Abstract:

Lectin receptor like protein kinases belong to the receptor like protein kinases （RLKs） family, which play an important role in plant disease resistance and defense response, growth and development, intracellular signal transduction and abiotic stress response. Transcriptome sequencing was performed on soybean GmNFYB1, and the differentially expressed gene GmLecRlk （Glyma.07G005700） was obtained. Its length of open reading frame was 546 bp, encoding 181 amino acids. Protein domain analysis showed that GmLecRlk contained two serine/threonine kinase domains. It belongs to a G type lectin receptor protein kinase. The results of real-time quantitative PCR showed that GmLecRlk gene was expressed in root, stem, leaf and pod of soybean, and the highest expression level was found in root. Under 200 mmol/L NaCl treatment, the mRNA abundance of GmLecRlk decreased first and then increased, and reached the maximum value at 12 h, indicating that the gene was involved in the response of soybean to salt stress. Agrobacterium tumefaciens K599 was used to obtain GmLecRlk overexpressed transgenic hair-root complex plants. Under salt stress, the survival rate of overexpressed soybean hair-root complex plants was higher than that of control plants. GmLecRlk gene was heterologous expressed in Arabidopsis thaliana, and the germination rate, greening rate and root length of transgenic Arabidopsis thaliana under salt stress were higher than those of wild-type Arabidopsis. In conclusion, GmLecRlk is involved in the response of soybean to salt stress, and the overexpression of GmLecRlk gene can improve the salt tolerance of soybean and Arabidopsis thaliana, providing a new approach and theoretical guidance for breeding and improving salt-resistant soybean varieties.

Key words: soybean, salt stress, receptor-like protein kinases, Arabidopsis