Genome-wide identification and expression analysis of AOC gene family in Brassica napus L.

doi:10.19802/j.issn.1007-9084.2021070

Abstract

Abstract:

Allene oxide cyclase（AOC）is an important synthase in jasmonic acid（JA）signal pathway, whichplays an important role in many growth and development processes, such as photosynthesis, material metabolism and stress response. In this study, based on 4 AOC genes of Arabidopsis thaliana, 12, 4 and 6 AOC genes were identified in Brassica napus, Brassica rapa and Brassica oleracea. Then the molecular characteristics, protein conserved domains, phylogenetic tree, gene structure and chromosome distribution, tissue expression patterns under different stresses and hormone treatments were systematically compared and analyzed. The results showed that AOC gene wa highly conserved in the above Brassicaceae species, and AOC1/2/3 gene existed in the form of tandem gene cluster;12 BnaAOC genes had different tissue expression patterns and responded to various stresses and hormone treatments; BnaAOC3（BnaA09g19550D and BnaC09g52550D）genes were induced by methyl jasmonate（MeJA）, salicylic acid（SA）, salt stress and PEG-6000 treatment, and strongly induced by Sclerotinia sclerotiorum. This study
might lay a foundation and provide guidance for further understanding the biological functions of AOC family genesin response to abiotic stress and S. sclerotiorum in Brassica napus.

Key words: Brassica napus L., AOC gene, jasmonic acid, phylogenic relationship, Sclerotium sclerotiorum, abioitc stress

CLC Number:

中图分类号：S565.4

KANG Yu, LIU Wei, XIE Pan, GUAN Mei, QIAN Lun-wen, GUAN Chun-yun, HUA Wei, HE Xin. Genome-wide identification and expression analysis of AOC gene family in Brassica napus L.[J]. CHINESE JOURNAL OF OIL CROP SCIENCES, 2021, 43(3): 405-.

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