CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2022, Vol. 44 ›› Issue (6): 1239-1248.doi: 10.19802/j.issn.1007-9084.2021275

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Development and verification of molecular markers for QTLs related to the number of three-seeded pods in soybean

Li-long HOU1(), Hong-wei JIANG2, Xin XIONG1, Hai-yang ZHENG1, Fu-bin CAO1, Ru-ru WEI1, Yi-chao ZHANG1, Yu-xuan ZHAO1, Meng-yao GUO1, Qing-shan CHEN1()   

  1. 1.Northeast Agricultural University, Harbin 150030, China
    2.Jilin Academy of Agricultural Sciences, Changchun, 130033, China
  • Received:2021-10-31 Online:2022-12-25 Published:2022-11-24
  • Contact: Qing-shan CHEN E-mail:hllsbds@126.com;qshchen@126.com


To realize the purpose of marker-assisted selection, InDel markers were developed based on QTL for three-seeded pod number of soybeans, the natural population and breeding population were used to screen the polymorphism of InDel markers and verify the application effect, respectively. Finally, the expression of candidate genes in QTL for three seed-pod number was used to verify the validity of QTL. The results showed that primers INDEL3-311497, INDEL17-440117 and INDEL17-462053 could be employed to select the progeny in the hybrid population of Canadian Danbaidou and Jiyu 4512, INDEL13-24003 could be used in the hybrid population of Qinong 7 and Xindali, INDEL3-250092, INDEL3-311497 and INDEL13-254396 could be used in the hybrid population between Dongfudou 8 and Tongxiangdou 4, INDEL3-240669 and INDEL13-246066 could be used for progeny selection in the hybrid population of Jiyu 441 and Hefeng 55. Two candidate genes, Glyma.03G029800 and Glyma.17G062600, promoted the formation and development of three-seeded pods, while two candidate genes Glyma.17G062000 and Glyma.13G063700 inhibit the formation and development of three-seeded pods. These results indicated that the four QTL loci obtained in our laboratory may be related to the formation of three soybean pods, which also confirmed the effectiveness of InDel markers on breeding population selection.

Key words: soybean, number of three-seeded pods, molecular marker, marker-assisted selection

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