CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2022, Vol. 44 ›› Issue (6): 1228-1238.doi: 10.19802/j.issn.1007-9084.2021268

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Construction of high density genetic map and QTL mapping of yield related traits in soybean

Bo WANG(), Ying-ying DONG, Xue FU, He-yu LIU, Xiang-chao ZHANG, Ji LIU, Fei-fei SHI, Xue ZHAO, Ying-peng HAN, Wen-bin LI, Wei-li TENG()   

  1. Institute of Soybean Research, Key Laboratory of Soybean Biology in the Ministry of Education, Key Laboratory of Soybean Biology and Breeding (Genetics) of the Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, Harbin 150030, China
  • Received:2021-10-25 Online:2022-12-25 Published:2022-11-24
  • Contact: Wei-li TENG E-mail:wangbo_181030@163.com;twlneau@163.com


To accelerate molecular marker-assisted breeding for soybean, a recombinant inbred lines (RILs) population (F2:10) containing 168 lines was constructed from Dongnong 42 and Dongnong 50 for constructing a high density genetic map. The two parents are significantly different on yield. QTL analysis was carried out by using the compound interval mapping (CIM) method of R/qtl software combined with phenotypic data on yield-related traits at 6 environments in 2 years. Results showed that 660 316 SNP markers obtained by sequencing could be used to construct a high-density genetic map of soybean containing 6227 bin markers distributed in 20 linkage groups. The general and average map distances were 2739.15 cM and 0.44 cM respectively. 22 QTLs related to yield traits were mapped on 12 chromosomes. QTLs for number of four-seed-pod (NFSP), number of pods per plant (NPPP), seed weight per plant (SWPP), 100-seed weight (HSW) were 5, 4, 5 and 8 respectively. There was a genomic region on each of the chromosomes 3 and 19. They were repeatedly located in 2 years, involving 6 major QTLs, namely qNFSP-19-1 (22.976%), qNFSP-19-2 (11.977%), qNFSP-19-3 (17.203%), qHSW-3-1 (11.346%), qHSW-3-2 (11.346%) and qHSW-3-3 (11.175%), all these QTLs had negative additive effects. 7 QTLs for yield-related traits were newly mapped on chromosomes 3, 7, 11, 12 and 20, among which qHSW-3-3 (14.276%) had the highest phenotypic contribution, including qHSW-3-2 and qHSW-3-3 with repetitive mapping intervals. Compared with the results of previous co-mapping, more QTLs greatly shortened the mapping interval, which showed the accuracy of the high-density genetic map constructed in this study.

Key words: soybean, high density genetic map, number of four-seed-pod, pods per plant, seed weight per plant, 100-seed weight, QTL

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