Cloning and activity of BnLEC1.A07 gene promoter in rapeseed (Brassica napus L.) lines with different oil content

doi:10.19802/j.issn.1007-9084.2021092

Abstract

Abstract:

In order to further study the gene BnLEC1 on oil biosynthesis, high-oil variety 61616 and low-oil variety 51070 were used as materials to carry out transcriptome and quantitative PCR analysis. The results showed that the expression level of BnLEC1. A07 in 61616 was significantly higher than that in 51070. Cloning and sequence comparison of the promoter PLEC161616 (1829 bp) and PLEC151070 (1824 bp) were performed respectively.The results showed that a large number of SNPs and InDels between the two promoters, and single-base SNPs were abundant. Cis-acting elements analysis showed that PLEC161616 had one CAAT-box and two jasmonic acid response elements more than those of PLEC151070, and one cell cycle control element and two abiotic stress response elements were missing. The promoter fusion vectors with GUS and LUC were constructed respective. GUS staining, LUC fluorescence imaging in vivo after transient transformation of tobacco leaves and luciferase activity assay showed that the activity of PLEC161616 was higher than that of PLEC151070. The comprehensive results showed that the expression level of BnLEC1.A07 gene in different lines was positively correlated with oil content. PLEC161616 had stronger priming activity than PLEC151070. This study provides a theoretical basis for further exploring the regulation mechanism of the lipid synthesis gene BnLEC1.A07 in different rapeseed lines.

Key words: , BnLEC1；promoter；activity；GUS；LUC

CLC Number:

WANG Yan-jia, FAN Shi-hang§, LIU Jing, ZHENG Ming, HUA Wei. Cloning and activity of BnLEC1.A07 gene promoter in rapeseed (Brassica napus L.) lines with different oil content[J]. CHINESE JOURNAL OF OIL CROP SCIENCES, 2021, 43(3): 383-.

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