Effect of water stress in different phases on photosynthetic characteristics of drought-avoidant soybean

doi:10.19802/j.issn.1007-9084.2021215

Abstract

Abstract:

Drought is one of the major natural disasters that limit the formation of soybean yield. Application of drought-avoidant varieties is an effective way to alleviate the loss of soybean yield under drought stress. Drought avoidance resistance refers to the ability of plants to maintain high water potential under drought stress. To better understand the mechanism of drought avoidance, two soybean hybrids with contrasting drought avoidance, Liaodou 14 （drought-avoidant） and Liaodou 21 （drought-sensitive） under water stress were evaluated, in order to reveal the photosynthetic regulation mechanism of drought-avoidant genotype soybean. The results showed that water control in each period and soil moisture significantly affected the net photosynthetic rate （Pn）, transpiration rate （Tr）, and stomatal conductance （Gs） of soybean plants. Drought limited the plant’s Pn, Tr, Gs, and intercellular CO₂concentration （Ci）. In general, the average Tr of Liaodou 21 was higher than that of Liaodou 14. With the increase in soil water content, the intercellular CO₂ concentration of plants generally gradually increased. The initial fluorescence （F₀ ） value of soil drought （W1） treatment was higher than other water treatments. The F₀ value was the lowest when the water was controlled at pod stage （R3-R5）. Drought stress reduced the maximum photochemical efficiency （Fv/Fm）, electrons transport rate （ETR） and actual photochemical efficiency （Φ_PSⅡ ） of plants. When soil moisture was controlled at the same stage, the maximum fluorescence yield （Fm） and Fv/Fm of Liaodou 14 were higher than those of Liaodou 21. On the drought stress condition, Liaodou 14 can maintain a lower transpiration rate and higher Fm and Fv/Fm value than that of Liaodou 21. This could be an important photosynthetic factor for drought-avoidant genotype to resist drought stress.

Key words: soybean, water stress, drought- avoidant variety, photosynthesis characteristics

CLC Number:

S565.1

Chun-juan YAN, Shu-hong SONG, Chang-ling WANG, Xu-gang SUN, Yong-qiang CAO, Li-jun ZHANG, Li ZHANG, Xiao-yang HUO, Wen-bin WANG. Effect of water stress in different phases on photosynthetic characteristics of drought-avoidant soybean[J]. CHINESE JOURNAL OF OIL CROP SCIENCES, 2022, 44(5): 1048-1056.

Figures/Tables 10

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品种 Variety	水分 Water	控水时期 Stage of water controlled
品种 Variety	水分 Water	营养生长期 Seedling stage （V4-R1）	花期 Flowering stage （R1-R3）	结荚期 Podding stage （R3-R5）	鼓粒期 Pod filling stage （R5-R6）
辽豆14 Liaodou 14	W1	5.18 ^Cc	5.02^Bc	5.05 ^Cc	11.77 ^Cc
	W2	8.67 ^Bb	11.32^Bb	12.45^Bb	13.80 ^Cc
	W3	12.79 ^Aa	23.39^Aa	22.12^Aa	26.15^Aa
辽豆21 Liaodou 21	W1	4.78 ^Cc	4.87^Bc	4.25 ^Cc	13.99 ^Cc
	W2	9.35 ^Bb	11.63^Bb	16.98^ABb	15.32^BCc
	W3	12.48 ^Aa	27.77^Aa	22.25^Aa	20.19^Bb
平均值Mean
辽豆14 Liaodou 14		8.88 ^Aa	13.25^Aa	13.21^Aa	17.24^Aa
辽豆21 Liaodou 21		8.87 ^Aa	14.75^Aa	14.49^Aa	16.50^Bb
W1		4.98 ^Cc	4.95 ^Cc	4.65^Bc	12.88 ^Bb
W2		9.01 ^Bb	11.47 ^Bb	14.72^Ab	14.56^ABb
W3		12.64^Aa	25.58^Aa	22.19^Aa	23.17^Aa
方差分析（ANOVA）
品种 Varieties		ns	ns	ns	*
水 Water		***	*	*	***
品种×水 V×W		ns	ns	ns	ns

品种 Variety	水分 Water	控水时期 Stage of water controlled
品种 Variety	水分 Water	营养生长期 Seedling stage （V4-R1）	花期 Flowering stage （R1-R3）	结荚期 Podding stage （R3-R5）	鼓粒期 Pod filling stage （R5-R6）
辽豆14 Liaodou 14	W1	2.24 ^Bc	2.39^Bb	2.08 ^Cd	2.02 ^Dc
	W2	3.52 ^Ab	5.03^Bb	6.08 ^ABCbc	2.66 ^CDc
	W3	4.76 ^Aa	13.98 ^Aa	7.38 ^ABab	7.19 ^Aa
辽豆21 Liaodou 21	W1	1.96 ^Bc	2.50^Bb	4.24 ^BCcd	2.75 ^CDc
	W2	3.99 ^Aab	5.76^Bb	5.61 ^ABCbc	4.4B ^Cb
	W3	4.55 ^Aa	13.16 ^Aa	9.02 ^Aa	5.81 ^ABab
平均值 Mean
辽豆14 Liaodou 14		3.51 ^Aa	7.13 ^Aa	5.18^Ab	3.95^Ab
辽豆21 Liaodou 21		3.72 ^Aa	7.14 ^Aa	6.29 ^Aa	4.32^Aa
W1		2.10 ^Cc	2.45^Bc	3.16^Bc	2.38^Bc
W2		3.76 ^Bb	5.39^Bb	5.85 ^Ab	3.53^Bb
W3		4.65^Aa	13.57 ^Aa	8.20 ^Aa	6.50^Aa
方差分析（ANOVA）
品种 Varieties		ns	ns	*	*
水 Water		***	***	***	***
品种×水 V×W		ns	ns	ns	ns

品种 Variety	水分 Water	控水时期 Stage of water controlled
品种 Variety	水分 Water	营养生长期 Seedling stage （V4-R1）	花期 Flowering stage （R1-R3）	结荚期 Podding stage （R3-R5）	鼓粒期 Pod filling stage （R5-R6）
辽豆14 Liaodou 14	W1	59.17^Bb	62.49^Bb	40.03 ^Cc	80.00 ^Cd
	W2	94.36 ^ABb	133.91^Bb	81.52 ^BCc	114.57^Ccd
	W3	200.48^Aa	451.11^Aa	290.09 ^Aa	366.24^Aa
辽豆21 Liaodou 21	W1	49.55^Bb	63.40^Bb	49.00^Cc	115.21^Ccd
	W2	109.24^ABab	159.47^Bb	183.93 ^ABb	193.14^BCc
	W3	123.46^ABab	411.80^Aa	266.26 ^Aab	274.24^ABb
平均值（means）
辽豆14 Liaodou 14		118.01^Aa	215.84^Aa	137.21^Aa	186.94^Aa
辽豆21 Liaodou 21		94.08^Aa	211.55^Aa	166.39^Aa	194.20^Aa
W1		54.36 ^Bb	62.94^Bb	44.51^Bc	97.61^Bc
W2		101.80 ^ABab	146.69^Bb	132.73^Bb	153.86^Bb
W3		161.97 ^Aa	431.46^Aa	278.17^Aa	320.24^Aa
方差分析（ANOVA）
品种 Varieties		ns	ns	ns	ns
水 Water		*	***	***	***
品种×水 V×W		ns	ns	ns	*

品种 Variety	水分 Water	控水时期 Stage of water controlled
品种 Variety	水分 Water	营养生长期 Seedling stage （V4-R1）	花期 Flowering stage （R1-R3）	结荚期 Podding stage （R3-R5）	鼓粒期 Pod filling stage （R5-R6）
辽豆14 Liaodou 14	W1	229.21^Aa	105.8 ^Cc	97.56^Bb	128.00 ^Cb
	W2	216.32^Aa	115.59 ^ABab	112.34^Bb	156.80 ^BCb
	W3	238.45^Aa	106.98 ^Cc	238.76^Aa	244.69 ^Aa
辽豆21 Liaodou 21	W1	209.88^Aa	115.9 ^ABab	119.46^Bb	168.42 ^ABCb
	W2	221.02^Aa	112.66 ^Bb	208.07^Aa	230.34 ^ABa
	W3	198.34^Aa	118.39 ^Aa	222.93^Aa	239.85 ^ABa
平均值 Mean
辽豆14 Liaodou 14		227.99^Aa	109.46 ^Bb	149.55 ^Bb	176.50 ^Ab
辽豆21 Liaodou 21		209.75^Aa	115.65 ^Aa	183.49 ^Aa	212.87 ^Aa
W1		219.54^Aa	110.85^Ab	108.51 ^Cc	148.21 ^Bc
W2		218.67^Aa	114.13^Aa	160.20 ^Bb	193.57 ^ABb
W3		218.40^Aa	112.69 ^Aab	230.85 ^Aa	242.27 ^Aa
方差分析（ANOVA）
品种 Varieties		ns	***	*	*
水 Water		ns	*	***	**
品种×水 V×W		ns	***	*	ns

指标 Index	营养生长期控水 Water controlled at seedling stage （V4-R1）			花期控水 Water controlled at flowering stage （R1-R3）			结荚期控水 Water controlled at podding stage（R3-R5）			鼓粒期控水 Water controlled at pod filling stage （R5-R6）
指标 Index	Pn	Tr	Gs	Pn	Tr	Gs	Pn	Tr	Gs	Pn	Tr	Gs
Tr	0.93^**			0.98^**			0.90^**			0.97^**
Gs	0.90^**	1.00^**		0.97^**	1.00^**		0.96^**	0.85^*		0.98^**	1.00^**
Ci	0.01	0.34	0.40	0.20	0.05	0.02	0.92^**	0.79^*	0.97^**	0.82^*	0.93^**	0.90^**