QTL mapping for 100-pod and 100-seed weights in cultivated peanut

doi:10.19802/j.issn.1007-9084.2021015

Abstract

Abstract:

A peanut recombinant inbred population with Huayu 28 and P76 as parents was used to perform genetic mapping for 100-pod and 100-seed weight. Phenotypic data of 100-pod and 100-seed weight were collected in 3 environments. QTL mapping analysis was performed with individual environmental analysis and multi-environmental joint analysis. Multi-environmental joint analysis demonstrated 5 QTLs for 100-pod weight and 10 QTLs for 100-seed weight. Individual environmental analysis yielded 3 QTLs for 100-pod weight (qHPW05.1, qHPW07.1, qHPW19.1) on 3 linkage groups, and 4 QTLs for 100-seed weight (qHSW05.1, qHSW07.1, qHSW19.1, qHSW20.1) on 4 linkage groups. Among these, qHPW07.1, qHPW19.1, qHSW 07.1 and qHSW 19.1 could be detected in two or three environments with phenotypic variation explained (PVE) ranging from 4.610%-8.840%, 9.985%-11.224%, 7.155%-10.464% and 7.239%-13.845%. qHPW07.1 could be detected in 3 environments with phenotypic variation explained (PVE) of 4.610%-8.840%. Comparative analysis of all QTLs revealed two QTL clusters on LG07 (Cluster I) and LG19 (ClusterⅡ). These results provided a theoretical basis for both improvement of peanut yield and cloning of associated genes.

Key words: peanut, 100 pod weight, 100 seed weight, QTL

CLC Number:

S565.2

Feng-gao CUI, Xiao-hui HU, Hua-rong MIAO, Sheng-zhong ZHANG, Juan WANG, Song WANG, Gang HOU, Jie SUI, Jian-cheng ZHANG, Jing CHEN. QTL mapping for 100-pod and 100-seed weights in cultivated peanut[J]. CHINESE JOURNAL OF OIL CROP SCIENCES, 2021, 43(6): 1025-1030.

Figures/Tables 4

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性状Trait	环境 Enviroments	亲本Parents		重组自交系群体RILs population
性状Trait	环境 Enviroments	花育28号 Huayu28	P76	极小值Min	极大值Max	均值±标准误 Mean±SE	偏度 Skew	峰度 Kurt
百果质量（HPW） 100-pod weight / g	保定2011 2011HB	182.00	178.00	98.00	262.00	168.15±3.66	-0.18	1.32
	莱西2018 2018LX	192.00	168.00	120.00	304.00	194.83±2.98	0.46	0.37
	东营2018 2018DY	178.00	176.00	88.00	308.00	175.23±3.03	0.68	0.98
百仁质量（HSW） 100-seed weight / g	保定2011 2011HB	71.00	68.00	39.00	97.00	65.76±1.53	0.10	0.08
	莱西2018 2018LX	78.00	60.00	44.00	128.00	77.29±1.24	0.55	1.03
	东营2018 2018DY	70.00	65.00	35.000	120.00	73.88±1.14	0.42	0.75

性状 Trait	基因座 QTL	环境 Enviroments	位置 Position/cM	标记区间 Marker interval	LOD	加性效应ADD	表型贡献率 PVE/%
百果质量 100-pod weight	qHPW05.1	2018DY	62.535-72.485	PM297-Marker2773545	4.929	-13.148	11.932
	qHPW07.1	2018LX	1.454-3.463	Marker705764- Marker738964	2.587	8.083	4.610
		2011HB	1.454-3.463	Marker705764- Marker738964	2.555	9.275	6.079
		2018DY	1.454-3.463	Marker705764- Marker738964	4.196	11.317	8.840
	qHPW19.1	2018DY	59.563-61.591	Marker1737247-Marker1724074	2.606	-12.027	9.985
	qHPW19.1	2018LX	59.563-61.591	Marker1737247-Marker1724074	3.547	-12.613	11.224
百仁质量 100-seed weight	qHSW05.1	2018LX	3.582-3.942	Marker2890101-Marker2827232	2.897	-4.670	8.838
	qHSW07.1	2011HB	0-1.454	FI287928- Marker705764	3.604	3.582	10.464
	qHSW07.1	2018DY	0-1.454	FI287928- Marker705764	4.153	3.837	7.155
	qHSW19.1	2011HB	59.563-61.591	Marker1737247-Marker1724074	3.304	-2.979	7.239
		2018DY	59.563-61.591	Marker1737247-Marker1724074	5.207	-5.338	13.845
		2018LX	59.563-61.591	Marker1737247-Marker1724074	2.749	-4.444	8.004
	qHSW20.1	2018DY	40.368-45.8	Marker1737247-Marker3398822	2.567	3.235	5.087

性状 Trait	基因座 QTL	染色体 Chr	标记区间 Marker interval	LOD值 LOD	加性LOD值 LOD（A）	环境LOD值 LOD（AbyE）	表型贡献率 PVE /%	加性表型贡献率 PVE（A） /%	环境表型贡献率PVE （AbyE） /%	加性效应 Add	置信区间 Confidence interval /cM
百果质量 100-pod weight （HPW）	qMHPW-05.1	5	PM297-Marker2773545	7.294	3.969	3.325	7.691	4.705	2.986	-6.477	62.5-73.5
	qMHPW07.1	7	Marker738964-Marker641323	7.699	7.405	0.294	9.089	9.036	0.053	9.147	3.5-7.5
	qMHPW08.1	8	Marker3523489-Marker3487470	5.140	4.191	0.948	6.046	5.244	0.802	7.354	119.5-122.5
	qMHPW13.1	13	Marker4198185-Marker4329090	4.192	1.446	2.746	4.976	1.793	3.183	-4.066	66.5-69.5
	qMHPW19.1	19	Marker1737247-Marker1724074	6.591	5.634	0.957	8.458	7.040	1.418	-7.908	58.5-61
百仁质量 100-seed weight （HSW）	qMHSW05.1	5	Marker2825272-Marker2890101	4.610	2.890	1.720	5.526	2.855	2.671	-1.787	2.5-3.5
	qMHSW05.2	5	Marker2806182-Marker2791284	4.100	1.925	2.175	4.154	1.945	2.209	-1.500	65.5-77.5
	qMHSW07.1	7	FI287928-Marker705764	5.306	3.545	1.761	4.463	3.674	0.789	2.077	0-1.5
	qMHSW07.2	7	Marker738964-Marker641323	6.064	5.554	0.510	6.475	5.349	1.126	2.507	4.5-9.5
	qMHSW08.1	8	Marker3488622-Marker3523489	4.039	3.923	0.116	4.106	3.671	0.435	2.199	115.5-124.5
	qMHSW08.2	8	Marker3513718-Marker3516032	3.534	2.656	0.878	2.700	2.435	0.265	1.759	126.5-130
	qMHSW14.1	14	Marker4482927-Marker4479275	2.897	0.535	2.362	3.602	0.574	3.028	-0.804	13.5-14.5
	qMHSW14.2	14	Marker4579704-Marker4411597	3.948	1.958	1.990	3.722	1.944	1.778	1.482	154.5-158
	qMHSW15.1	15	TC23F09-Marker3782027	2.943	1.051	1.892	2.086	1.092	0.994	1.112	0-1.5
	qMHSW19.1	19	Marker1737247-Marker1724074	13.977	11.606	2.371	13.906	12.248	1.658	-3.723	59.5-61

染色体Chr	位置 Position	多环境联合分析 Multi-environmental joint analysis			单环境分析 Individual environment analysis
染色体Chr	位置 Position	基因座 QTL	标记 Marker	加性效应 ADD	基因座 QTL	环境 Enviroments	标记 Marker	加性效应 ADD
7	0-1.5	qMHSW07.1	FI287928- Marker705764	2.077	qHSW07.1	2011HB	FI287928-Marker705764	3.582
	0-1.5	qMHSW07.1	FI287928- Marker705764	2.077	qHSW07.1	2018DY	FI287928-Marker705764	3.837
	1.454-3.463				qHPW07.1	2018LX	Marker705764- Marker738964	8.083
						2011HB	Marker705764- Marker738964	9.275
						2018DY	Marker705764- Marker738964	11.317
	3.5-7.5	qMHPW07.1	Marker705764- Marker641323	9.147
	4.5-9.5	qMHSW07.2	Marker705764- Marker641323	2.507
19	58.5-61	qMHPW19.1	Marker1737247-Marker1724074	-7.908
	59.5-61	qMHSW19.1	Marker1737247-Marker1724074	-3.723
	59.563-61.591				qHPW19.1	2018DY	Marker1737247-Marker1724074	-12.027
					qHPW19.1	2018LX	Marker1737247-Marker1724074	-12.613
					qHSW19.1	2011HB	Marker1737247-Marker1724074	-2.979
						2018DY	Marker1737247-Marker1724074	-5.338
						2018LX	Marker1737247-Marker1724074	-4.444

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