Table 1

Parental names of 4 breeding populations

Table 2

QTL locus information

Table 3

Sampling method of Real-time quantitative RCR

Table 4

Fluorescence quantitative PCR primers

Fig. 1

Three-seeded pods of extreme materials in resource populationNote: **: P≤0.01; L represents the materials with extremely low percentage of three pods in the resource population; H represents the material with extremely high percentage of three pods in the resource population

Table 5

Number of InDel primer bands

Table 6

Analysis table of band type variance of primers in resource population

Fig. 2

Percentage of three-seeded pods in 4 populationsNote: A: Population 1；B: Population 2；C: Population 3； D: Population 4

Fig. 3

Effectiveness of 11 pairs of primers in breeding populationsNote: ● indicates valid, and ○ indicates invalid

Fig. 4

InDel markers correspond to phenotypes of genotypes in breeding populationsNote: * represents significant level P≤0.05, and ** represents significant level P≤0.01； 1 is the paternal genotype, 2 is the maternal genotype, and 3 is the heterozygous genotype

Table 7

Analysis of variance of primer bands in breeding populations

Fig. 5

Characterization of three-seeded pods of fluorescence quantitative materialNote: **: P≤0.01； L represents the materials with extremely low percentage of three pods in the resource population, and H represents the material with extremely high percentage of three pods in the resource population

Fig. 6

Expression analyses of candidate genes in extreme materialsNote: A: Expression of candidate gene Glyma.03G029800; B: Expression of candidate gene Glyma.13G063700; C: Expression of candidate gene Glyma.17G062000; D: Expression of candidate gene Glyma.17G062600