Cloning, bioinformatics analysis and function identification of two soybean ERD15 genes

doi:10.19802/j.issn.1007-9084.2021140

Abstract

Abstract:

ERD15 （Early Responsive to Dehydration 15）, as a transcription factor, binds to NRP-B promoter and activates NRP-B-mediated cell death. This family consists of six members, of which two members, GmERD15a and GmERD15b, are mainly studied in this study. The functions of GmERD15a and GmERD15b genes were preliminarily explored through cloning, bioinformatics analysis, tissue expression analysis and stress resistance identification in yeast transient system. The results show that the full length of GmERD15a is 378 bp, and the full length of GmERD15b is 318 bp. GmERD15a has the highest homology with GmERD15c, followed by JrERD15. GmERD15b has the highest homology with GmERD15f, followed by VaERD15. Both GmERD15a and GmERD15b are hydrophilic proteins. GmERD15a and GmERD15b were the highest expression levels in 20 d embryos and roots. GmERD15a and GmERD15b were sensitive to drought stress and salt stress. The study was expected to provide a theoretical basis for further study of the function and mechanism of the two genes.

Key words: soybean, ERD15, bioinformatics analysis, preliminary functional identification

CLC Number:

S565.1

Sha-sha CAO, Nan WU, Li-ping WANG, Xiao-yu LIU, Wei-qi WANG, Gui-feng ZHANG, Fa-wei WANG, Xiao-wei LI. Cloning, bioinformatics analysis and function identification of two soybean ERD15 genes[J]. CHINESE JOURNAL OF OIL CROP SCIENCES, 2022, 44(4): 790-797.

Figures/Tables 11

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性质 Property	GmERD15a	GmERD15b
分子量/Da Molecular weight	14 696.47	11 895.83
理论等电点（pI） Theoretical isoelectric point	5.00	3.82
分子式 Molecular formula	C₆₆₇H₉₈₄N₁₇₈O₁₉₂S₄	C₅₃₆H₇₆₉N₁₃₁O₁₇₆S₁
原子总数 Total number of atoms	2025	1613
不稳定系数 Instability coefficient	45.97	52.91
脂肪系数 Fat factor	60.80	67.81
亲疏水性平均系数 Average coefficient of hydrophilicity and hydrophobicity	-0.728	-0.521
负电荷残基总数 Total number of negatively charged residues	21	22
正电荷残基总数 Total number of positively charged residues	16	4

定位Localization	GmERD15a	GmERD15b
细胞质 Cytoplasm	52.2 %	47.8 %
细胞核 Nucleus	34.8 %	21.7 %
线粒体 Mitochondria	8.7 %	26.1 %
分泌系统的囊泡 Vesicles of the secretory system	4.3 %	-
质膜 Plasma membrane	-	4.3 %

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