CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2022, Vol. 44 ›› Issue (6): 1199-1209.doi: 10.19802/j.issn.1007-9084.2021259

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Genetic transformation and resistance evaluation of glyphosate resistance gene in Brassica napus

Shuai DU1(), Li-li WAN2, Zhuan-rong WANG2, Yi XU1, Deng-feng HONG1, Guang-sheng YANG1()   

  1. 1.National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China
    2.Institute of Crop, Wuhan Academy of Agricultural Sciences, Wuhan 430065, China
  • Received:2021-10-14 Online:2022-12-25 Published:2022-11-24
  • Contact: Guang-sheng YANG E-mail:1308976927@qq.com;gsyang@mail.hzau.edu.cn


A novel glyphosate-resistant gene (designated as I. variabilis-EPSPS?) was cloned from bacterium of Isoptericola variabilis. In this research, I.variabilis-EPSPS* was overexpressed in Brassica napus L. cv Yu 127, under the control of constitutive CaMV35S promoter. Seeds from I.variabilis-EPSPS* transgenic plants could germinate and grow well in the presence of increasing concentrations of glyphosate(40-80 mg L-1). In contrast, wild type line had significantly reduced shoot and root length. qRT-PCR indicated that expression level of I.variabilis-EPSPS* increased after isopropylamine salt (Roundup) treatment, and while expression remained unchanged. After spraying 600× dilution of 41% glyphosate Roundup (1/3 recommended dose in field), the cumulative amount of shikimic acid in I.variabilis-EPSPS* transgenic T1 plants was lower than wild type. Additionally, agronomic performance demonstrated no significant difference in silique length, seeds per silique, and 1000-seed weight (etc.) between I.variabilis-EPSPS* E1T1 and wild type in greenhouse. Foliar glyphosate application in field showed that glyphosate at concentration of 759.8 mg L-1 had no detrimental effect on the growth of I.variabilis-EPSPS* plants. Furthermore, the glyphosate-sprayed transgenic plants were preriodically monitored until maturity, and it was observed that they exhibited normal growth and morphological characteristics similar to those of WT which was not spayed with glyphosate in the greenhouse. In summary, the I.variabilis-EPSPS* gene and its transgenic glyphosate-resistant B. napus L. line E1 could be the new materials for genetical modified rapeseed breeding resistant to glyphosate herbicide.

Key words: I.variabilis-EPSPS*genes, Brassica napus, transgene, herbicide resistance

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