Development and characterization of new interspecific hybrid between diploid wild species Arachis duranensis and A. ipaensis

doi:10.19802/j.issn.1007-9084.2021132

Abstract

Abstract:

Diploid wild species Arachis duranensis (AA) and A. ipaensis (BB) are the most possible ancestors of cultivated peanut. Peanuts synthesized by ancestral species are important materials for studying the origin and evolution. In this study, a new interspecific hybrid W1824 of A. duranensis (PI 497262) and A. ipaensis (PI 468322) was created and identified by hybridization, tissue culture, oligonucleotide probe staining FISH (OS FISH) and genomic in situ hybridization (GISH). Analysis of pollen fertility, meiosis behavior and phenotypic traits showed that W1824 had high sterility, its average chromosome configuration was 0.5 III + 3.5 II + 11.5 I, and its height of main stem, length of first primary branch and leaf area showed super-parent advantage. This indicated that the two accessions PI 497262 and PI 468322 had high cross-compatibility and suggested that the tetraploid peanut synthesized using the two accessions might have significantly higher biological yield than their parents and unstable inheritance in chromosomal level.

Key words: Arachis duranensis, a. ipaensis, interspecific hybrid, characterization, origin

CLC Number:

S565.2

Pei DU, Qian WANG, Hua LIU, Liu-yang FU, Li QIN, Jing XU, Zhong-xin ZHANG, Chen-yu LI, Wen-zhao DONG, Bing-yan HUANG, Xin-you ZHANG. Development and characterization of new interspecific hybrid between diploid wild species Arachis duranensis and A. ipaensis[J]. CHINESE JOURNAL OF OIL CROP SCIENCES, 2022, 44(3): 515-521.

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F₁	单价体 Monovalent	二价体 Bivalent	三价体 Trivalent	A/B二价体 Bivalent of A/B	A/A二价体 Bivalent of A/A	B/B二价体 Bivalent of B/B	A/B/B三价体 Trivalent of A/B/B
W1824-1	13	2	1	2	0	0	1
W1824-1	14	3	0	2	0	1	0
W1824-2	14	3	0	2	0	1	0
W1824-2	9	4	1	2	1	1	1
W1824-3	11	3	1	3	0	0	1
W1824-3	7	5	1	3	1	1	1
W1824-4	8	6	0	4	1	1	0
W1824-4	16	2	0	2	0	0	0
平均 Average	11.5	3.5	0.5	2.5	0.375	0.625	0.5

F₁	不育花粉 Sterile pollen	可育花粉 Fertile pollen	花粉活力 Pollen viability/%
W1824-1	531	14	2.57
W1824-2	453	8	1.74
W1824-3	261	9	3.33
W1824-4	366	14	3.68

性状 Trait	PI 497262（♀）	PI 468322（♂）	W1824（F₁）	t-test （F₁/♀）	t-test （F₁/♂）
主茎高 Height of main stem/cm	33.33±5.51	27.83±4.80	160.00±23.30	9.31**	9.62**
侧枝长Length of first primary branch/cm	86.00±5.57	65.33±6.66	160.67±14.29	8.43**	10.47**
叶面积Leaf area/mm²	206.52±28.89	481.25±43.42	699.57±72.14	14.19**	5.80**
花瓣颜色 Petal color	黄Yellow	橙黄Orange	黄Yellow	/	/
株型 Plant type	蔓生Procumbe	蔓生Procumbe	蔓生Procumbe	/	/

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