CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2022, Vol. 44 ›› Issue (6): 1259-1266.doi: 10.19802/j.issn.1007-9084.2021294

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Soybean GmDof2.2 improved the sensitivity of transgenic tobacco to salt stress

Ying ZHAI1(), Ting-ting MA1, Jia-qi HE1, Jun ZHANG2, Ming-yang LI1, Jiong-xin CHEN1, Hai-wei YU1, Shan-shan LI1, Tian-guo SUN1   

  1. 1.Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, College of Life Science and Agro-Forestry,Qiqihar University, Qiqihar 161006
    2.Branch of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences, Qiqihar 161005
  • Received:2021-11-18 Online:2022-12-25 Published:2022-11-24


Dof transcription factors are involved in plant response to abiotic stresses. In this research, GmDof2.2 was cloned for salt tolerance identification. Real-time quantitative PCR results showed that GmDof2.2 could respond to abiotic stresse in soybean seedlings. Theopen reading frame of GmDof2.2 was 867 bp, which encodes a protein of 288 amino acids. The molecular weight of GmDof2.2protein was 31.4 kDa, and isoelectric point was 10.23. The GmDof2.2protein sequence contains a conserved Dof domain. The promoter region of GmDof2.2 contains three types of stress-related cis-elements (ARE, MBS and WUN-motif) and three types of hormone-related cis-elements (ABRE, P-box and TCA-element). GmDof2.2 was constructed into a plant expression vector and transformed into tobacco. Four GmDof2.2 transgenic tobacco lines (OE1-OE4) were obtained. The expression level of GmDof2.2 in OE1 was the highest, followed by OE2. After salt stress treatment, the wilting degree of transgenic tobacco was higher than that of wild tobacco, the contents of chlorophyll, soluble sugar and proline in transgenic tobacco leaves were significantly lower than that of wild tobacco, while the content of malondialdehyde was higher than that of wild tobacco. These results showed that GmDof2.2 heterologous expression increased the sensitivity to salt stress in transgenic tobacco. This study provided theoretical basis for the research and application of soybean Dof transcription factor resistance mechanism.

Key words: soybean, transcription factor, transgenic tobacco, salt tolerance

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