CHINESE JOURNAL OF OIL CROP SCIENCES ›› 2023, Vol. 45 ›› Issue (1): 46-55.doi: 10.19802/j.issn.1007-9084.2021306

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Chloroplast DNA polymorphism of Brassica napus using SSR markers

Bi-yun CHEN(), Pei-jun LYU, Kun XU, Xiao-ming WU()   

  1. Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China
  • Received:2021-12-07 Online:2023-02-25 Published:2023-03-03
  • Contact: Xiao-ming WU E-mail:chenbiyun@oilcrops.cn;wuxm@ oilcrops.cn


Chloroplast genome is characterized by its maternal inheritance, haplotype, high conservation, and distinct intraspecific differences. To facilitate germplasm collection and introduction of rapeseed (Brassica napus L.), chloroplast DNA diversity was investigated using different B. napus varieties. Global collection of 287 B. napus materials was evaluated using specific chloroplast SSR markers. Among the 15 chloroplast specific SSR-specific primer pairs, 5 pairs produced 19 polymorphic bands with the average of 3.8. Using the 19 polymorphic bands, 287 materials were divided into 14 haplotypes. Among them, the more prevalent haplotypes of H01, H02 and H03 occupied 74.91%, 13.59%, and 4.88%, respectively, while the other 11 haplotypes (H04-H14) accounted for 6.62%. Haplotype H02, a unique haplotype to China is cytoplasmic type of Polima or Shaan2A. In the foreign B. napus varieties, those from Russia or Sweden had more abundant haplotypes than those from other countries. Therefore introduction of resources from Russia or Sweden could efficiently broaden the genetic diversity background of Chinese B. napus varieties. Moreover, a rapid identification of Polima and Shaan2A cytoplasmic types was also established through 2 specific bands amplified by MF-4 and ccmp2 primer pairs, which provided methods for identification of Polima and Shaan2A cytoplasmic type.

Key words: Brassica napus L., chloroplast SSR markers, DNA polymorphism, haplotype

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