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Identification of saline-alkali tolerant germplasm resources of soybean during the whole growth stage

Yi-qiang HAN1,3(), Ya-mei GAO1, Yan-li DU2, Yu-xian ZHANG2,3, Ji-dao DU2,3(), Wen-hui ZHANG2, Shao-yu PAN4   

  1. 1.College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China
    2.College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163319, China
    3.National Coarse Cereals Engineering Research Center, Daqing 163319, China
    4.Daqing Tannong Edible Mushrooms Technology Co. , Ltd, Daqing 163217, China
  • Received:2020-12-11 Online:2021-12-22 Published:2021-12-23
  • Contact: Ji-dao DU E-mail:hyq420@163.com;djdbynd0@163.com

Abstract:

Saline-alkali stress always exists in all stages of crop, and it is of great significance to screen saline-alkali tolerant soybean resources in the whole growth period. The salt species, salt content and pH value of saline-alkali soil in Daqing area were firstly measured, then the salt species and concentration of salt for screening were determined. In culture dish, germination bag and pot screening experiment, the saline-alkali tolerance of soybean was evaluated by the germination rate, the amount of matter at seedling stage and the dry matter weight at maturity stage. The results showed that the soil in Daqing area was sulfate soda saline-alkali soil, and the saline alkali solution is the mixed saline alkali solution of NaCl, Na2CO3, NaHCO3 and Na2SO4 (molar ratio is 1:1:9:9), and the total salt concentration calculated by Na ion content is 80 mmol · L-1, and the pH value of the solution is 8.9. Therefore, the salt content and pH value of soil in pot experiment were 3.3 ‰ and 8.9, respectively. Among the 887 soybean cultivars identified, the seed coat colors were black, brown, red, green, yellow and double color, and the correlation analysis showed that there was a significant positive correlation between salt tolerance and seed coat color of soybean. 296 tolerance soybean cultivars were selected from 887 soybean resources in germination stage, and 123 cultivars among 296 cultivars were identified as high resistance in germination and seedling stage, furthermore 7 resistant soybeans, including 5 from South China, 2 from Huanghuai, and 62 tolerant soybeans were obtained in the whole growth stage, and the proportion of pot screening was 56.10%. This study established a stepwise screening method from germination stage, seedling stage to the whole growth stage, and 69 salt and alkali resistant resources were selected from 887 soybean germplasm resources, which provided material basis for salt tolerance breeding and utilization of salt tolerant gene resources.

Key words: soybean resources, whole growth stage, saline-alkali tolerance, screening method

CLC Number: