QTL mapping and candidate genes identification for flowering time of soybean

doi:10.19802/j.issn.1007-9084.2021101

Abstract

Abstract:

Soybean is sensitive to photoperiod, and the cultivation area of each soybean variety is restricted to a very narrow latitudinal range. The adaptability of soybean between different latitudes is closely related to flowering time. In order to excavate the genes controlling flowering time of soybean, we performed QTL mapping for flowering time by using recombinant inbred line (RIL) population derived from Huapidou × Qihuang 26 cross and high-density linkage map constructed based on specific length amplified fragment sequencing (SLAF-seq). In total, 11 QTLs were identified on 7 chromosomes. Of them, 4 QTLs (qFT8, qFT20-2, qFT14 and qFT16) were newly identified in this study. Furthermore, 6 QTLs (qFT6-1, qFT8, qFT11-1, qFT19, qFT20-1 and qFT20-2) exist stably in 2 environments. By performing the sequences and bioinformatics analysis of genes between 6 stable QTL intervals, 4 candidate genes related to flowering time regulation had been identified. The results of this research were expected to provide a foundation for understanding the molecular mechanism of soybean adaptability and cultivating the varieties with wide adaptability.

Key words: soybean, flowering time, QTL, candidate genes identification

CLC Number:

S565.1

Wei LIU, Yu-bin WANG, Wei LI, Li-feng ZHANG, Cai-jie WANG, Ran XU, Hai-ying DAI, Yan-wei ZHANG. QTL mapping and candidate genes identification for flowering time of soybean[J]. CHINESE JOURNAL OF OIL CROP SCIENCES, 2022, 44(3): 548-554.

Figures/Tables 7

Table 1

Fig. 1

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Table 2

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Fig. 3

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QTL	环境 Environments	染色体 Chromosome	标记间距离 Interval /cM	LOD	PVE /%	Add	已报道的QTL Reported QTL
qFT6-1	2013	6	73.65~73.65	10.92	8.25	1.41	First flower26-2^［12］
qFT6-1	2014	6	73.65~73.65	13.55	7.13	1.24	First flower26-2^［12］
qFT8	2013	8	0.00~0.69	3.83	1.75	0.65
qFT8	2014	8	0.00~0.69	7.37	2.58	0.75
qFT11-1	2013	11	66.96~67.44	8.75	7.17	-1.34	First flower8-4^［13］
qFT11-1	2014	11	66.96~67.44	12.95	6.74	-1.22	First flower8-4^［13］
qFT19	2013	19	35.14~36.50	6.33	4.40	1.03	First flower15-2^［14］
qFT19	2014	19	35.14~36.50	9.69	4.69	1.00	First flower15-2^［14］
qFT20-1	2013	20	113.99~114.33	4.96	4.29	1.04	First flower16-3^［15］
qFT20-1	2014	20	113.99~114.33	10.64	6.85	1.23	First flower16-3^［15］
qFT20-2	2013	20	114.33~114.66	5.18	4.39	1.05
qFT20-2	2014	20	114.33~114.66	10.65	6.79	1.23
qFT6-2	2013	6	68.71~73.65	11.01	8.04	1.39	First flowe26-2^［12］
qFT11-2	2013	11	65.87~66.96	8.84	7.39	-1.36	First flower8-4^［13］
qFT11-3	2014	11	67.44~68.26	12.63	6.67	-1.21	First flower8-4^［13］
qFT14	2014	14	48.62~48.75	5.58	4.38	-0.98
qFT16	2014	16	62.43~65.28	5.65	3.08	-0.81

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