CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2022, Vol. 44 ›› Issue (3): 515-521.doi: 10.19802/j.issn.1007-9084.2021132
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Pei DU(), Qian WANG(
), Hua LIU, Liu-yang FU, Li QIN, Jing XU, Zhong-xin ZHANG, Chen-yu LI, Wen-zhao DONG, Bing-yan HUANG, Xin-you ZHANG(
)
Received:
2021-04-30
Online:
2022-06-25
Published:
2022-07-04
Contact:
Xin-you ZHANG
E-mail:dupei2005@163.com;wang453343550@126.com;haasz@126.com
CLC Number:
Pei DU, Qian WANG, Hua LIU, Liu-yang FU, Li QIN, Jing XU, Zhong-xin ZHANG, Chen-yu LI, Wen-zhao DONG, Bing-yan HUANG, Xin-you ZHANG. Development and characterization of new interspecific hybrid between diploid wild species Arachis duranensis and A. ipaensis[J]. CHINESE JOURNAL OF OIL CROP SCIENCES, 2022, 44(3): 515-521.
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URL: http://www.jouroilcrops.cn/EN/10.19802/j.issn.1007-9084.2021132
Fig. 1
OS FISH and GISH of Arachis duranensis(PI 497262)and A. ipaensis(PI 468322)Note: A-C: Probe staining of PI 497262 (A), PI 468322 (B) and W1824(C) with TAMRA- DP-1 (red), TAMRA- DP-8 (red), FAM-DP-5 (green) and FAM-DP-7 (green); D: GISH of W1824 with total genomic DNA of A. duranensis (green) and A. ipaensis (red); E: Karyotype of PI 497262, PI 468322 and W1824, OS shows oligonucleotide probe staining FISH, GISH shows genomic in situ hybridization
Fig. 2
DAPI staining and GISH of W1824 at diakinesis during meiosisNote: A and C: DAPI staining; B and D: GISH of W1824 with total genomic DNA of A. duranensis (green) and A. ipaensis (red) at diakinesis, yellow arrows show oligonucleotides probe staining, GISH shows genomic in situ hybridization
Table 1
Chromosomal associations in W1824 during meiosis
F1 | 单价体 Monovalent | 二价体 Bivalent | 三价体 Trivalent | A/B二价体 Bivalent of A/B | A/A二价体 Bivalent of A/A | B/B二价体 Bivalent of B/B | A/B/B三价体 Trivalent of A/B/B |
---|---|---|---|---|---|---|---|
W1824-1 | 13 | 2 | 1 | 2 | 0 | 0 | 1 |
W1824-1 | 14 | 3 | 0 | 2 | 0 | 1 | 0 |
W1824-2 | 14 | 3 | 0 | 2 | 0 | 1 | 0 |
W1824-2 | 9 | 4 | 1 | 2 | 1 | 1 | 1 |
W1824-3 | 11 | 3 | 1 | 3 | 0 | 0 | 1 |
W1824-3 | 7 | 5 | 1 | 3 | 1 | 1 | 1 |
W1824-4 | 8 | 6 | 0 | 4 | 1 | 1 | 0 |
W1824-4 | 16 | 2 | 0 | 2 | 0 | 0 | 0 |
平均 Average | 11.5 | 3.5 | 0.5 | 2.5 | 0.375 | 0.625 | 0.5 |
Table 3
Comparison of interspecific hybrid W1824 with its parents in phenotypic traits
性状 Trait | PI 497262(♀) | PI 468322(♂) | W1824(F1) | t-test (F1/♀) | t-test (F1/♂) |
---|---|---|---|---|---|
主茎高 Height of main stem/cm | 33.33±5.51 | 27.83±4.80 | 160.00±23.30 | 9.31** | 9.62** |
侧枝长Length of first primary branch/cm | 86.00±5.57 | 65.33±6.66 | 160.67±14.29 | 8.43** | 10.47** |
叶面积Leaf area/mm2 | 206.52±28.89 | 481.25±43.42 | 699.57±72.14 | 14.19** | 5.80** |
花瓣颜色 Petal color | 黄Yellow | 橙黄Orange | 黄Yellow | / | / |
株型 Plant type | 蔓生Procumbe | 蔓生Procumbe | 蔓生Procumbe | / | / |
1 | Husted L. Cytological studies on the peanut, Arachis.I. Chromosome number and morphology[J]. Cytologia,1933,5(1):109-117. |
2 |
Raina S N, Mukai Y. Genomic in situ hybridization in Arachis (Fabaceae) identifies the diploid wild progenitors of cultivated (A. hypogaea) and related wild (A. Monticola) peanut species[J]. Plant Syst Evol, 1999, 214(1/2/3/4): 251-262. DOI:10.1007/BF00985743 .
doi: 10.1007/BF00985743 |
3 |
Krapovickas A, Gregory W C. Taxonomía del género Arachis (Leguminosae)[J]. Bonplandia, 2007, 16: 1. DOI:10.30972/bon.160158 .
doi: 10.30972/bon.160158 |
4 |
Robledo G, Lavia G I, Seijo G. Species relations among wild Arachis species with the A genome as revealed by FISH mapping of rDNA loci and heterochromatin detection[J]. Theor Appl Genet, 2009, 118(7): 1295-1307. DOI:10.1007/s00122-009-0981-x .
doi: 10.1007/s00122-009-0981-x |
5 |
Robledo G, Seijo G. Species relationships among the wild B genome of Arachis species (section Arachis) based on FISH mapping of rDNA loci and heterochromatin detection: a new proposal for genome arrangement[J]. Theor Appl Genet, 2010, 121(6): 1033-1046. DOI:10.1007/s00122-010-1369-7 .
doi: 10.1007/s00122-010-1369-7 |
6 |
Seijo G, Lavia G I, Fernandez A, et al. Genomic relationships between the cultivated peanut (Arachis hypogaea, Leguminosae) and its close relatives revealed by double GISH[J]. Am J Bot, 2007, 94(12): 1963-1971. DOI:10.3732/ajb.94.12.1963 .
doi: 10.3732/ajb.94.12.1963 |
7 |
Bertioli D J, Cannon S B, Froenicke L, et al. The genome sequences of Arachis duranensis and Arachis ipaensis, the diploid ancestors of cultivated peanut[J]. Nat Genet, 2016, 48(4): 438-446. DOI:10.1038/ng.3517 .
doi: 10.1038/ng.3517 |
8 |
Stalker H T. Utilizing wild species for peanut improvement[J]. Crop Sci, 2017, 57(3): 1102-1120. DOI:10.2135/cropsci2016.09.0824 .
doi: 10.2135/cropsci2016.09.0824 |
9 |
Zhang L, Yang X, Tian L, et al. Identification of peanut (Arachis hypogaea) chromosomes using a fluorescence in situ hybridization system reveals multiple hybridization events during tetraploid peanut formation[J]. New Phytol, 2016, 211(4): 1424-1439. DOI:10.1111/nph.13999 .
doi: 10.1111/nph.13999 |
10 |
Du P, Cui C, Liu H, et al. Development of an oligonucleotide dye solution facilitates high throughput and cost-efficient chromosome identification in peanut[J]. Plant Methods, 2019, 15: 69. DOI:10.1186/s13007-019-0451-7 .
doi: 10.1186/s13007-019-0451-7 |
11 |
Bertioli D J, Jenkins J, Clevenger J, et al. The genome sequence of segmental allotetraploid peanut Arachis hypogaea [J]. Nat Genet, 2019, 51(5): 877-884. DOI:10.1038/s41588-019-0405-z .
doi: 10.1038/s41588-019-0405-z |
12 |
Zhuang W, Chen H, Yang M, et al. The genome of cultivated peanut provides insight into legume karyotypes, polyploid evolution and crop domestication[J]. Nat Genet, 2019, 51(5): 865-876. DOI:10.1038/s41588-019-0402-2 .
doi: 10.1038/s41588-019-0402-2 |
13 |
Feldman M, Levy A A. Genome evolution in allopolyploid wheat: a revolutionary reprogramming followed by gradual changes[J]. J Genet Genomics, 2009, 36(9): 511-518. DOI:10.1016/s1673-8527(08)60142-3 .
doi: 10.1016/s1673-8527(08)60142-3 |
14 |
Singh A K, Smartt J. The genome donors of the groundnut/peanut (Arachis hypogaea L.) revisited[J]. Genet Resour Crop Evol, 1998, 45(2): 113-116. DOI:10.1023/A: 1008640631719 .
doi: 10.1023/A: 1008640631719 |
15 |
Mallikarjuna N, Jadhav D R, Reddy K, et al. Screening new Arachis amphidiploids, and autotetraploids for resistance to late leaf spot by detached leaf technique[J]. Eur J Plant Pathol, 2012, 132(1): 17-21. DOI:10.1007/s10658-011-9859-2 .
doi: 10.1007/s10658-011-9859-2 |
16 |
Chen X, Li H, Pandey M K, et al. Draft genome of the peanut A-genome progenitor (Arachis duranensis) provides insights into geocarpy, oil biosynthesis, and allergens[J]. PNAS, 2016, 113(24): 6785-6790. DOI:10.1073/pnas.1600899113 .
doi: 10.1073/pnas.1600899113 |
17 |
杜培, 张新友, 李丽娜, 等. 珍珠豆型花生品种白沙1016核型分析[J]. 中国油料作物学报, 2013, 35(3): 257-261. DOI:10.7505/j.issn.1007-9084.2013.03.005 .
doi: 10.7505/j.issn.1007-9084.2013.03.005 |
18 | 付留洋, 李丽娜, 李文静, 等. 花生栽培种(Arachis hypogaea L.)与野生种Arachis macedoi杂种F1细胞遗传分析[J]. 中国油料作物学报, 2016, 38(3): 300-306. |
19 |
杜培, 刘华, 李丽娜, 等. 基于顺序GISH-FISH花生栽培种的染色体分析[J]. 中国农业科学, 2015, 48(9): 1854-1863. DOI:10.3864/j.issn.0578-1752.2015.09.19 .
doi: 10.3864/j.issn.0578-1752.2015.09.19 |
20 |
Yant L, Hollister J D, Wright K M, et al. Meiotic adaptation to genome duplication in Arabidopsis arenosa [J]. Curr Biol, 2013, 23(21): 2151-2156. DOI:10.1016/j.cub.2013.08.059 .
doi: 10.1016/j.cub.2013.08.059 |
21 |
Comai L. The advantages and disadvantages of being polyploid[J]. Nat Rev Genet, 2005, 6(11): 836-846. DOI:10.1038/nrg1711 .
doi: 10.1038/nrg1711 |
22 |
Cifuentes M, Grandont L, Moore G, et al. Genetic regulation of meiosis in polyploid species: new insights into an old question[J]. New Phytol, 2010, 186(1): 29-36. DOI:10.1111/j.1469-8137.2009.03084.x .
doi: 10.1111/j.1469-8137.2009.03084.x |
23 |
Diez C M, Roessler K, Gaut B S. Epigenetics and plant genome evolution[J]. Curr Opin Plant Biol, 2014, 18: 1-8. DOI:10.1016/j.pbi.2013.11.017 .
doi: 10.1016/j.pbi.2013.11.017 |
24 |
Ding M, Chen Z J. Epigenetic perspectives on the evolution and domestication of polyploid plant and crops[J]. Curr Opin Plant Biol, 2018, 42: 37-48. DOI:10.1016/j.pbi.2018.02.003 .
doi: 10.1016/j.pbi.2018.02.003 |
25 |
Bian Y, Yang C W, Ou X F, et al. Meiotic chromosome stability of a newly formed allohexaploid wheat is facilitated by selection under abiotic stress as a spandrel[J]. New Phytol, 2018, 220(1): 262-277. DOI:10.1111/nph.15267 .
doi: 10.1111/nph.15267 |
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