CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2022, Vol. 44 ›› Issue (3): 539-547.doi: 10.19802/j.issn.1007-9084.2021117

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Distinguishing and evaluating high nitrogen-use-efficient soybean germplasm at seedling stage

Yue-li YUAN1,2(), Yuan-yuan YI3, Yong ZHAN4, Li-miao CHEN1, Song-li YUAN1, Yi HUANG1, Zhi-yuan XIAO1, Chan-juan ZHANG1(), Xin-an ZHOU1()   

  1. 1.Oil Crops Research Institute of Chinese Academy of Agricultural Sciences,Key Laboratory of Biology and Genetics Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Wuhan 430062, China
    2.Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China
    3.Institute of Crop Resistance and Chemical Ecology, Agricultural College, Fujian Agriculture and Forestry University, Fuzhou 350000, China
    4.Crop Research Institute, Xinjiang Academy of Agricultural Reclamation Sciences / Shihezi Comprehensive Test Station of National Soybean Industrial Technology System / Key Laboratory of Grain Quality and Genetic;Improvement of the Corps, Shihezi 832000, China.
  • Received:2021-04-09 Online:2022-06-25 Published:2022-07-04
  • Contact: Chan-juan ZHANG,Xin-an ZHOU E-mail:15738397164@163. com;zhangchanjuan@caas.cn;zhouocri@sina.com;zhangchanjuan@caas.cn


Distinguishing elite germplasm with high nitrogen-use-efficiency is critical to high-yield breeding in soybean. In this study, we employed hydroponic assay and evaluated biomass and tolerance index of low nitrogen stress of 78 accessions at two nitrogen levels, i.e. normal (7.5mmol/L) and low (0.75mmol/L). Based on performance of aboveground dry weight, whole-plant dry weight and low nitrogen tolerance index, 8 elite accessions, including 3 low-nitrogen-tolerant genotypes (2011-X-559, 2011-X-618, 2011-X-639), 3 low-nitrogen-sensitive genotypes (2011-X-472, 2011-X-531, 2011-X-547) and 2 intermediate genotypes (Jiyu 86, Dongnong 53), were distinguished. Furthermore, we focused on the 8 accessions and examined 17 traits, covering chlorophyll content, biomass, nitrogen percentage and root morphology. By means of variation coefficient, Pearson correlation and principal component analysis, 6 traits (composing of dry biomass, total nitrogen percentage, total nitrogen accumulation, total root length, root volume and root surface area) could be considered as the main indicators for screening low nitrogen tolerant soybean germplasm at seedling stage. Consequently, a low nitrogen tolerant accessions 2011-X-618 and a low nitrogen sensitive accessions 2011-X-531 were further validated. We hoped to shed light on deciphering the mechanism of nitrogen use efficiency and provide excellent germplasm for high yield breeding in soybean.

Key words: soybean, nitrogen use efficiency, low nitrogen tolerance index, biomass, root morphology

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