CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2022, Vol. 44 ›› Issue (5): 1123-1129.doi: 10.19802/j.issn.1007-9084.2021235

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Methylene blue and β-carotene double fading method in soybean breeding of lipoxygenase free

Xian-xu WANG1(), Hui-ming FAN1, Ran OU2, Lei WANG2,3, Sui WANG1, Yan JIANG1,4, Shao-dong WANG1,4()   

  1. 1.Key Laboratory of Soybean Biology of Education Ministry, Northeast Agricultural University, Harbin 150030, China
    2.MOA Key Laboratory of Biology and Genetic Improvement of Soybean,Nanjing Agricultural University,Nanjing 210095,China
    3.Mudanjiang Soybean Research Center, Mudanjiang 157041, China
    4.National Research Center of Soybean Engineering and Technology, Harbin 150028, China
  • Received:2021-09-08 Online:2022-10-25 Published:2022-10-31
  • Contact: Shao-dong WANG E-mail:wangxianxu186@163.com;wsdhlj@neau.edu.cn


The development of screening and identification methods for three isozyme lacking characteristics of lipoxygenase (lox-1,2,3) is significance to the breeding and phenotypic identification of odorless soybean with lipoxygenases lacking in F2 soybean seeds. In this study, methylene blue and β-carotene fading reactions at different pH values of three isoenzymes Lox-1,2,3 were used to accurately identify four phenotypes of Lox-1, 2, 3 wild type, Lox-1, 2, 3 free, Lox-1, 2 free, and Lox-3 free in F2 generation by trace sampling. The identification had no impacts on breeding of the selected individuals with target traits and could provide technical support for breeding and phenotypic identification of new soybean varieties with null lipoxygenases deficiency.

Key words: F2 soybean seeds, breeding of lipoxygenase free, trace sampling, rapid detection, methylene blue and β-carotene fading reactions

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