CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2022, Vol. 44 ›› Issue (4): 790-797.doi: 10.19802/j.issn.1007-9084.2021140

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Cloning, bioinformatics analysis and function identification of two soybean ERD15 genes

Sha-sha CAO(), Nan WU, Li-ping WANG, Xiao-yu LIU, Wei-qi WANG, Gui-feng ZHANG, Fa-wei WANG, Xiao-wei LI()   

  1. Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development, College of Life Sciences, Jilin Agricultural University, Changchun 130118, China
  • Received:2021-05-08 Online:2022-08-25 Published:2022-08-30
  • Contact: Xiao-wei LI E-mail:1178212936@qq.com;xiaoweili1206@163.com


ERD15 (Early Responsive to Dehydration 15), as a transcription factor, binds to NRP-B promoter and activates NRP-B-mediated cell death. This family consists of six members, of which two members, GmERD15a and GmERD15b, are mainly studied in this study. The functions of GmERD15a and GmERD15b genes were preliminarily explored through cloning, bioinformatics analysis, tissue expression analysis and stress resistance identification in yeast transient system. The results show that the full length of GmERD15a is 378 bp, and the full length of GmERD15b is 318 bp. GmERD15a has the highest homology with GmERD15c, followed by JrERD15. GmERD15b has the highest homology with GmERD15f, followed by VaERD15. Both GmERD15a and GmERD15b are hydrophilic proteins. GmERD15a and GmERD15b were the highest expression levels in 20 d embryos and roots. GmERD15a and GmERD15b were sensitive to drought stress and salt stress. The study was expected to provide a theoretical basis for further study of the function and mechanism of the two genes.

Key words: soybean, ERD15, bioinformatics analysis, preliminary functional identification

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