CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2022, Vol. 44 ›› Issue (6): 1182-1189.doi: 10.19802/j.issn.1007-9084.2021260

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Genetic improvement and application of resistance to clubroot in male parent of Brassica napus hybrid Shengguang 168

Mei XIONG1(), Guang-sheng YANG1,2, Deng-feng HONG1,2, Zhao-yang WANG1()   

  1. 1.National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China
    2.Hongshan Laboratory, Wuhan 430070, China
  • Received:2021-10-15 Online:2022-12-25 Published:2022-11-24
  • Contact: Zhao-yang WANG E-mail:1454026298@qq.com;wzyyhhn@163.com


To improve rapeseed resistance to clubroot, Huashuang 5R (carrying PbBa8.1 locus) and Huayouza 62R (carrying CRb locus) were used as donor parents for transferring resistant genes into Y522R. The strategy was combined with successive backcross and marker-assisted selection. By the process, 3 near isogenic lines Y522R PbBa8.1, Y522R CRb and Y522R PbBa8.1+CRb (containing PBba8.1, CRb, and PbBa8.1+CRb respectively) were obtained, with more than 90% genetic background recover rate. Subsequently, 3 clubroot resistant new hybrids Shengguang 168CR PbBa8.1, Shengguang 168CR CRb and Shengguang 168CR PbBa8.1+CRb were derived from these near isogenic lines and RG430A (female parent of Shengguang 168). The clubroot resistance of the above lines (as Y522R PbBa8.1, Y522R CRb, Y522R PbBa8.1+CRb,Shengguang 168CR PbBa8.1, Shengguang 168CR CRb and Shengguang 168CR PbBa8.1+CRb ) was identified by using Race 4 (the dominant physiological race in China). Results showed that Y522R CRb, Y522R PbBa8.1+CRb and their hybrids (Shengguang 168CR CRb and Shengguang 168CR PbBa8.1+CRb ) had immunity to Race 4. Although Y522R PbBa8.1 and hybrid Shengguang 168CR PbBa8.1 were susceptible to Race 4, their levels of the resistance were still significantly higher than those of the control (Y522R and Shengguang 168). Evaluation of agronomic traits showed that no significant differences were observed between the improved lines and Y522R, neither between the improved hybrids and Shengguang 168. The traits included flowering days, plant height, effective branches, silique length, seeds per silique and thousand seeds weight. Thus laid an important foundation for cultivation of excellent rape varieties resistant to clubroot.

Key words: Brassica napus L., clubroot disease, PbBa8.1 resistance locus, CRb resistance locus, resistant breeding

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