Impact analysis of nitrogen fertilizer reduction on bacterial community structure and function in rhizosphere soil of continuous cropping sesame

doi:10.19802/j.issn.1007-9084.2021282

Abstract

Abstract:

The variety Jinhuangma was used as the test material, and different nitrogen reduction (RSN4, at 0% less on the basis of 105 kg·hm^-2; RSN3, RSN2, RSN1, RSN0 represent 25%, 50%, 75% and 100% reduction of nitrogen fertilizer, respectively) were set to study the effect on the yield of continuous cropping sesame and the bacterial community structure in rhizosphere soil under healthy or bacterial wilt conditions by using high-throughput sequencing technology. The results showed that the yield of sesame with 25% reduction of nitrogen fertilizer (RSN3) did not decrease significantly compared with RSN4 treatment. The α-diversity indexes of Obs, Shannon, Chao1 in RSN2 (e.g. 50% N reduction), were significantly higher by 11.53%, 3.39%, and 12.71% than those in RSN4, respectively, and Shannon index from healthy plants was significantly higher by 1.61% than that from diseased plants, and the interaction of nitrogen reduction and growth state treatment (e.g. healthy and diseased) had a significant effect on Shannon index. Compared with RSN3, the relative abundance of Actinobacteria with RSN1 (75% N reduction) significantly increased by 39.70%, but Firmicutes decreased by 30.60%, respectively. Rrelative abundance of Acidobacteria, Cyanobacteria and Nitrospirae of rhizosphere soil from healthy plants were significantly higher by 32.42%, 155.26% and 38.54% than those from diseased plants respectively, while Firmicutes decreased significantly by 47.47% compared with diseased plants. Relative abundance of Bacillus and Massilia in RSN3 increased by 119.10% and 87.18% compared with RSN2, respectively, and that of Paenibacillus in RSN3 increased by 59.29%-193.42% compared with RSN0, RSN1 and RSN2. Relative abundance of Candidatus_Solibacter and unidentified_Nostocales from rhizosphere soil bacteria of healthy plants were 43.68% and 235.90% higher than those of diseased plants (P < 0.05), while the four genera of bacteria from rhizosphere soil of diseased plants (Bacillus, Massilia, Ramlibcter, and Paenibacillus) increased by 64.63%-106.67% than those of healthy plants (P < 0.05). Relative abundance of Carbohydrate_metabolism from rhizosphere soil of healthy plants increased by 0.94% compared with the diseased plants (P < 0.05), while the relative abundance of membrane_transport from rhizosphere soil of healthy plants was 2.37% lower than that of diseased plants (P < 0.05). Compared with RSN0 treatment, the relative abundance of metabolism_of_cofactors_ and_vitamins and nucleoside_metabolism of RSN3 increased by 1.78% and 3.63% (P < 0.05), while the relative abundance of translation, lipid_metabolism, and folding_sorting_and_degradation decreased significantly by 4.58%, 4.52% and 7.74%, respectively (P < 0.05). The contributions of three main environmental factors (pH, available potassium and available zinc) of the bacterial community change were 3.91%, 5.93% and 6.94%, respectively. In conclusion, appropriately reducing the amount of nitrogen fertilizer by 25% affects little on sesame yield, and improves the structure and function of rhizosphere soil bacterial community, which is conducive to the healthy growth of continuous cropping sesame in red soil.

Key words: continuous cropping sesame, nitrogen reduction, bacterial community structure, high throughput sequencing

CLC Number:

S565.3

Rui-qing WANG, Zhi-hua ZHANG, Feng-juan LYU, Hong-xin LIN, Lin-gen WEI, Yun-ping XIAO. Impact analysis of nitrogen fertilizer reduction on bacterial community structure and function in rhizosphere soil of continuous cropping sesame[J]. CHINESE JOURNAL OF OIL CROP SCIENCES, 2022, 44(6): 1307-1319.

Figures/Tables 11

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处理 Treatment	自由度 Df	总方差 Sums Of Sqs	均方差 Mean Sqs	F检验值 F.Model	决定系数 R²	Pr（>F）
RSD-RSH	1（28）	0.177（1.835）	0.177（0.066）	2.697	0.088（0.912）	0.002
RSN2-RSN4	1（10）	0.098（0.724）	0.098（0.072）	1.354	0.119（0.881）	0.157
RSN2-RSN0	1（10）	0.048（0.678）	0.048（0.068）	0.707	0.066（0.934）	0.832
RSN2-RSN1	1（10）	0.063（0.676）	0.063（0.068）	0.934	0.085（0.915）	0.527
RSN2-RSN3	1（10）	0.133（0.543）	0.133（0.054）	2.442	0.196（0.804）	0.017
RSN4-RSN0	1（10）	0.088（0.776）	0.088（0.078）	1.139	0.102（0.898）	0.316
RSN4-RSN1	1（10）	0.057（0.774）	0.057（0.077）	0.734	0.068（0.932）	0.702
RSN4-RSN3	1（10）	0.062（0.641）	0.062（0.064）	0.961	0.088（0.913）	0.496
RSN0-RSN1	1（10）	0.072（0.728）	0.072（0.073）	0.995	0.091（0.910）	0.428
RSN0-RSN3	1（10）	0.115（0.595）	0.115（0.059）	1.936	0.162（0.838）	0.011
RSN1-RSN3	1（10）	0.086（0.593）	0.086（0.059）	1.446	0.126（0.874）	0.136

功能 Function	生长状态 Growth state		减氮处理 Nitrogen reduction					RS	RSN	RS×RSN
功能 Function	RSD	RSH	RSN0	RSN1	RSN2	RSN3	RSN4	RS	RSN	RS×RSN
碳水化合物代谢Carbohydrate_metabolism	10.65b	10.75a	10.66	10.74	10.66	10.68	10.77	*	ns	ns
膜运输Membrane_transport	9.72a	9.49b	9.66	9.59	9.55	9.69	9.55	**	ns	ns

氨基酸代谢Amino_acid_metabolism	9.87	9.79	9.89ab	9.89ab	9.90a	9.74ab	9.74b	ns	ns	ns
脂质代谢Lipid_metabolism	4.17	4.11	4.20ab	4.27a	4.16abc	4.01c	4.08bc	ns	ns	ns
折叠、分类和降级Folding，_sorting_and_degradation	2.88	2.86	2.97a	2.89ab	2.92ab	2.74b	2.84ab	ns	ns	ns
异源生物降解和代谢 Xenobiotics_biodegradation_and_metabolism	2.33	2.25	2.34ab	2.38a	2.31ab	2.21b	2.23ab	ns	ns	ns
其他氨基酸的代谢Metabolism_of_other_amino_acids	1.87	1.86	1.87ab	1.89a	1.86b	1.86b	1.86b	ns	ns	ns
新陈代谢Metabolism	1.81	1.79	1.82ab	1.83a	1.8ab	1.78b	1.78b	ns	ns	ns

翻译Translation	8.55	8.56	8.73a	8.54ab	8.67ab	8.34b	8.50ab	ns	ns	ns
辅助因子和维生素的代谢 Metabolism_of_cofactors_and_vitamins	3.40	3.44	3.38b	3.41ab	3.43ab	3.44a	3.44a	ns	ns	ns
核苷酸代谢Nucleotide_metabolism	3.07	3.09	3.03b	3.04ab	3.07ab	3.14a	3.11ab	ns	ns	ns
细胞运动Cell_motility	2.08	2.09	2.08ab	2.04b	2.07b	2.15a	2.10ab	ns	ns	ns

转录Transcription	1.76	1.76	1.75	1.75	1.75	1.76	1.77	ns	ns	ns
细胞群落原核生物Cellular_community_prokaryotes	2.21	2.16	2.23	2.18	2.19	2.16	2.16	ns	ns	ns

复制和修复Replication_and_repair	7.14	7.19	7.07	7.10	7.15	7.28	7.21	ns	ns	ns
能量代谢Energy_metabolism	4.62	4.67	4.59	4.62	4.66	4.68	4.67	ns	ns	ns
信号转导Signal_transduction	3.71	3.76	3.70	3.70	3.71	3.81	3.77	ns	ns	ns
酶家族Enzyme_families	2.74	2.83	2.74	2.75	2.77	2.85	2.83	ns	ns	ns
聚糖生物合成和代谢 Glycan_biosynthesis_and_metabolism	2.67	2.73	2.62	2.64	2.65	2.82	2.79	ns	ns	ns
运输和分解代谢Transport_and_catabolism	2.28	2.30	2.27	2.27	2.29	2.31	2.31	ns	ns	ns