Allelopathy of Brassica napus straw aqueous extract on germination and growth of rice

doi:10.19802/j.issn.1007-9084.2021267

Abstract

Abstract:

In order to explore the effect of Brassica napus straw on rice in the oil-rice cropping system, Fengyou 737, Xiangzayou 553 and Zhongshuang11, three rape cultivars were selected to prepare the straw aqueous extract with different concentrations （ 0, 25%, 50% and 75%）, the germination and growth of two rice seeds（Wuyou 308 and C Liangyou 343）were investigated, and the results suggested that rape straw aqueous extract increased the rice seedling height and fresh weight generally. Meanwhile, rape straw aqueous extract affected the germination ratio, seedling root length of rice in a concentration-dependent way. The germination ratio, germination index of rice seeds and seedling root length were increased at low-concentration of 25%, but decreased at the higher-concentrations of 50% and 75%. As the concentration increased, the aqueous extract of Xiangzayou 553 increased the soluble protein content and significantly reduced the activity of superoxide dismutase （T-SOD） and peroxidase （POD） of Wuyou308 seedlings. In C Liangyou 343 seedlings, the T-SOD and POD activity significantly increased while the protein content greatly decreased; at 25% concentration, the malondialdehyde （MDA） content also noticeably decreased. The aqueous extract of Zhongshuang 11 at 75% concentration increased the MDA content and decreased the POD activity of Wuyou 308 seedlings. Fengyou 737 at 25% concentration greatly decreased the soluble protein content of C Liangyou343 seedlings but increased the activity of T-SOD and POD. In conclusion, the higher the concentration of the rape straw aqueous extract, the stronger its allelopathic effects on rice germination and seedling growth. However, allelopathic effects of extracts from different rape varieties on different rice varieties were different. Our study suggested that the combination of Fengyou 737 and Wuyou 308 is more suited for an oil-rice system.

Key words: Brassica napus, straw, aqueous extract, seed germination, seedling growth

CLC Number:

S565.4

Zi-xiang ZOU, Ying LIU, Ding-gang ZHOU, Li-li LIU, Da-wei ZHANG, Jin-feng WU, Mei LI, Ming-li YAN. Allelopathy of Brassica napus straw aqueous extract on germination and growth of rice[J]. CHINESE JOURNAL OF OIL CROP SCIENCES, 2022, 44(6): 1286-1295.

Figures/Tables 7

Table 1

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处理 Treatments	水浸液浓度 Aqueous extract concentration /%	五优308 Wuyou 308		C两优343 C Liangyou 343
处理 Treatments	水浸液浓度 Aqueous extract concentration /%	发芽率 Germination rate /%	发芽指数 Germination index	发芽率 Germination rate /%	发芽指数 Germination index
CK	0	38.0±8.7^abc	2.9±0.9^abc	17.3±13.4^ab	1.0±0.8^a
沣油737 Fengyou737	25	37.3±7.6^abc	3.1±0.5^ab	20.0±7.8^a	1.1±0.5^a
	50	33.3±6.2^bc	2.7±0.6^bc	11.3±3.0^ab	0.8±0.1^a
	75	36.7±5.3^abc	2.9±0.4^abc	16.7±3.3^ab	1.1±0.3^a
湘杂油553 Xiangzayou553	25	43.3±10.5^ab	3.1±0.6^ab	12.7±2.8^ab	0.7±0.2^a
	50	36.0±2.8^bc	2.3±0.2^bc	10.0±7.8^ab	0.5±0.5^a
	75	35.3±6.1^bc	2.3±0.4^bc	9.3±8.3^ab	0.5±0.4^a
中双11 Zhongshuang 11	25	46.7±11.1^a	3.6±0.9^a	18.0±7.3^ab	1.1±0.5^a
	50	30.7±10.9^c	2.1±1.0^c	8.7±5.1^b	0.5±0.4^a
	75	34.0±9.5b^c	2.3±0.8^bc	12.7±4.3^ab	0.7±0.3^a

主效应 Main effects	指标 Index	五优308 Wuyou 308		C两优343 C Liangyou 343
主效应 Main effects	指标 Index	F	P	F	P
秸秆品种	发芽率Germination rate	0.241	0.787	1.798	0.180
Straw type	发芽指数Germination index	0.525	0.595	3.241	0.048^*
水浸液浓度	发芽率Germination rate	3.329	0.027^*	2.098	0.113
Aqueous extract concentration	发芽指数Germination index	4.906	0.005^**	2.290	0.090
秸秆品种×水浸液浓度	发芽率Germination rate	0.665	0.678	0.452	0.840
Straw type ×Aqueous extract concentration	发芽指数Germination index	0.804	0.572	0.766	0.600

处理 Treatments	水浸液浓度 Aqueous extract concentration /%	五优308 Wuyou308			C两优343 C Liangyou343
处理 Treatments	水浸液浓度 Aqueous extract concentration /%	苗高 Seedling height /mm	鲜重 Fresh weight /mg	根长 Root length /mm	苗高 Seedling height /mm	鲜重 Fresh weight /mg	根长 Root length /mm
CK	0	70.52±9.51^e	65.36±13.18^fg	87.04±16.14^ab	63.73±21.32^c	65.86±36.36^b	61.67±24.59^abc
沣油737 Fengyou737	25	83.76±7.42^ab	89.80±13.08^bc	89.76±12.47^a	61.72±20.85^c	64.34±30.49^b	63.15±26.01^ab
	50	76.40±6.63^cd	73.30±12.57^ef	91.88±14.65^a	66.92±10.46^abc	73.38±19.37^ab	57.63±11.66^bcd
	75	82.08±6.87^b	87.71±11.70^cd	73.58±16.30^c	66.88±11.69^bc	70.61±22.64^b	53.98±13.85^bcd
湘杂油553 Xiangzayo553	25	83.76±10.18^ab	97.66±16.72^ab	79.60±20.86^bc	77.46±12.19^a	88.66±19.77^a	72.38±19.79^a
	50	81.60±8.13^bc	80.34±14.59^de	75.40±21.89^c	68.50±17.27^abc	71.48±26.18^ab	48.33±16.29^cde
	75	73.08±10.40^de	61.99±12.43^g	31.28±17.04^e	60.55±14.21^c	62.26±26.46^b	36.45±14.37^e
中双11 Zhongshuang11	25	87.76±7.41^a	99.42±15.69^a	91.48±23.97^a	74.43±9.73^ab	89.34±15.10^a	73.04±17.45^a
	50	80.50±13.19^bc	82.79±23.46^cd	77.88±23.83^bc	66.55±14.31^abc	69.99±26.48^ab	44.73±14.31^de
	75	70.00±14.47^e	67.97±14.94^fg	43.21±15.17^d	59.19±12.74^c	59.58±18.80^b	38.13±12.69^e

处理Treatments	水浸液浓度 Aqueous extract concentration /%	化感效应指数Response index （RI）
		五优308 Wuyou 308			C两优343 C Liangyou 343
		苗高 Seedling height	鲜重 Fresh weight	根长 Root length	苗高 Seedling height	鲜重 Fresh weight	根长 Root length
CK	0	—	—	—	—	—	—
沣油737 Fengyou737	25	0.16	0.27	0.03	-0.03	-0.02	0.02
	50	0.08	0.11	0.05	0.05	0.10	-0.07
	75	0.14	0.25	-0.15	0.05	0.07	-0.12
湘杂油553 Xiangzayou553	25	0.16	0.33	-0.09	0.18	0.26	0.15
	50	0.14	0.19	-0.13	0.07	0.08	-0.22
	75	0.04	-0.05	-0.64	-0.05	-0.05	-0.41
中双11 Zhongshuang 11	25	0.20	0.34	0.05	0.14	0.26	0.16
	50	0.12	0.21	-0.11	0.04	0.06	-0.27
	75	-0.01	0.04	-0.50	-0.07	-0.10	-0.38

处理 Treatments	水浸液浓度 Aqueous extract concentration /%	化感综合效应Synthesis effect		平均Average		排名 Ranking
处理 Treatments	水浸液浓度 Aqueous extract concentration /%	五优308 Wuyou308	C两优343 C Liangyou343	五优308 Wuyou308	C两优343 C Liangyou343	排名 Ranking
CK	0	—	—	—	—	—
沣油737 Fengyou737	25	0.061	0.178	0.016	0.008	1
	50	-0.013	-0.221
	75	0.001	0.066
湘杂油553 Xiangzayou553	25	0.207	-0.122	-0.040	-0.282	3
	50	-0.081	-0.337
	75	-0.246	-0.388
中双11 Zhongshuang 11	25	0.295	0.097	-0.025	-0.141	2
	50	-0.151	-0.321
	75	-0.220	-0.198