
Transcriptome sequencing analysis of dwarfing traits in Brassica napus line DW871
Jing LUO, Chao LI, Rui-mao ZHANG, De-gang ZHAO, Zhi-hong GAO, Fang WANG, Yuan-yu YANG, Zhuan-zhuan WANG, Min WANG
CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2022, Vol. 44 ›› Issue (1) : 14-24.
Transcriptome sequencing analysis of dwarfing traits in Brassica napus line DW871
DW871 is a Brassica napus dwarf line with unusual and excellent plant type. To study the dwarf-related genes and their expression patterns of DW871, we took B. napus HW871 as control and selected stem tissues at the bolting stage for transcriptome gene expression analysis. Results showed that there were 8665 significant differentially expressed genes between DW871 and HW881, of which 2582 were up-regulated and 6083 down-regulated. The enrichment analysis of GO could be annotated to 22 subcategories in 3 categories, the enrichment analysis of KEGG was annotated to 295 pathways in 31 subcategories in 5 categories. We screened genes closely related to dwarfing traits from significantly different genes, 43 in pectin degradation, 69 in auxin signal transduction, and annotated 22 in the monolignol biosynthesis. It was found that the dwarfing character of DW871 was related to cell elongation, abnormal cell wall formation and cell division, and obstruction of auxin signal transduction. In particular, in monolignol biosynthesis, it is possible to regulate a series of physiological and biochemical processes related to lignin by regulating the expression of enzymes such as PAL and CCR. The differential genes related to dwarfing traits were screened in this research. It could be hypothesized that the dwarfing mechanism of DW871 involving abnormal auxin response and lignin synthesis, which lead to abnormal cell elongation, growth and cell wall changes, and ultimately lead to the dwarfing of DW871.
Brassica napus L. / transcription / dwarf trait / DW871 / plant type {{custom_keyword}} /
Fig. 1 Gene coverage graph图1 基因覆盖率 |
Fig. 4 KEGG enrichment图4 KEGG富集 |
Table 1 Genes related to monolignol synthesis and their expression patterns in B. napus DW871表1 甘蓝型油菜DW871中与木质素单体合成相关基因及其表达模式 |
编码的酶 Encoding enzyme | 基因名称 Gene name | 酶代码 EC number | 基因数量与表达方式 Gene expression |
---|---|---|---|
苯丙氨酸解氨酶 Phenylalanine ammonia-lyase | PAL | 4.3.1.24 | 3;1 up,2 down |
反肉桂酸4-单加氢酶 Trans-cinnamate 4-monooxygenase | CYP73A | 1.14.14.91 | 3;0 up,3 down |
4-香豆酸酯-CoA连接酶 4-coumarate--CoA ligase | 4CL | 6.1.2.12 | 1;O up,1 down |
莽草酸/奎宁酸羟基肉桂酰转移酶 Shikimate O-hydroxycinnamoyltransferase | HCT | 2.3.1.133 | 3;0 up,3 down |
肉桂酰辅酶A还原酶 Cinnamoyl-CoA reductase | CCR | 1.2.1.44 | 2;0 up,2 down |
肉桂醇脱氢酶 Cinnamyl-alcohol dehydrogenase | CAD | 1.1.1.195 | 2;0 up,2 down |
咖啡酸3-O-甲基转移酶 Caffeic acid 3-O-methyltransferase | COMT | 2.1.1.68 | 4;1 up,3 down |
咖啡酰-CoA O-甲基转移酶 Caffeoyl-CoA O-methyltransferase | CCoAOMT | 2.1.1.104 | 4;1 up,3 down |
Table 2 Genes related to pectin degradation and their expression patterns in B. napus DW871表2 甘蓝型油菜DW871中与果胶降解相关基因及其表达模式 |
编码的酶 Encoding enzyme | 基因名称 Gene name | 酶代码 EC number | 基因数量与表达方式 Gene expression |
---|---|---|---|
果胶酯酶 Pectinesterase | PME | 3.1.1.11 | 32;12 up,12 down |
多聚半乳糖醛酸酶 Polygalacturonase | PG | 3.2.1.15 | 7;0 up,7 down |
半乳聚糖1,4-α半乳糖醛酸酶 Galacturan 1,4-alpha-galacturonidase | PGA | 3.2.1.67 | 4;3 up,1 down |
Table 3 Genes related to auxin signaling and their expression patterns in B. napus DW871表3 甘蓝型油菜DW871中与生长素信号转导相关基因及其表达模式 |
编码的蛋白或小RNA Encoding protein and miRNA | 基因名称 Gene name | 酶代码 EC number | 基因数量与表达方式 Gene expression |
---|---|---|---|
生长素输入载体 Auxin influx carrier | AUX1 | - | 5;1 up,4 down |
生长素反应蛋白 Auxin-responsive protein IAA | AUX/IAA | - | 10;2 up,8 down |
生长素应答因子 Auxin response factor | ARF | - | 1;1 up,0 down |
生长素酰胺合成酶 Gretchen hagen 3 | GH3 | - | 13;1 up,12 down |
生长素上调小RNA Small auxin-up RNA | SAUR | - | 40;4 up,36 down |
Fig. 5 DW871 significant difference gene qRT-PCR verification图5 DW871显著差异基因qRT-PCR验证 |
1 |
刘成, 冯中朝, 肖唐华, 等. 我国油菜产业现状、潜力及对策[J]. 中国油料作物学报, 2019, 41(4): 485-489.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
2 |
王汉中. 我国油菜产业发展的历史回顾与展望[J]. 中国油料作物学报, 2010, 32(2): 300-302.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
3 |
沈金雄. 甘蓝型油菜杂种优势及其遗传分析[D]. 武汉: 华中农业大学, 2003.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
4 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
5 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
6 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
7 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
8 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
9 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
10 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
11 |
程曦, 郝怀庆, 彭励. 植物细胞壁中纤维素合成的研究进展[J]. 热带亚热带植物学报, 2011, 19(3): 283-290. DOI:10.3969/j.issn.1005-3395.2011.03.015 .
{{custom_citation.content}}
{{custom_citation.annotation}}
|
12 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
13 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
14 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
15 |
安文燕, 孙君灵, 龚文芳, 等. 陆地棉矮化突变体Ari1327茎尖的转录组分析[J]. 植物遗传资源学报, 2014, 15(5): 1046-1052. DOI:10.13430/j.cnki.jpgr.2014.05.019 .
{{custom_citation.content}}
{{custom_citation.annotation}}
|
16 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
17 |
牛显飞, 蒋学飞, 解艳芳, 等. 甘蓝型油菜矮化突变体的茎秆发育转录组分析[J]. 四川大学学报(自然科学版), 2018, 55(1): 201-206. DOI:10.3969/j.issn.0490-6756.2018.01.033 .
{{custom_citation.content}}
{{custom_citation.annotation}}
|
18 |
张玲, 郭爽, 汪玲, 等. 水稻矮化并花发育异常突变体dwarf and deformed flower2 (ddf2)的基因定位与候选基因分析[J]. 中国农业科学, 2015, 48(10): 1873-1881.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
19 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
20 |
张瑞茂, 李超, 陈大伦, 等. 甘蓝型油菜矮杆直立株型材料DW871的选育[J]. 种子, 2019, 38(2): 116-120, 123. DOI:10.16590/j.cnki.1001-4705.2019.02.116 .
{{custom_citation.content}}
{{custom_citation.annotation}}
|
21 |
张瑞茂, 李超, 陈大伦, 等. 甘蓝型油菜特异矮秆直立株型新品系DW871株高的遗传分析[J]. 中国油料作物学报, 2019, 41(1): 1-9.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
22 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
23 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
24 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
25 |
魏灵珠, 程建徽, 李琳, 等. 赤霉素生物合成与信号传递对植物株高的调控[J]. 生物工程学报, 2012, 28(2): 144-153. DOI:10.13345/j.cjb.2012.02.002 .
{{custom_citation.content}}
{{custom_citation.annotation}}
|
26 |
李波, 梁颖, 柴友荣. 植物肉桂酰辅酶A还原酶(CCR)基因的研究进展[J]. 分子植物育种, 2006, 4(z1): 55-65.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
27 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
28 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
29 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
30 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
31 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
32 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
33 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
34 |
徐洋. 分子调控咖啡酸-O-甲基转移酶(COMT)对木质素合成及植物生长发育的影响[D]. 北京: 中国科学院研究生院(植物研究所), 2003.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
35 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
36 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
37 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
38 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
39 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
40 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
41 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
42 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
43 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
44 |
张赛娜. OsARF19调控水稻叶夹角的分子机制[D]. 杭州: 浙江大学, 2014.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
45 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
46 |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
47 |
李盼, 李超, 张瑞茂, 等. 甘蓝型油菜矮秆新品系DW871在两环境下收获指数及相关性状分析[J]. 中国油料作物学报, 2020, 42(2): 240-248. DOI:10.19802/j.issn.1007-9084.2019223 .
{{custom_citation.content}}
{{custom_citation.annotation}}
|
{{custom_ref.label}} |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
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