Soybean GmDof2.2 improved the sensitivity of transgenic tobacco to salt stress

doi:10.19802/j.issn.1007-9084.2021294

Abstract

Abstract:

Dof transcription factors are involved in plant response to abiotic stresses. In this research, GmDof2.2 was cloned for salt tolerance identification. Real-time quantitative PCR results showed that GmDof2.2 could respond to abiotic stresse in soybean seedlings. Theopen reading frame of GmDof2.2 was 867 bp, which encodes a protein of 288 amino acids. The molecular weight of GmDof2.2protein was 31.4 kDa, and isoelectric point was 10.23. The GmDof2.2protein sequence contains a conserved Dof domain. The promoter region of GmDof2.2 contains three types of stress-related cis-elements （ARE, MBS and WUN-motif） and three types of hormone-related cis-elements （ABRE, P-box and TCA-element）. GmDof2.2 was constructed into a plant expression vector and transformed into tobacco. Four GmDof2.2 transgenic tobacco lines （OE1-OE4） were obtained. The expression level of GmDof2.2 in OE1 was the highest, followed by OE2. After salt stress treatment, the wilting degree of transgenic tobacco was higher than that of wild tobacco, the contents of chlorophyll, soluble sugar and proline in transgenic tobacco leaves were significantly lower than that of wild tobacco, while the content of malondialdehyde was higher than that of wild tobacco. These results showed that GmDof2.2 heterologous expression increased the sensitivity to salt stress in transgenic tobacco. This study provided theoretical basis for the research and application of soybean Dof transcription factor resistance mechanism.

Key words: soybean, transcription factor, transgenic tobacco, salt tolerance

CLC Number:

S565.1

Ying ZHAI, Ting-ting MA, Jia-qi HE, Jun ZHANG, Ming-yang LI, Jiong-xin CHEN, Hai-wei YU, Shan-shan LI, Tian-guo SUN. Soybean GmDof2.2 improved the sensitivity of transgenic tobacco to salt stress[J]. CHINESE JOURNAL OF OIL CROP SCIENCES, 2022, 44(6): 1259-1266.

Figures/Tables 7

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顺式元件 Cis-element	预测功能 Predicted function	序列 Sequence	数目 Amount
ARE	厌氧诱导元件 Anaerobic induction element	AAACCA	4
MBS	干旱诱导的MYB结合位点 MYB binding site involved in drought-inducibility	CAACTG	1
WUN-motif	创伤响应元件 Wound responsive element	AAATTACT	2
ABRE	脱落酸响应元件 ABA responsive element	ACGTG	2
P-box	赤霉素响应元件 Gibberellin-responsive element	CCATCTTTTT	1
TCA-element	水杨酸响应元件 Salicylic acid responsive element	CCATCTTTTT	1

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